BBa_K1835000
1
BBa_K1835000
Empty gRNA Scaffold
2015-09-16T11:00:00Z
2015-09-17T03:04:36Z
The part has been in use in our lab originally from the Luciano Marrafini Lab originating from S. Peumoniae.
Using the restriction enzyme BsaI, an opening will be made to insert annealed oligonucleotides that can code your gRNA.
false
false
_2261_
26097
26097
9
false
The BsaI sites were devised with enough space to avoid interference and designed to cut themselves out from the produced backbone.
false
Anthony Ciesla
annotation2467042
1
gRNA Oligos Binding Site
range2467042
1
53
56
annotation2467020
1
Repeat Loop
range2467020
1
86
122
annotation2467053
1
gRNA Oligos Binding Site
range2467053
1
82
85
annotation2467017
1
Repeat
range2467017
1
20
55
BBa_K1835000_sequence
1
agacaaaaatagtctacgaggttttagagctatgctgttttgaatggtcccaaaactgagaccagtctcggaagctcaaaggtctcgttttagagctatgctgttttgaatggtcccaaaacttcagcacactgaga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z