LOCUS       BBa_K1809020            1268 bp    DNA     linear       01-Jun-2024
DEFINITION  Const. Promoter-RBS-BclA-DcAFP-DT
ACCESSION   BBa_K1809020
VERSION     BBa_K1809020.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    64..126
                     /label=BBa_K1423000
     CDS             133..1131
                     /label=DcAFP
     CDS             133..1131
                     /label=BBa_K1809018
     stem_loop       1151..1194
                     /label=stem_loop
     misc_feature    1140..1219
                     /label=BBa_B0010
     terminator      1140..1219
                     /label=BBa_B0010
     stem_loop       1235..1254
                     /label=T7 TE
     misc_feature    1261..1261
                     /label=stop
     polyA_site      1255..1268
                     /label=polya
     misc_feature    1228..1268
                     /label=BBa_B0012
     terminator      1228..1268
                     /label=BBa_B0012
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gaggcgttcg atcctaatct ggtaggaccc acccttccgc cgatcccgcc attcacactg
      121 ccaacatact agatgaacat tgagtcctca ttttgcccga ttctgtgtat ttgtatgatc
      181 tttctgtgct taccgaatct gtccgcctcc cagcggtgta acaataatga caagcaggct
      241 ctgctgcaaa tcaaaactgc actgaaaaat ccgacaatta cggattcttg ggtgtcagac
      301 gatgattgct gcggctggga cctggtggaa tgtgatgaaa ctagcaatcg cattatttcg
      361 ttaattatcc aggacgatga agctctgact ggccaaatcc cacctcaagt gggtgatctg
      421 ccgtacttac aagcactgtg gttccgcaag ttgcccaatc tgttcggcaa aattccggaa
      481 gagatcagtg ccctgaagga tctcaagagc ctgcggctga gtagcacgag tttgagcggc
      541 ccagtgcctc tgttttttcc gcaattgacc aaactgactt gcctcgacct gagcttcaat
      601 aaattgctcg gggttatccc tccgcagctg agtacccttc caaatctgaa agcgttgcat
      661 ttagaacgga atgagctgac cggggagatc cccgatatct tcgggaactt cgcaggcagt
      721 ccggacatct atctgtcgca caatcagctg actggattcg tcccaaaaac atttgcgcgt
      781 gccgatccga ttcgtctgga cttttccggg aaccggctgg aaggtgatat tagttttctg
      841 tttggtccaa aaaaacgcct ggaaatgttg gattttagtg gtaatgtgtt aagcttcaat
      901 ttttcacgtg ttcaggaatt tccgccgagt ctgacatatc tggatttaaa ccataaccag
      961 atctcaggaa gcttgagtag cgagttggcc aaactggacc tgcaaacgtt caacgtatcc
     1021 gacaataacc tgtgtggcaa aattccgaca ggtggaaatt tgcagcgctt tgaccgtact
     1081 gcatacttac ataatagctg tctgtgcggc gcgccgctgc cggagtgtta atactagagc
     1141 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     1201 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     1261 cgtttata
//