LOCUS       BBa_K1820025            1209 bp    DNA     linear       01-Jun-2024
DEFINITION  p2001-native RBS-Fsp1-B0015
ACCESSION   BBa_K1820025
VERSION     BBa_K1820025.1  
FEATURES             Location/Qualifiers
     promoter        71..99
                     /label=Leftward Promoter
     misc_feature    71..76
                     /label=Leftward -10
     misc_feature    88..115
                     /label=O1
     misc_feature    94..99
                     /label=Leftward -35
     promoter        118..145
                     /label=Rightward Promoter
     misc_feature    118..123
                     /label=Rightward -35
     misc_feature    140..145
                     /label=Rightward -10
     misc_feature    152..180
                     /label=O2
     misc_feature    153..153
                     /label=Transcriptional Start Site
     RBS             304..312
                     /label=Native RBS
     promoter        1..314
                     /label=BBa_K1820003
     CDS             323..1069
                     /label=FspI
     misc_feature    1069..1072
                     /label=stop codon
     CDS             323..1072
                     /label=BBa_K1820022
     stem_loop       1092..1135
                     /label=stem_loop
     misc_feature    1081..1160
                     /label=BBa_B0010
     terminator      1081..1160
                     /label=BBa_B0010
     stem_loop       1176..1195
                     /label=T7 TE
     misc_feature    1202..1202
                     /label=stop
     polyA_site      1196..1209
                     /label=polya
     misc_feature    1169..1209
                     /label=BBa_B0012
     terminator      1169..1209
                     /label=BBa_B0012
     terminator      1081..1209
                     /label=BBa_B0015
     misc_feature    323..1209
                     /label=BBa_K1820023
ORIGIN
        1 cctgtcttaa tcctcggtcg ttcattattt tctttaatct catttgagag ttttcttttt
       61 tcatgaactt attataaaaa catttataaa aaagttcaaa ctttttatac tttttccttg
      121 acaattattt tccaaatgat ataattaaat cataaagttc aaaacatttg aactttatat
      181 caaacaagcg aacaatcatg gagcattcag tacggcagac ggaacaggct caaatgacgg
      241 tacacgacgt atccaccgcg acgtaagtag caagtttggc aaataaaaag ccccagaggg
      301 gcagaaagga tgtatactag aggtgttgac aaacaacgag attgaaagat taaggcaagc
      361 cattatcgcg accattgcat ctcctgtaat tggctcgata gaagattata catgggaagc
      421 aatttttcat tatgttaagg atattccttt atcagatccc gctctgggac gcagcaagct
      481 tctctatgat gctgttgacg tagttactaa aactggttgg tcactcaaat ccctccaatt
      541 gaagagcctt aactttaaaa gcccattttt atttgttatt cagagagcag atatccttaa
      601 gaagtctgtc cagctgggtt ttcctggtct gactgagcaa tcttcgccgg atgagcttgg
      661 agcagccatt atccaacatt ggaatgagaa gattattttg agtcaggcag cacaaagcgt
      721 tgtaaatagt tatgaaggca tattactgaa aactatcaaa ggttacgagt atatctattg
      781 tgagtttcca ctcgatcctc ttgatccaag cacgttttct tgggcttgga cggtggacaa
      841 aactactggc ggtgcagggg tagggctaca aggtagcatt gtgggcaaaa cagaattagt
      901 gtggtataaa aatcagaaac aacttttcag agctaggact attcccgcac aagcggttcg
      961 tattacagtt gaaagaactc gtcttactct tgatcgatat gtaaagacag ttatctttgc
     1021 tttgcaagat caaatcaaca tgcagttttc tgagaatgag cctgaagaat agtactagag
     1081 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     1141 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
     1201 gcgtttata
//