LOCUS       BBa_K182005              760 bp    DNA     linear       04-May-2024
DEFINITION  TetR  regulated by  CI operator  (RBS+, Term-) with LVA tag
ACCESSION   BBa_K182005
VERSION     BBa_K182005.1  
FEATURES             Location/Qualifiers
     misc_feature    1..49
                     /label=BBa_K182004
     promoter        15..20
                     /label=-35
     promoter        37..43
                     /label=-10
     misc_feature    1..19
                     /label=OR2
     misc_feature    25..42
                     /label=OR1
     misc_feature    15..15
                     /label=Point Mutation
     promoter        1..49
                     /label=BBa_K182004
     misc_feature    62..65
                     /label=conserved
     RBS             58..69
                     /label=BBa_B0034
     CDS             79..695
                     /label=tetR
     misc_feature    696..729
                     /label=SsrA
     misc_feature    736..760
                     /label=Help:Barcodes
     CDS             76..760
                     /label=BBa_C0040
     misc_feature    58..760
                     /label=BBa_S03518
ORIGIN
        1 taacaccgtg cgtgctgact attttacctc tggcggtgat aatggttgct actagagaaa
       61 gaggagaaat actagatgtc cagattagat aaaagtaaag tgattaacag cgcattagag
      121 ctgcttaatg aggtcggaat cgaaggttta acaacccgta aactcgccca gaagctaggt
      181 gtagagcagc ctacattgta ttggcatgta aaaaataagc gggctttgct cgacgcctta
      241 gccattgaga tgttagatag gcaccatact cacttttgcc ctttagaagg ggaaagctgg
      301 caagattttt tacgtaataa cgctaaaagt tttagatgtg ctttactaag tcatcgcgat
      361 ggagcaaaag tacatttagg tacacggcct acagaaaaac agtatgaaac tctcgaaaat
      421 caattagcct ttttatgcca acaaggtttt tcactagaga atgcattata tgcactcagc
      481 gctgtggggc attttacttt aggttgcgta ttggaagatc aagagcatca agtcgctaaa
      541 gaagaaaggg aaacacctac tactgatagt atgccgccat tattacgaca agctatcgaa
      601 ttatttgatc accaaggtgc agagccagcc ttcttattcg gccttgaatt gatcatatgc
      661 ggattagaaa aacaacttaa atgtgaaagt gggtccgctg caaacgacga aaactacgct
      721 ttagtagctt aataacactg atagtgctag tgtagatcac
//