Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Konstantin Borisov
Date created: 2015-09-17 11:00:00
Date modified: 2015-09-17 11:13:57
T7 promoter -> 434 cI N terminus with P22 repressor specificity
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K1833009_sequence (Version 1) |
Description
This part produces the DNA binding domain of the phage 434 cI repressor, with mutations that change the specificity of the DNA to which the protein binds. This protein domain was used as in "A two-hybrid system based on chimeric operator recognition for studying protein homo/heterodimerization in Escherichia coli" (G. Di Lallo, L. Castagnoli, P. Ghelardini and L. Paolozzi; Microbiology (2001), 147, 1651???1656). For more information on the uses of this part, visit 2015.igem.org/Team:Pitt/Protease/Project.Notes
This part was designed with a T7 promoter so the protein could be overexpressed.Source
"A two-hybrid system based on chimeric operator recognition for studying protein homo/heterodimerization in Escherichia coli" (G. Di Lallo, L. Castagnoli, P. Ghelardini and L. Paolozzi; Microbiology (2001), 147, 1651???1656) described the use of this protein domain.| Sequence Annotation | Location | Component / Role(s) |
| pT7 rbs P22-like 434 cI N-terminus T7 terminator | 1,20 24,29 33,317 318,358 | feature/promoter promoter ribosome_entry_site feature/rbs feature/cds CDS feature/stem_loop stem_loop |