LOCUS       BBa_K182200             1651 bp    DNA     linear       17-May-2024
DEFINITION  Constitutively expressed LuxR/LuxI operon
ACCESSION   BBa_K182200
VERSION     BBa_K182200.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23107
     RBS             44..58
                     /label=RBS-1\Strong
     RBS             51..55
                     /label=RBS
     misc_feature    44..58
                     /label=BBa_B0030
     RBS             44..58
                     /label=BBa_B0030
     misc_feature    644..675
                     /label=LVA
     CDS             65..643
                     /label=luxI
     misc_feature    65..682
                     /label=BBa_C0061
     misc_feature    683..707
                     /label=Help:Barcodes
     CDS             65..682
                     /label=BBa_C0061
     misc_feature    44..707
                     /label=BBa_K081008
     misc_feature    720..723
                     /label=conserved
     RBS             716..727
                     /label=BBa_B0034
     misc_feature    734..735
                     /label=prefix
     misc_feature    907..907
                     /label=T
     misc_feature    1225..1225
                     /label=A
     CDS             734..1483
                     /label=luxR
     misc_feature    734..1489
                     /label=BBa_C0062
     misc_feature    1490..1514
                     /label=Help:Barcodes
     CDS             734..1489
                     /label=BBa_C0062
     stem_loop       1534..1577
                     /label=stem_loop
     misc_feature    1523..1602
                     /label=BBa_B0010
     terminator      1523..1602
                     /label=BBa_B0010
     stem_loop       1618..1637
                     /label=T7 TE
     misc_feature    1644..1644
                     /label=stop
     polyA_site      1638..1651
                     /label=polya
     misc_feature    1611..1651
                     /label=BBa_B0012
     terminator      1611..1651
                     /label=BBa_B0012
     misc_feature    716..1651
                     /label=BBa_I0462
ORIGIN
        1 tttacggcta gctcagccct aggtattatg ctagctacta gagattaaag aggagaaata
       61 ctagatgact ataatgataa aaaaatcgga ttttttggca attccatcgg aggagtataa
      121 aggtattcta agtcttcgtt atcaagtgtt taagcaaaga cttgagtggg acttagttgt
      181 agaaaataac cttgaatcag atgagtatga taactcaaat gcagaatata tttatgcttg
      241 tgatgatact gaaaatgtaa gtggatgctg gcgtttatta cctacaacag gtgattatat
      301 gctgaaaagt gtttttcctg aattgcttgg tcaacagagt gctcccaaag atcctaatat
      361 agtcgaatta agtcgttttg ctgtaggtaa aaatagctca aagataaata actctgctag
      421 tgaaattaca atgaaactat ttgaagctat atataaacac gctgttagtc aaggtattac
      481 agaatatgta acagtaacat caacagcaat agagcgattt ttaaagcgta ttaaagttcc
      541 ttgtcatcgt attggagaca aagaaattca tgtattaggt gatactaaat cggttgtatt
      601 gtctatgcct attaatgaac agtttaaaaa agcagtctta aatgctgcaa acgacgaaaa
      661 ctacgcttta gtagcttaat aactctgata gtgctagtgt agatctctac tagagaaaga
      721 ggagaaatac tagatgaaaa acataaatgc cgacgacaca tacagaataa ttaataaaat
      781 taaagcttgt agaagcaata atgatattaa tcaatgctta tctgatatga ctaaaatggt
      841 acattgtgaa tattatttac tcgcgatcat ttatcctcat tctatggtta aatctgatat
      901 ttcaatccta gataattacc ctaaaaaatg gaggcaatat tatgatgacg ctaatttaat
      961 aaaatatgat cctatagtag attattctaa ctccaatcat tcaccaatta attggaatat
     1021 atttgaaaac aatgctgtaa ataaaaaatc tccaaatgta attaaagaag cgaaaacatc
     1081 aggtcttatc actgggttta gtttccctat tcatacggct aacaatggct tcggaatgct
     1141 tagttttgca cattcagaaa aagacaacta tatagatagt ttatttttac atgcgtgtat
     1201 gaacatacca ttaattgttc cttctctagt tgataattat cgaaaaataa atatagcaaa
     1261 taataaatca aacaacgatt taaccaaaag agaaaaagaa tgtttagcgt gggcatgcga
     1321 aggaaaaagc tcttgggata tttcaaaaat attaggttgc agtgagcgta ctgtcacttt
     1381 ccatttaacc aatgcgcaaa tgaaactcaa tacaacaaac cgctgccaaa gtatttctaa
     1441 agcaatttta acaggagcaa ttgattgccc atactttaaa aattaataac actgatagtg
     1501 ctagtgtaga tcactactag agccaggcat caaataaaac gaaaggctca gtcgaaagac
     1561 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
     1621 tcaccttcgg gtgggccttt ctgcgtttat a
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