LOCUS       BBa_K1809014            1001 bp    DNA     linear       01-Jun-2024
DEFINITION  Const. Promoter-RBS-BclA-TiAFP-DT
ACCESSION   BBa_K1809014
VERSION     BBa_K1809014.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    64..126
                     /label=BBa_K1423000
     CDS             133..864
                     /label=TiAFP
     CDS             133..864
                     /label=BBa_K1809012
     stem_loop       884..927
                     /label=stem_loop
     misc_feature    873..952
                     /label=BBa_B0010
     terminator      873..952
                     /label=BBa_B0010
     stem_loop       968..987
                     /label=T7 TE
     misc_feature    994..994
                     /label=stop
     polyA_site      988..1001
                     /label=polya
     misc_feature    961..1001
                     /label=BBa_B0012
     terminator      961..1001
                     /label=BBa_B0012
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gaggcgttcg atcctaatct ggtaggaccc acccttccgc cgatcccgcc attcacactg
      121 ccaacatact agatgttttc cgccagttcg ctgctggctg tgattgcgct cgcaatctct
      181 agtgtatctg ctgcggggcc tagtgctgtt cctctcggaa ccgccggcaa ctatgttatc
      241 ttagcgagca ctggggtgtc cactgttcct cagagtgtta ttactggtgc agtgggtgtg
      301 agtccaggta cggcagcgtc actgacgggc ttctccttga tcctctcggg caccggtacc
      361 tttagtacct ccagccaggt gaccggtcaa cttaccggcg cggactacgg cacaccgacc
      421 ccgtcaatcc tcaccaccgc cattggggat atgggcaccg cgtacattaa tgccgcaacc
      481 cgttcggggc cggatttcct ggaaatttat acgggagccc tcggtgggac cactctgctt
      541 cctggtctgt acaaatggac tagctccgtt ggcgcaagcg ccgatttcac gatctctggg
      601 acgtcaaccg atacttggat ttttcaaatt gatggtacat taggtctcgc ggcgggtaag
      661 aaaattacct tggtaggtgg tgctcaggcg aaaaatgtca tttgggtcgt ggccggggcg
      721 gtcaatattg aagtgggcgc caaatttgaa ggcactatcc tggccaaaac cgcggtgacc
      781 ttcaaaacag gatccagcct gaacggtcgt atcctggcgc agacagccgt ggcactgcaa
      841 tcagcgacca ttgtggagaa ataatactag agccaggcat caaataaaac gaaaggctca
      901 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
      961 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
//