Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: MD. RISAT UL HAQUE, Daniel J. Leirer
Date created: 2009-08-12 11:00:00
Date modified: 2015-05-08 01:11:06
TetR regulated by CI operator (RBS+, Term-) with LVA tag
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K182005_sequence (Version 1) |
Description
This is a part using the biobrick BBa_K182004 as the upstream part and BBa_S03518 as the downstream part.BBa_K182004 is a cI regulated promoter. The promoter has two binding sites for the cI repressor and is repressed when it binds.It is identical to BBa_R0051 apart from a point mutation in the -35 promoter binding region(15th base is C instead of T).
BBa_S03518 is a TetR protein with a Ribosome Binding site upstream and an LVA tag attached to its end. TetR binds to TetR regulated promoters such as BBa_R0040. aTc (anhydrotetracycline) binds to TetR causing it to be released from the promoter and reintroducing transcription.
This part comes without a terminator.
Notes
BBa_K182004 was used as an upstream part and BBa_S03518 as a downstream part.Source
BBa_K182004 , BBa_S03518| Access | Instance | Definition |
| public public | BBa_K182004 BBa_S03518 | BBa_K182004 BBa_S03518 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K182004 BBa_S03518 | 1,49 58,760 | BBa_K182004 BBa_S03518 |