LOCUS       BBa_K1846007            1696 bp    DNA     linear       18-May-2024
DEFINITION  Bacteriophage lambda tail fibre assembly (tfa) under TetR 
            regulation
ACCESSION   BBa_K1846007
VERSION     BBa_K1846007.1  
FEATURES             Location/Qualifiers
     promoter        4..9
                     /label=-35
     promoter        27..32
                     /label=-10
     misc_feature    1..38
                     /label=P(Cat)
     promoter        1..38
                     /label=BBa_I14033
     misc_feature    53..56
                     /label=conserved
     RBS             47..60
                     /label=BBa_B0031
     CDS             70..686
                     /label=tetR
     misc_feature    687..720
                     /label=SsrA
     misc_feature    727..751
                     /label=Help:Barcodes
     CDS             67..726
                     /label=BBa_C0040
     stem_loop       771..814
                     /label=stem_loop
     misc_feature    760..839
                     /label=BBa_B0010
     terminator      760..839
                     /label=BBa_B0010
     stem_loop       855..874
                     /label=T7 TE
     misc_feature    881..881
                     /label=stop
     polyA_site      875..888
                     /label=polya
     misc_feature    848..888
                     /label=BBa_B0012
     terminator      848..888
                     /label=BBa_B0012
     misc_feature    47..888
                     /label=BBa_P0140
     misc_feature    897..915
                     /label=TetR 1
     misc_feature    897..950
                     /label=BBa_R0040
     promoter        916..921
                     /label=-35
     misc_feature    922..940
                     /label=TetR 2
     promoter        939..944
                     /label=-10
     promoter        897..950
                     /label=BBa_R0040
     misc_feature    951..969
                     /label=TetR 1
     misc_feature    951..1004
                     /label=BBa_R0040
     promoter        970..975
                     /label=-35
     misc_feature    976..994
                     /label=TetR 2
     promoter        993..998
                     /label=-10
     promoter        951..976
                     /label=BBa_R0040
     misc_feature    951..1004
                     /label=BBa_R0040
     misc_feature    951..969
                     /label=TetR 1
     promoter        970..975
                     /label=-35
     misc_feature    976..994
                     /label=TetR 2
     promoter        993..998
                     /label=-10
     misc_feature    1017..1020
                     /label=conserved
     misc_feature    1013..1024
                     /label=BBa_B0034
     misc_feature    951..976
                     /label=BBa_S05276
     misc_feature    981..984
                     /label=conserved
     RBS             977..988
                     /label=BBa_B0034
     misc_feature    993..996
                     /label=conserved
     RBS             989..994
                     /label=BBa_B0034
     misc_feature    993..996
                     /label=conserved
     misc_feature    989..1000
                     /label=BBa_B0034
     misc_feature    1007..1591
                     /label=BBa_K1846002
     misc_feature    989..994
                     /label=BBa_S05278
     CDS             995..1579
                     /label=BBa_K1846002
     CDS             1580..1616
                     /label=BBa_K1846002
     misc_feature    1580..2164
                     /label=BBa_K1846002
     misc_feature    2173..2252
                     /label=BBa_B0010
     stem_loop       2184..2227
                     /label=stem_loop
     misc_feature    1580..1616
                     /label=BBa_S05279
     stem_loop       1628..1671
                     /label=stem_loop
     misc_feature    1617..1696
                     /label=BBa_B0010
     terminator      1617..1696
                     /label=BBa_B0010
     misc_feature    897..1696
                     /label=BBa_K1846001
ORIGIN
        1 ggcacgtaag aggttccaac tttcaccata atgaaacata ctagagtcac acaggaaacc
       61 tactagatgt ccagattaga taaaagtaaa gtgattaaca gcgcattaga gctgcttaat
      121 gaggtcggaa tcgaaggttt aacaacccgt aaactcgccc agaagctagg tgtagagcag
      181 cctacattgt attggcatgt aaaaaataag cgggctttgc tcgacgcctt agccattgag
      241 atgttagata ggcaccatac tcacttttgc cctttagaag gggaaagctg gcaagatttt
      301 ttacgtaata acgctaaaag ttttagatgt gctttactaa gtcatcgcga tggagcaaaa
      361 gtacatttag gtacacggcc tacagaaaaa cagtatgaaa ctctcgaaaa tcaattagcc
      421 tttttatgcc aacaaggttt ttcactagag aatgcattat atgcactcag cgctgtgggg
      481 cattttactt taggttgcgt attggaagat caagagcatc aagtcgctaa agaagaaagg
      541 gaaacaccta ctactgatag tatgccgcca ttattacgac aagctatcga attatttgat
      601 caccaaggtg cagagccagc cttcttattc ggccttgaat tgatcatatg cggattagaa
      661 aaacaactta aatgtgaaag tgggtccgct gcaaacgacg aaaactacgc tttagtagct
      721 taataacact gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa
      781 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
      841 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagtccc
      901 tatcagtgat agagattgac atccctatca gtgatagaga tactgagcac gatgtcaaag
      961 aatacaagca ttgactaaag aggagaaaat acaaatggcg ttccgtatga gtgaacaacc
     1021 acgtaccatt aaaatttata atctgctggc cggtactaat gaatttattg gtgaaggtga
     1081 cgcatatatt ccgcctcata ccggcctgcc tgcaaacagt accgatattg caccgccaga
     1141 tattccggct ggctttgtgg ctgttttcaa cagtgatgag gcatcgtggc atctcgttga
     1201 agaccatcgt ggtaaaaccg tctatgacgt ggcttccggc gacgcgttat ttatttctga
     1261 actcggtccg ttaccggaaa attttacctg gttatcgccg ggtggggaat atcagaagtg
     1321 gaacggcaca gcctgggtga aggatacgga agcagaaaaa ctgttccgta tccgtgaggc
     1381 ggaagaaaca aaaaaaagcc tgatgcaagt agccagtgag catattgcgc cgcttcagga
     1441 tgcggcggat ctggaaattg caacgaagga agaaacctcg ttgctggaag cctggaagaa
     1501 gtatcgtgtg ttgctgaacc gtgttgatac atcaactgca cctgatattg agtggcctgc
     1561 tgtccctgtt atggagtaat aacgctgata ctgatagtgc tagtagtgta gatcgcccag
     1621 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     1681 gtcggtgaac gctctc
//