BBa_K864400 1 BBa_K864400 Ptac, trp & lac regulated promoter 2012-09-24T11:00:00Z 2015-05-08T01:13:37Z Created from oligos ordered from SigmaAldrich. Oligo sequences: Ptac+ AATTCGCGGCCGCTTCTAGAGGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTA Ptac- CTAGTAAATTGTTATCCGCTCACAATTCCACACATTATACGAGCCGATGATTAATTGTCAACAGCTCCTCTAGAAGCGGCCGCG Released HQ 2013 The Ptac promoter is a functional hybrid promoter, derived from the trp and lac promoters, that are regulated by trp and lac [1]. This part also exist together with lacI, part [[Part:BBa_K180000|BBa_K180000]] [1] Proc. Natl. Acad. Sci. USA, Vol. 80, pp. 21-25 false false _1124_ 0 9827 9 In stock false Oligos ordered from SigmaAldrich were annealed to form a double stranded DNA segment, ready to be ligated into any BioBrick backbone. false Erik Lundin annotation2197237 1 -35 range2197237 1 7 12 annotation2197236 1 Ptac range2197236 1 1 40 annotation2197240 1 lacO1 site range2197240 1 36 61 annotation2197238 1 -10 range2197238 1 29 33 BBa_K1808011 1 BBa_K1808011 ALDH1 regulated by the TAC promoter 2015-09-15T11:00:00Z 2015-09-16T12:13:35Z Artemisia annua This is a device that is an ALDH1 generator in a pSB1C3 plasmid backbone. It expressed ALDH1 if induced by IPTG and not repressed by tryptophan. This construct is an element of TCD iGEM 2015 for the synthesis of the anti-malarial drug precursor dihydroartemisinic acid in E. coli. The ALDH1 gene encodes the enzyme aldehyde dehydrogenase 1, which catalyses the conversion of (11R)-dihydroartemisinic aldehyde to dihydroartemisinic acid as well as the conversion of artemisinic aldehyde to artemisinic acid with NAD+ as the substrate. Artemisinic acid and dihydroartemisinic acid can be extracted from E. coli cells through lysis and chemically converted to the anti-malarial artemisinin. This construct was generated via 3A assembly and is optimised for expression in E. coli cells. false false _2233_ 26124 26124 9 false This construct was generated via 3A assembly and is optimised for expression in E. coli cells. false Remsha Afzal component2461589 1 BBa_S05306 component2461574 1 BBa_K864400 annotation2461589 1 BBa_S05306 range2461589 1 70 1743 annotation2461574 1 BBa_K864400 range2461574 1 1 61 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_K1808004 1 BBa_K1808004 ALDH1 from Artemisia annua, 6 His tagged 2015-09-15T11:00:00Z 2015-09-18T06:12:06Z Artemisia annua This is the coding region of the ALDH1 gene from Artemisia annua with added biobrick suffix, prefix and a 6 His tag in a pSB1C3 plasmid backbone. This part is an element of TCD iGEM 2015 set of parts for the synthesis of the anti-malarial drug precursor dihydroartemisinic acid in E. coli. The ALDH1 gene encodes the enzyme aldehyde dehydrogenase 1, which catalyses the conversion of (11R)-dihydroartemisinic aldehyde to