LOCUS       BBa_K1813021            2327 bp    DNA     linear       10-Jun-2024
DEFINITION  <i>nicD</i> Expression Cassette with LacI Reversed
ACCESSION   BBa_K1813021
VERSION     BBa_K1813021.1  
FEATURES             Location/Qualifiers
     promoter        1..1319
                     /label=BBa_K1813019
     promoter        1328..1367
                     /label=Ptac
     promoter        1334..1339
                     /label=-35
     promoter        1356..1360
                     /label=-10
     misc_feature    1363..1388
                     /label=lacO1 site
     promoter        1328..1388
                     /label=BBa_K864400
     misc_feature    1401..1404
                     /label=conserved
     RBS             1397..1408
                     /label=BBa_B0034
     CDS             1415..2224
                     /label=BBa_K1813007
     stem_loop       2240..2259
                     /label=T7 TE
     misc_feature    2266..2266
                     /label=stop
     polyA_site      2260..2273
                     /label=polya
     misc_feature    2233..2273
                     /label=BBa_B0012
     terminator      2233..2273
                     /label=BBa_B0012
     stem_loop       2294..2316
                     /label=stem_loop
     misc_feature    2282..2327
                     /label=BBa_B0011
     terminator      2282..2327
                     /label=BBa_B0011
     terminator      2233..2327
                     /label=BBa_B0014
     terminator      2233..2327
                     /label=BBa_K823017
     misc_feature    1328..2327
                     /label=BBa_K1813015
ORIGIN
        1 aaataataaa aaagccggat taataatctg gctttttata ttctctctct agtatataaa
       61 cgcagaaagg cccacccgaa ggtgagccag tgtgactcta gtagagatct acactagcac
      121 tatcagagtt attaagctac taaagcgtag ttttcgtcgt ttgcagcctg cccgctttcc
      181 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
      241 gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga
      301 ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc
      361 agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg
      421 gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg
      481 gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg
      541 ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc
      601 cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc
      661 agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat
      721 gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg
      781 atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc
      841 acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc
      901 gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac
      961 accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac
     1021 ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc
     1081 agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt
     1141 tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa
     1201 gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccatctag
     1261 tatttctcct ctttctctag taggcgctat catgccatac cgcgaaaggt tttgcaccat
     1321 actagaggag ctgttgacaa ttaatcatcg gctcgtataa tgtgtggaat tgtgagcgga
     1381 taacaattta ctagagaaag aggagaaata ctagatgagt acctttgtgg caggcggcaa
     1441 cgtatctgcc aacggcattc gtcaacacta tttgcgttat ggcggcaaag gtcacgcttt
     1501 aatcttagtt ccaggcatta catctcctgc catcacatgg ggcttcgtgg ctgaacgctt
     1561 aggtcattac tttgacacct atgtattgga cgttcgtggt cgtggcttat ctagttcagg
     1621 ccctgacttg gattacggca ctgacgcatg cgccgccgac atcccagctt ttgccgcagc
     1681 cttgggtttg gatagttacc acttattagg tcattctatg ggcgcccgtt ttgcaatccg
     1741 cgccgccgca caaggcgccc caggcttgca gcgcttagtg ttagtagacc ctccagtaag
     1801 tggtccaggc cgtcgtgcat acccatcaaa attaccttgg tacgtggatt ctattcgcca
     1861 agctaccgta ggcatgagtg gtgacgatat gcgcgccttc tgtgctactt ggtcagatga
     1921 acagttagct ttacgcgcag agtggttaca cacatgttat gaaccagcca tcgtgcgtgc
     1981 atttgacgat tttcacgaag ttgatattca ccagtattta cctgctgtgc gccagcctgc
     2041 cttattaatg gttgccggtc gtggcggcgt tattgaacca cgtgatattg ccgaaatgcg
     2101 tgagttgaaa ccagacatcc aagtggccta cgttgataat gccggccaca tgatcccatg
     2161 ggatgattta gacggtttct tcgccgcatt cggcgatttc ttagatcacc cattggttta
     2221 ataatactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga
     2281 gagagaatat aaaaagccag attattaatc cggctttttt attattt
//