LOCUS       BBa_K1813030            1246 bp    DNA     linear       29-May-2024
DEFINITION  <i>nicX<i> Expression Cassette
ACCESSION   BBa_K1813030
VERSION     BBa_K1813030.1  
FEATURES             Location/Qualifiers
     promoter        1..40
                     /label=Ptac
     promoter        7..12
                     /label=-35
     promoter        29..33
                     /label=-10
     misc_feature    36..61
                     /label=lacO1 site
     promoter        1..61
                     /label=BBa_K864400
     misc_feature    74..77
                     /label=conserved
     RBS             70..81
                     /label=BBa_B0034
     CDS             88..1143
                     /label=BBa_K1813029
     stem_loop       1159..1178
                     /label=T7 TE
     misc_feature    1185..1185
                     /label=stop
     polyA_site      1179..1192
                     /label=polya
     misc_feature    1152..1192
                     /label=BBa_B0012
     terminator      1152..1192
                     /label=BBa_B0012
     stem_loop       1213..1235
                     /label=stem_loop
     misc_feature    1201..1246
                     /label=BBa_B0011
     terminator      1201..1246
                     /label=BBa_B0011
     terminator      1152..1246
                     /label=BBa_B0014
     terminator      1152..1246
                     /label=BBa_K823017
ORIGIN
        1 gagctgttga caattaatca tcggctcgta taatgtgtgg aattgtgagc ggataacaat
       61 ttactagaga aagaggagaa atactagatg ccagtaagta atgcacagtt aacccagatg
      121 ttcgaacacg tgttaaaatt aagtcgcgtg gatgaaactc agtctgttgc agtgttaaaa
      181 agtcattata gtgaccctcg cactgtgaac gcagctatgg aggcagcaca acgtttgaaa
      241 gctaaggttt atgccgttga attacctgca tttaaccatc caaccgctat gggtaatgat
      301 atgaccgcct attgcggcga taccgcatta actggtaact tagccgctca gcgtgctttg
      361 gaggcagcag acttggttgt ggacacaatg atgttattgc acagtccaga acaggagcag
      421 atcttgaaaa caggcactcg catcttgttg gccgttgaac cacctgaggt gttggcacgc
      481 atgttaccaa ccgaagatga caagcgtcgt gtattggctg ccgaaacctt gttgaaacaa
      541 gcccgcagtt tacatgtgcg ttctaaagcc ggcagtgatt ttcatgcacc attgggtcag
      601 tatccagccg taaccgaata tggttatgct gacgagcctg gccgttggga tcattggcca
      661 tctggctttt tatttacttg gccaaacgag gactcagcag aaggtacctt agtgttggat
      721 gttggcgata ttatcttgcc attcaaaaac tattgccgcg aacgcatcac tttggaaatc
      781 gaaaaaggct tcatcaccgg tattcacggt ggctttgaag cagagtactt acgcgactac
      841 atgaagtact tcaacgatcc tgaggtttat ggcattagtc acatcggttg gggtttacaa
      901 ccacgtgcac aatggactgc catgggcttg catgaccgca atgacggtat gtgtatggat
      961 gctcgtgcct tttacggcaa ttttttattc tctaccggtc caaacacaga agtaggcggt
     1021 aagcgcaaga caccatgcca cttggacatc ccattgcgta actgcgacat ttatttggac
     1081 gataaagccg tagtattggc tggtgacgtg gtggcaccag aagaaagtcg cgcccgctaa
     1141 taatactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag
     1201 agagaatata aaaagccaga ttattaatcc ggctttttta ttattt
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