LOCUS       BBa_K180016             1371 bp    DNA     linear       08-May-2024
DEFINITION  Rock-paper-scissors - Paper secondary plasmid [lux pR, LacI 
            generator]
ACCESSION   BBa_K180016
VERSION     BBa_K180016.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        20..25
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    53..53
                     /label=start
     misc_feature    1..55
                     /label=BBa_R0062
     promoter        1..55
                     /label=BBa_R0062
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    1171..1209
                     /label=LVA
     CDS             82..1209
                     /label=lacI-LVA
     misc_feature    82..1209
                     /label=BBa_C0012
     misc_feature    1210..1234
                     /label=Help:Barcodes
     CDS             82..1209
                     /label=BBa_C0012
     stem_loop       1254..1297
                     /label=stem_loop
     misc_feature    1243..1322
                     /label=BBa_B0010
     terminator      1243..1322
                     /label=BBa_B0010
     stem_loop       1338..1357
                     /label=T7 TE
     misc_feature    1364..1364
                     /label=stop
     polyA_site      1358..1371
                     /label=polya
     misc_feature    1331..1371
                     /label=BBa_B0012
     terminator      1331..1371
                     /label=BBa_B0012
     terminator      1243..1371
                     /label=BBa_B0015
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta
       61 gagaaagagg agaaatacta gatggtgaat gtgaaaccag taacgttata cgatgtcgca
      121 gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt
      181 tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac
      241 cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt
      301 ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg
      361 ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg
      421 gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac
      481 caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc
      541 tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc
      601 gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt
      661 tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt
      721 cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg
      781 caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg
      841 ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta
      901 gtgggatacg acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa
      961 caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc
     1021 caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg
     1081 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     1141 cgacaggttt cccgactgga aagcgggcag gctgcaaacg acgaaaacta cgctttagta
     1201 gcttaataac tctgatagtg ctagtgtaga tctctactag agccaggcat caaataaaac
     1261 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     1321 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
//