LOCUS       BBa_K1833011             632 bp    DNA     linear       29-May-2024
DEFINITION  lac promoter -> Zinc finger 225 binding domain fused to 
            immunogenic MUC1 peptide
ACCESSION   BBa_K1833011
VERSION     BBa_K1833011.1  
FEATURES             Location/Qualifiers
     promoter        1..93
                     /label=pLac
     misc_feature    37..57
                     /label=lacI binding site
     RBS             112..119
                     /label=rbs
     misc_feature    125..127
                     /label=start
     CDS             128..247
                     /label=MUC1 immunogenic peptide (x2)
     CDS             248..256
                     /label=linker
     CDS             257..538
                     /label=Zif268 binding domain
     misc_feature    539..544
                     /label=stop
     stem_loop       545..632
                     /label=terminator
ORIGIN
        1 tttacacttt atgcttccgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
       61 cacaggaaac agctatgacc atgattacgg attcactgga actcgagacc aaagagagga
      121 cacaatggct ccggacaccc ggccagcgcc gggcagcacc gctcctccgg cacacggtgt
      181 gacaagcgcg ccggataccc gcccagcccc gggatctaca gcacctccag cccacggtgt
      241 gacgagtgca gcagcgccct ccaagacgcc gccgcacgaa cgtccatacg cctgcccggt
      301 cgaaagttgt gatcgtcggt ttagtcgttc cgatgagctt acccgtcaca tccgtattca
      361 tacaggccag aaaccgttcc agtgccgtat ttgcatgcgc aacttttcgc gcagtgacca
      421 tttaactact cacatccgca cgcatacggg cgagaagccg ttcgcctgcg acatttgtgg
      481 ccgcaagttc gcgcgcagcg acgaacgcaa gcgtcacacc aaaatccacc tgcggcagta
      541 atagtactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
      601 gttttatctg ttgtttgtcg gtgaacgctc tc
//