LOCUS       BBa_K1833004            1076 bp    DNA     linear       11-Jun-2024
DEFINITION  lac promoter -> RpoA-N-terminus fused to Gal4
ACCESSION   BBa_K1833004
VERSION     BBa_K1833004.1  
FEATURES             Location/Qualifiers
     misc_feature    37..57
                     /label=lac operator
     promoter        1..93
                     /label=lac promoter
     RBS             112..119
                     /label=rbs
     misc_feature    125..128
                     /label=start
     misc_feature    129..145
                     /label=6xHIS tag
     CDS             146..847
                     /label=RpoA amino acids 2-235
     CDS             848..856
                     /label=linker
     CDS             857..976
                     /label=Gal4 amino acids 58-97
     misc_feature    977..982
                     /label=stop
     stem_loop       983..1070
                     /label=terminator
ORIGIN
        1 tttacacttt atgcttccgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
       61 cacaggaaac agctatgacc atgattacgg attcactgga actcgagacc aaagagagga
      121 cacaatgcat catcatcatc accatcaagg ttcagtgacg gagtttttaa aaccgcgcct
      181 ggtggatatc gagcaagttt cttcgactca tgcgaaagtg acactggagc cgctggaacg
      241 cggctttggt cacaccctgg ggaatgctct gcgtcggatc ctgctgtcgt caatgcccgg
      301 gtgtgcggtt acggaagtgg aaatcgatgg cgttcttcac gagtacagca ccaaagaagg
      361 cgttcaggag gacatcctcg aaatcctgct caatctcaaa ggactggcag tgcgcgttca
      421 gggtaaagat gaagtgattt taacccttaa taaatcgggc attggcccgg taacagcggc
      481 ggatatcacg catgacggtg atgtcgaaat cgtaaagccg cagcatgtaa tttgtcacct
      541 cacagatgaa aacgcctcaa tctctatgcg catcaaagta cagcggggtc gtggttacgt
      601 cccggcgagc actcgtatcc atagcgaaga ggacgaacgt ccgattgggc gtctgctggt
      661 cgatgcgtgt tattcacctg tcgaacgcat cgcctacaat gtagaggcgg cgcgtgtaga
      721 acaacgcacg gatctggaca aactggtcat cgaaatggaa acaaatggaa ccatcgatcc
      781 agaagaggcc attcgtcgtg ccgcgacgat cttggcggaa caacttgaag cttttgtgga
      841 cctgcgggcg gctgccgaat cacgtctgga acgcctggaa caactgttcc tgctcatttt
      901 tccgcgcgag gaccttgaca tgatcctgaa aatggactcc ctccaggata tcaaggcact
      961 tcttacaggc ctgttctaat agtactagag ccaggcatca aataaaacga aaggctcagt
     1021 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc gtcgac
//