LOCUS       BBa_K1819008            2725 bp    DNA     linear       20-May-2024
DEFINITION  Promoter test circuit to analyze the kill switch efficiency
ACCESSION   BBa_K1819008
VERSION     BBa_K1819008.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..946
                     /label=GFP protein
     CDS             227..946
                     /label=BBa_E0040
     misc_feature    959..962
                     /label=conserved
     RBS             955..966
                     /label=BBa_B0034
     CDS             976..1592
                     /label=tetR
     misc_feature    1593..1626
                     /label=SsrA
     misc_feature    1633..1657
                     /label=Help:Barcodes
     CDS             973..1632
                     /label=BBa_C0040
     stem_loop       1677..1720
                     /label=stem_loop
     misc_feature    1666..1745
                     /label=BBa_B0010
     terminator      1666..1745
                     /label=BBa_B0010
     stem_loop       1761..1780
                     /label=T7 TE
     misc_feature    1787..1787
                     /label=stop
     polyA_site      1781..1794
                     /label=polya
     misc_feature    1754..1794
                     /label=BBa_B0012
     terminator      1754..1794
                     /label=BBa_B0012
     misc_feature    955..1794
                     /label=BBa_P0440
     misc_feature    1803..1821
                     /label=TetR 1
     misc_feature    1803..1856
                     /label=BBa_R0040
     promoter        1822..1827
                     /label=-35
     misc_feature    1828..1846
                     /label=TetR 2
     promoter        1845..1850
                     /label=-10
     promoter        1803..1856
                     /label=BBa_R0040
     misc_feature    1869..1872
                     /label=conserved
     RBS             1865..1876
                     /label=BBa_B0034
     CDS             1883..2557
                     /label=mrfp1
     misc_feature    2564..2588
                     /label=Help:Barcodes
     CDS             1883..2563
                     /label=BBa_E1010
     stem_loop       2608..2651
                     /label=stem_loop
     misc_feature    2597..2676
                     /label=BBa_B0010
     terminator      2597..2676
                     /label=BBa_B0010
     stem_loop       2692..2711
                     /label=T7 TE
     misc_feature    2718..2718
                     /label=stop
     polyA_site      2712..2725
                     /label=polya
     misc_feature    2685..2725
                     /label=BBa_B0012
     terminator      2685..2725
                     /label=BBa_B0012
     misc_feature    1865..2725
                     /label=BBa_I13507
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgc gtaaaggaga
      241 agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca
      301 caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa
      361 atttatttgc actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg
      421 ttatggtgtt caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa
      481 gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa
      541 ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt
      601 aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta
      661 taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt
      721 caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa
      781 tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc
      841 tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac
      901 agctgctggg attacacatg gcatggatga actatacaaa taataatact agagaaagag
      961 gagaaatact agatgtccag attagataaa agtaaagtga ttaacagcgc attagagctg
     1021 cttaatgagg tcggaatcga aggtttaaca acccgtaaac tcgcccagaa gctaggtgta
     1081 gagcagccta cattgtattg gcatgtaaaa aataagcggg ctttgctcga cgccttagcc
     1141 attgagatgt tagataggca ccatactcac ttttgccctt tagaagggga aagctggcaa
     1201 gattttttac gtaataacgc taaaagtttt agatgtgctt tactaagtca tcgcgatgga
     1261 gcaaaagtac atttaggtac acggcctaca gaaaaacagt atgaaactct cgaaaatcaa
     1321 ttagcctttt tatgccaaca aggtttttca ctagagaatg cattatatgc actcagcgct
     1381 gtggggcatt ttactttagg ttgcgtattg gaagatcaag agcatcaagt cgctaaagaa
     1441 gaaagggaaa cacctactac tgatagtatg ccgccattat tacgacaagc tatcgaatta
     1501 tttgatcacc aaggtgcaga gccagccttc ttattcggcc ttgaattgat catatgcgga
     1561 ttagaaaaac aacttaaatg tgaaagtggg tccgctgcaa acgacgaaaa ctacgcttta
     1621 gtagcttaat aacactgata gtgctagtgt agatcactac tagagccagg catcaaataa
     1681 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     1741 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag
     1801 agtccctatc agtgatagag attgacatcc ctatcagtga tagagatact gagcactact
     1861 agagaaagag gagaaatact agatggcttc ctccgaagac gttatcaaag agttcatgcg
     1921 tttcaaagtt cgtatggaag gttccgttaa cggtcacgag ttcgaaatcg aaggtgaagg
     1981 tgaaggtcgt ccgtacgaag gtacccagac cgctaaactg aaagttacca aaggtggtcc
     2041 gctgccgttc gcttgggaca tcctgtcccc gcagttccag tacggttcca aagcttacgt
     2101 taaacacccg gctgacatcc cggactacct gaaactgtcc ttcccggaag gtttcaaatg
     2161 ggaacgtgtt atgaacttcg aagacggtgg tgttgttacc gttacccagg actcctccct
     2221 gcaagacggt gagttcatct acaaagttaa actgcgtggt accaacttcc cgtccgacgg
     2281 tccggttatg cagaaaaaaa ccatgggttg ggaagcttcc accgaacgta tgtacccgga
     2341 agacggtgct ctgaaaggtg aaatcaaaat gcgtctgaaa ctgaaagacg gtggtcacta
     2401 cgacgctgaa gttaaaacca cctacatggc taaaaaaccg gttcagctgc cgggtgctta
     2461 caaaaccgac atcaaactgg acatcacctc ccacaacgaa gactacacca tcgttgaaca
     2521 gtacgaacgt gctgaaggtc gtcactccac cggtgcttaa taacgctgat agtgctagtg
     2581 tagatcgcta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     2641 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
     2701 tcgggtgggc ctttctgcgt ttata
//