dihydroartemisinic acid as well as the conversion of artemisinic aldehyde to artemisinic acid with NADP+ as the substrate. Artemisinic acid and dihydroartemisinic acid can be extracted from E. coli cells through lysis and chemically converted to the anti-malarial artemisinin. This part is codon optimised for BioBrick 3A assembly and expression in E. coli cells as well as protein purification. false false _2233_ 26113 26113 9 true This part is BioBrick 3A assembly, E. coli codon and synthesis optimised. This part is 6 His tagged to allow protein purification. false Arnas Petrauskas annotation2461246 1 6 His range2461246 1 1497 1515 annotation2461243 1 ALDH1 6 His tagged range2461243 1 1 1518 BBa_B0032 1 BBa_B0032 RBS.3 (medium) -- derivative of BBa_0030 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _41_44_48_46_1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;RBS-2&quot; in figure 4-14 of thesis). <P> Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation1710 1 RBS range1710 1 7 10 annotation1709 1 RBS-3\Weak range1709 1 1 13 annotation7027 1 BBa_B0032 range7027 1 1 13 BBa_S05303 1 BBa_S05303 B0032:K1808004 2015-09-15T11:00:00Z 2015-09-16T11:34:09Z false false _9_ 26125 26125 9 false false D??ire Gannon component2461386 1 BBa_K1808004 component2461382 1 BBa_B0032 annotation2461386 1 BBa_K1808004 range2461386 1 20 1537 annotation2461382 1 BBa_B0032 range2461382 1 1 13 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_S05306 1 BBa_S05306 S05303:B0015 2015-09-15T11:00:00Z 2015-09-16T11:47:49Z false false _9_ 26113 26113 9 false false Arnas Petrauskas component2461458 1 BBa_S05303 component2461465 1 BBa_B0015 annotation2461465 1 BBa_B0015 range2461465 1 1546 1674 annotation2461458 1 BBa_S05303 range2461458 1 1 1537 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_B0032_sequence 1 tcacacaggaaag BBa_S05306_sequence 1 tcacacaggaaagtactagatgtcctcaggggcgaatggctcgagtaagtctgcatcacataaaatcaaattcactaaactgtttattaacggtgaatttgtagattcgatcagtggtaacaccttcgataccatcaaccctgcgactgaagaagtgctggcaacagttgcggagggacgcaaagaagatattgacctggctgtgaaagctgcgcgcgaggcctttgataacggtccctggccgcggatgtctggggaggcgcggcgcaagatcatgctgaaatttgccgatttaattgacgaaaacgccgacgaactgacgacacttgaagtaattgatggcgggaagttgtttggtcctgtgcgtcactttgaggttccggtgtctagcgatacttttcgttacttcgccggtgcagcagataaaattcgtggggcgacgctgaaaatgtcttcgaatatccaggcgtacactttacgcgagccgattggcgtagtaggtcacatcatcccgtggaacggtccagccttcatgtttgcaaccaaggtagctccggcactcgcggcgggatgcaccatggtgattaagccagcggaacacacaccactgacggtgctgtttctggcgcacctgagcaaactcgcgggagttccggacggtgttatcaacgttgtcaacgggtttggcaaaacggctggcgcggcggttagctcacatatggacatcgacatggtgaccttcaccggatcaaccgaggtgggtcgtacggtgatgcaggctgccgctctgagcaacctgaagccggtatcgctggagctggggggcaaaagcccgctgatcgtttttgacgatgccgatgtggacaaagctgccgaatttgccatcttgggtaattttaccaacaaaggagaaatgtgtgttgctggaagccgtgtttttgtgcaggagggcatccatgatgtcttcgtgaaaaagctggaaggggctgttaaagcctgggcgacacgtgacccattcgacctggccacccgtcatggcccgcaaaacaataaacagcaatatgacaaagtactcagttgtatcaatcatggaaaaaaagaaggggcgaccctggttacgggcggcaagccctttgggaaaaaaggctattatatcgaacctaccctgttcacgaatgtcaccgatgacatgaccatcgcgaaagaagaaatctttgggcctgtcattagcgtgttaaaattcaaaacagtggaggaggttattaaacgtgccaacgcgacgaagtatggtctcgcgtcaggcgtgttcactaagaatattgatgtagtgaatacggtgagccgttcaatccgtgcgggtgcggtttgggtcaattgttatttagccttggaccgggacgcacctcatggtgggtataaaatgtcggggttcggccgcgaacaggggttagaagcgctggaacattatttacagattaaaaccgtggcaacgccaatctacgatagcccatggctccatcaccatcaccatcactaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1808011_sequence 1 gagctgttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaatttactagagtcacacaggaaagtactagatgtcctcaggggcgaatggctcgagtaagtctgcatcacataaaatcaaattcactaaactgtttattaacggtgaatttgtagattcgatcagtggtaacaccttcgataccatcaaccctgcgactgaagaagtgctggcaacagttgcggagggacgcaaagaagatattgacctggctgtgaaagctgcgcgcgaggcctttgataacggtccctggccgcggatgtctggggaggcgcggcgcaagatcatgctgaaatttgccgatttaattgacgaaaacgccgacgaactgacgacacttgaagtaattgatggcgggaagttgtttggtcctgtgcgtcactttgaggttccggtgtctagcgatacttttcgttacttcgccggtgcagcagataaaattcgtggggcgacgctgaaaatgtcttcgaatatccaggcgtacactttacgcgagccgattggcgtagtaggtcacatcatcccgtggaacggtccagccttcatgtttgcaaccaaggtagctccggcactcgcggcgggatgcaccatggtgattaagccagcggaacacacaccactgacggtgctgtttctggcgcacctgagcaaactcgcgggagttccggacggtgttatcaacgttgtcaacgggtttggcaaaacggctggcgcggcggttagctcacatatggacatcgacatggtgaccttcaccggatcaaccgaggtgggtcgtacggtgatgcaggctgccgctctgagcaacctgaagccggtatcgctggagctggggggcaaaagcccgctgatcgtttttgacgatgccgatgtggacaaagctgccgaatttgccatcttgggtaattttaccaacaaaggagaaatgtgtgttgctggaagccgtgtttttgtgcaggagggcatccatgatgtcttcgtgaaaaagctggaaggggctgttaaagcctgggcgacacgtgacccattcgacctggccacccgtcatggcccgcaaaacaataaacagcaatatgacaaagtactcagttgtatcaatcatggaaaaaaagaaggggcgaccctggttacgggcggcaagccctttgggaaaaaaggctattatatcgaacctaccctgttcacgaatgtcaccgatgacatgaccatcgcgaaagaagaaatctttgggcctgtcattagcgtgttaaaattcaaaacagtggaggaggttattaaacgtgccaacgcgacgaagtatggtctcgcgtcaggcgtgttcactaagaatattgatgtagtgaatacggtgagccgttcaatccgtgcgggtgcggtttgggtcaattgttatttagccttggaccgggacgcacctcatggtgggtataaaatgtcggggttcggccgcgaacaggggttagaagcgctggaacattatttacagattaaaaccgtggcaacgccaatctacgatagcccatggctccatcaccatcaccatcactaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_S05303_sequence 1 tcacacaggaaagtactagatgtcctcaggggcgaatggctcgagtaagtctgcatcacataaaatcaaattcactaaactgtttattaacggtgaatttgtagattcgatcagtggtaacaccttcgataccatcaaccctgcgactgaagaagtgctggcaacagttgcggagggacgcaaagaagatattgacctggctgtgaaagctgcgcgcgaggcctttgataacggtccctggccgcggatgtctggggaggcgcggcgcaagatcatgctgaaatttgccgatttaattgacgaaaacgccgacgaactgacgacacttgaagtaattgatggcgggaagttgtttggtcctgtgcgtcactttgaggttccggtgtctagcgatacttttcgttacttcgccggtgcagcagataaaattcgtggggcgacgctgaaaatgtcttcgaatatccaggcgtacactttacgcgagccgattggcgtagtaggtcacatcatcccgtggaacggtccagccttcatgtttgcaaccaaggtagctccggcactcgcggcgggatgcaccatggtgattaagccagcggaacacacaccactgacggtgctgtttctggcgcacctgagcaaactcgcgggagttccggacggtgttatcaacgttgtcaacgggtttggcaaaacggctggcgcggcggttagctcacatatggacatcgacatggtgaccttcaccggatcaaccgaggtgggtcgtacggtgatgcaggctgccgctctgagcaacctgaagccggtatcgctggagctggggggcaaaagcccgctgatcgtttttgacgatgccgatgtggacaaagctgccgaatttgccatcttgggtaattttaccaacaaaggagaaatgtgtgttgctggaagccgtgtttttgtgcaggagggcatccatgatgtcttcgtgaaaaagctggaaggggctgttaaagcctgggcgacacgtgacccattcgacctggccacccgtcatggcccgcaaaacaataaacagcaatatgacaaagtactcagttgtatcaatcatggaaaaaaagaaggggcgaccctggttacgggcggcaagccctttgggaaaaaaggctattatatcgaacctaccctgttcacgaatgtcaccgatgacatgaccatcgcgaaagaagaaatctttgggcctgtcattagcgtgttaaaattcaaaacagtggaggaggttattaaacgtgccaacgcgacgaagtatggtctcgcgtcaggcgtgttcactaagaatattgatgtagtgaatacggtgagccgttcaatccgtgcgggtgcggtttgggtcaattgttatttagccttggaccgggacgcacctcatggtgggtataaaatgtcggggttcggccgcgaacaggggttagaagcgctggaacattatttacagattaaaaccgtggcaacgccaatctacgatagcccatggctccatcaccatcaccatcactaa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1808004_sequence 1 atgtcctcaggggcgaatggctcgagtaagtctgcatcacataaaatcaaattcactaaactgtttattaacggtgaatttgtagattcgatcagtggtaacaccttcgataccatcaaccctgcgactgaagaagtgctggcaacagttgcggagggacgcaaagaagatattgacctggctgtgaaagctgcgcgcgaggcctttgataacggtccctggccgcggatgtctggggaggcgcggcgcaagatcatgctgaaatttgccgatttaattgacgaaaacgccgacgaactgacgacacttgaagtaattgatggcgggaagttgtttggtcctgtgcgtcactttgaggttccggtgtctagcgatacttttcgttacttcgccggtgcagcagataaaattcgtggggcgacgctgaaaatgtcttcgaatatccaggcgtacactttacgcgagccgattggcgtagtaggtcacatcatcccgtggaacggtccagccttcatgtttgcaaccaaggtagctccggcactcgcggcgggatgcaccatggtgattaagccagcggaacacacaccactgacggtgctgtttctggcgcacctgagcaaactcgcgggagttccggacggtgttatcaacgttgtcaacgggtttggcaaaacggctggcgcggcggttagctcacatatggacatcgacatggtgaccttcaccggatcaaccgaggtgggtcgtacggtgatgcaggctgccgctctgagcaacctgaagccggtatcgctggagctggggggcaaaagcccgctgatcgtttttgacgatgccgatgtggacaaagctgccgaatttgccatcttgggtaattttaccaacaaaggagaaatgtgtgttgctggaagccgtgtttttgtgcaggagggcatccatgatgtcttcgtgaaaaagctggaaggggctgttaaagcctgggcgacacgtgacccattcgacctggccacccgtcatggcccgcaaaacaataaacagcaatatgacaaagtactcagttgtatcaatcatggaaaaaaagaaggggcgaccctggttacgggcggcaagccctttgggaaaaaaggctattatatcgaacctaccctgttcacgaatgtcaccgatgacatgaccatcgcgaaagaagaaatctttgggcctgtcattagcgtgttaaaattcaaaacagtggaggaggttattaaacgtgccaacgcgacgaagtatggtctcgcgtcaggcgtgttcactaagaatattgatgtagtgaatacggtgagccgttcaatccgtgcgggtgcggtttgggtcaattgttatttagccttggaccgggacgcacctcatggtgggtataaaatgtcggggttcggccgcgaacaggggttagaagcgctggaacattatttacagattaaaaccgtggcaacgccaatctacgatagcccatggctccatcaccatcaccatcactaa BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K864400_sequence 1 gagctgttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaatt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z