BBa_K1835000 1 BBa_K1835000 Empty gRNA Scaffold 2015-09-16T11:00:00Z 2015-09-17T03:04:36Z The part has been in use in our lab originally from the Luciano Marrafini Lab originating from S. Peumoniae. Using the restriction enzyme BsaI, an opening will be made to insert annealed oligonucleotides that can code your gRNA. false false _2261_ 26097 26097 9 false The BsaI sites were devised with enough space to avoid interference and designed to cut themselves out from the produced backbone. false Anthony Ciesla annotation2467042 1 gRNA Oligos Binding Site range2467042 1 53 56 annotation2467020 1 Repeat Loop range2467020 1 86 122 annotation2467053 1 gRNA Oligos Binding Site range2467053 1 82 85 annotation2467017 1 Repeat range2467017 1 20 55 BBa_K1835000_sequence 1 agacaaaaatagtctacgaggttttagagctatgctgttttgaatggtcccaaaactgagaccagtctcggaagctcaaaggtctcgttttagagctatgctgttttgaatggtcccaaaacttcagcacactgaga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z