BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_J23119
1
BBa_J23119
constitutive promoter family member
2006-08-23T11:00:00Z
2015-08-31T04:08:40Z
Overlap extension of synthetic oligonucleotides
Released HQ 2013
Later
false
true
_52_
0
483
95
In stock
false
N/A
true
John Anderson
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_K1809007
1
BBa_K1809007
Const. Promoter-RBS-MaAFP-DT
2015-08-02T11:00:00Z
2015-08-03T04:05:53Z
Composite of parts BBa_J23119 and BBa_K1809024.
This part contains the constitutive promoter BBa_J23119, an RBS, Macrozarces americanus antifreeze protein (MaAFP), and a double terminator. It produces a high level of expression of the antifreeze protein.
false
false
_2234_
26152
26152
9
false
Used a strong promoter to produce high levels of expression of MaAFP.
false
Edward Dring
component2434104
1
BBa_B0012
component2434099
1
BBa_B0034
component2434097
1
BBa_J23119
component2434102
1
BBa_B0010
component2434101
1
BBa_K1809006
annotation2434099
1
BBa_B0034
range2434099
1
44
55
annotation2434101
1
BBa_K1809006
range2434101
1
62
325
annotation2434104
1
BBa_B0012
range2434104
1
422
462
annotation2434097
1
BBa_J23119
range2434097
1
1
35
annotation2434102
1
BBa_B0010
range2434102
1
334
413
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K1809006
1
BBa_K1809006
MaAFP
2015-08-02T11:00:00Z
2016-01-27T10:33:14Z
Taken from the ocean pout Macrozarces americanus. Pubmed ID: 3840475
Antifreeze proteins (AFPs) are molecules with the unique property of binding and shaping ice crystals, preventing their growth into relatively large cell-lysing structures (Davies and Sykes 1997). They exhibit a property known as thermal hysteresis, whereby their activity decreases the freezing point of water without changing the melting point. This property allows organisms which produce AFPs to survive colder conditions. Additionally, AFPs have shown biolfilm inhibbiting properties, potetnially giving them uses in medical applications.
false
false
_2234_
4206
26152
9
false
Codon optimized the sequence for E. coli and to minimize repetitive sequences; removed illegal restriction sites.
false
Edward Dring
annotation2433990
1
MaAFP
range2433990
1
1
264
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K1809007_sequence
1
ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatgaaaagcgtgattcttacgggcctgctgtttgtgttactgtgtgttgatcatatgaccgccagccagtccgtcgtcgctacgcagctgatcccaatcaatactgccctgacgcccgcgatgatggaaggaaaggtgaccaatccaattgggattccgttcgctgaaatgtcacagatcgtgggcaaacaagtgaacaccccggtggcgaaaggtcagacgctgatgcccaacatggtaaaaacttatgttgccgggaaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_J23119_sequence
1
ttgacagctagctcagtcctaggtataatgctagc
BBa_K1809006_sequence
1
atgaaaagcgtgattcttacgggcctgctgtttgtgttactgtgtgttgatcatatgaccgccagccagtccgtcgtcgctacgcagctgatcccaatcaatactgccctgacgcccgcgatgatggaaggaaaggtgaccaatccaattgggattccgttcgctgaaatgtcacagatcgtgggcaaacaagtgaacaccccggtggcgaaaggtcagacgctgatgcccaacatggtaaaaacttatgttgccgggaaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z