LOCUS       BBa_K1808011            1743 bp    DNA     linear       29-May-2024
DEFINITION  ALDH1 regulated by the TAC promoter
ACCESSION   BBa_K1808011
VERSION     BBa_K1808011.1  
FEATURES             Location/Qualifiers
     promoter        1..40
                     /label=Ptac
     promoter        7..12
                     /label=-35
     promoter        29..33
                     /label=-10
     misc_feature    36..61
                     /label=lacO1 site
     promoter        1..61
                     /label=BBa_K864400
     RBS             70..82
                     /label=RBS-3\Weak
     RBS             76..79
                     /label=RBS
     misc_feature    70..82
                     /label=BBa_B0032
     RBS             70..82
                     /label=BBa_B0032
     CDS             89..1606
                     /label=ALDH1 6 His tagged
     misc_feature    1585..1603
                     /label=6 His
     CDS             89..1606
                     /label=BBa_K1808004
     misc_feature    70..1606
                     /label=BBa_S05303
     stem_loop       1626..1669
                     /label=stem_loop
     misc_feature    1615..1694
                     /label=BBa_B0010
     terminator      1615..1694
                     /label=BBa_B0010
     stem_loop       1710..1729
                     /label=T7 TE
     misc_feature    1736..1736
                     /label=stop
     polyA_site      1730..1743
                     /label=polya
     misc_feature    1703..1743
                     /label=BBa_B0012
     terminator      1703..1743
                     /label=BBa_B0012
     terminator      1615..1743
                     /label=BBa_B0015
     misc_feature    70..1743
                     /label=BBa_S05306
ORIGIN
        1 gagctgttga caattaatca tcggctcgta taatgtgtgg aattgtgagc ggataacaat
       61 ttactagagt cacacaggaa agtactagat gtcctcaggg gcgaatggct cgagtaagtc
      121 tgcatcacat aaaatcaaat tcactaaact gtttattaac ggtgaatttg tagattcgat
      181 cagtggtaac accttcgata ccatcaaccc tgcgactgaa gaagtgctgg caacagttgc
      241 ggagggacgc aaagaagata ttgacctggc tgtgaaagct gcgcgcgagg cctttgataa
      301 cggtccctgg ccgcggatgt ctggggaggc gcggcgcaag atcatgctga aatttgccga
      361 tttaattgac gaaaacgccg acgaactgac gacacttgaa gtaattgatg gcgggaagtt
      421 gtttggtcct gtgcgtcact ttgaggttcc ggtgtctagc gatacttttc gttacttcgc
      481 cggtgcagca gataaaattc gtggggcgac gctgaaaatg tcttcgaata tccaggcgta
      541 cactttacgc gagccgattg gcgtagtagg tcacatcatc ccgtggaacg gtccagcctt
      601 catgtttgca accaaggtag ctccggcact cgcggcggga tgcaccatgg tgattaagcc
      661 agcggaacac acaccactga cggtgctgtt tctggcgcac ctgagcaaac tcgcgggagt
      721 tccggacggt gttatcaacg ttgtcaacgg gtttggcaaa acggctggcg cggcggttag
      781 ctcacatatg gacatcgaca tggtgacctt caccggatca accgaggtgg gtcgtacggt
      841 gatgcaggct gccgctctga gcaacctgaa gccggtatcg ctggagctgg ggggcaaaag
      901 cccgctgatc gtttttgacg atgccgatgt ggacaaagct gccgaatttg ccatcttggg
      961 taattttacc aacaaaggag aaatgtgtgt tgctggaagc cgtgtttttg tgcaggaggg
     1021 catccatgat gtcttcgtga aaaagctgga aggggctgtt aaagcctggg cgacacgtga
     1081 cccattcgac ctggccaccc gtcatggccc gcaaaacaat aaacagcaat atgacaaagt
     1141 actcagttgt atcaatcatg gaaaaaaaga aggggcgacc ctggttacgg gcggcaagcc
     1201 ctttgggaaa aaaggctatt atatcgaacc taccctgttc acgaatgtca ccgatgacat
     1261 gaccatcgcg aaagaagaaa tctttgggcc tgtcattagc gtgttaaaat tcaaaacagt
     1321 ggaggaggtt attaaacgtg ccaacgcgac gaagtatggt ctcgcgtcag gcgtgttcac
     1381 taagaatatt gatgtagtga atacggtgag ccgttcaatc cgtgcgggtg cggtttgggt
     1441 caattgttat ttagccttgg accgggacgc acctcatggt gggtataaaa tgtcggggtt
     1501 cggccgcgaa caggggttag aagcgctgga acattattta cagattaaaa ccgtggcaac
     1561 gccaatctac gatagcccat ggctccatca ccatcaccat cactaatact agagccaggc
     1621 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     1681 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
     1741 ata
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