BBa_C0040
1
tetR
tetracycline repressor from transposon Tn10 (+LVA)
2003-01-31T12:00:00Z
2015-08-31T04:07:23Z
Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999)
Released HQ 2013
Coding region for the TetR protein without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.</P>
false
true
_1_
0
24
7
In stock
false
References (unparsed) here: <p>Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). </P> <p> Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 </p> <P> References (unparsed) here: <p>Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). </P> <p> Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 </p> <P>BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA. <P>
true
June Rhee, Connie Tao, Ty Thomson, Louis Waldman.
annotation23330
1
SsrA
range23330
1
621
654
annotation2213989
1
Help:Barcodes
range2213989
1
661
685
annotation23329
1
tetR
range23329
1
4
620
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_K1846001
1
tfa+
Bacteriophage lambda tail fibre assembly protein circuit
2015-08-25T11:00:00Z
2015-09-20T01:26:15Z
...
The tfa (tail fibre assembly) protein of bacteriophage Lambda assists in the assembly of the stf (short tail fibre) protein into a functional tail fibre. This part provides the gene sequence for tfa, together with a TetR repressible promoter (TetO, available separately as BioBrick part BBa_R0040), ribosome binding site (available separately as part BBa_B0034) and an rrNB T1 terminator (available separately as BioBrick part BBa_B0010).
false
false
_2272_
24377
27305
9
false
...
false
Luba Prout
component2439269
1
BBa_R0040
component2439277
1
BBa_S05278
component2439279
1
BBa_K1846002
component2439275
1
BBa_B0034
component2439272
1
BBa_S05276
component2439274
1
BBa_B0034
component2439278
1
BBa_K1846002
component2439281
1
BBa_B0010
component2439264
1
BBa_R0040
component2439280
1
BBa_S05279
annotation2439281
1
BBa_B0010
range2439281
1
721
800
annotation2439264
1
BBa_R0040
range2439264
1
1
54
annotation2439274
1
BBa_B0034
range2439274
1
81
92
annotation2439278
1
BBa_K1846002
range2439278
1
99
683
annotation2439279
1
BBa_K1846002
range2439279
1
684
720
annotation2439269
1
BBa_R0040
range2439269
1
55
80
annotation2439275
1
BBa_B0034
range2439275
1
93
98
annotation2439277
1
BBa_S05278
range2439277
1
93
98
annotation2439272
1
BBa_S05276
range2439272
1
55
80
annotation2439280
1
BBa_S05279
range2439280
1
684
720
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K1846002
1
tfa
Bacteriophage lambda tail fibre assembly (tfa) protein
2015-08-25T11:00:00Z
2015-09-20T01:20:28Z
The sequence has been adapted from the genomic sequence of bacteriophage Lambda.
The tfa (tail fibre assembly) protein of bacteriophage Lambda assists in the assembly of the stf (short tail fibre) protein into a functional tail fibre.
false
false
_2272_
24377
27305
9
false
Sequence was optimised to remove illegal restriction sites.
false
Luba Prout
BBa_S05276
1
BBa_S05276
R0040:B0034
2015-08-27T11:00:00Z
2015-08-28T03:23:28Z
false
false
_9_
27305
27305
9
false
false
Barbara Steijl
annotation2439172
1
TetR 1
range2439172
1
1
19
annotation2439175
1
-10
range2439175
1
43
48
annotation2439176
1
conserved
range2439176
1
67
70
annotation2439174
1
TetR 2
range2439174
1
26
44
annotation2439173
1
-35
range2439173
1
20
25
annotation2439171
1
BBa_R0040
range2439171
1
1
54
annotation2439177
1
BBa_B0034
range2439177
1
63
74
BBa_R0040
1
p(tetR)
TetR repressible promoter
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210.
Released HQ 2013
Sequence for pTet inverting regulator driven by the TetR protein.</P>
false
true
_1_
0
24
7
In stock
false
<P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P>
true
June Rhee, Connie Tao, Ty Thomson, Louis Waldman
annotation1986784
1
BBa_R0040
range1986784
1
1
54
annotation1986785
1
-35
range1986785
1
20
25
annotation1986783
1
TetR 1
range1986783
1
1
19
annotation1986787
1
-10
range1986787
1
43
48
annotation1986786
1
TetR 2
range1986786
1
26
44
BBa_I14033
1
Cat
P(Cat)
2004-08-03T11:00:00Z
2015-08-31T04:07:37Z
Plasmid pACYC184
Released HQ 2013
Constitutive Promoter, Medium Transcription
false
false
_4_
0
171
7
In stock
false
true
Vikram Vijayan, Allen Hsu, Lawrence Fomundam
annotation1026172
1
-35
range1026172
1
4
9
annotation1026174
1
P(Cat)
range1026174
1
1
38
annotation1026173
1
-10
range1026173
1
27
32
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_P0140
1
BBa_P0140
PoPS -> TetR [S0163]
2004-04-24T11:00:00Z
2015-05-08T01:14:10Z
Released HQ 2013
Protein generator converting TIPS to the protein TetR. Used as the input section for Quad Part Inverter Q01140.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
component944448
1
BBa_B0010
component944442
1
BBa_C0040
component944432
1
BBa_B0031
component944458
1
BBa_B0012
annotation944458
1
BBa_B0012
range944458
1
802
842
annotation944448
1
BBa_B0010
range944448
1
714
793
annotation944432
1
BBa_B0031
range944432
1
1
14
annotation944442
1
BBa_C0040
range944442
1
21
680
BBa_S05278
1
BBa_S05278
B0034:K1846002
2015-08-27T11:00:00Z
2015-08-28T03:51:09Z
false
false
_9_
27305
27305
9
false
false
Barbara Steijl
annotation2439242
1
BBa_B0034
range2439242
1
1
12
annotation2439243
1
BBa_K1846002
range2439243
1
19
603
annotation2439241
1
conserved
range2439241
1
5
8
BBa_B0031
1
BBa_B0031
RBS.2 (weak) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Medium RBS based on Ron Weiss thesis. Strength considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
<P> <P>Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-1" in figure 4-14 of thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Cho</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23316
1
conserved
range23316
1
7
10
BBa_S05279
1
BBa_S05279
K1846002:B0010
2015-08-27T11:00:00Z
2015-08-28T03:52:59Z
false
false
_9_
27305
27305
9
false
false
Barbara Steijl
annotation2439262
1
BBa_B0010
range2439262
1
594
673
annotation2439261
1
BBa_K1846002
range2439261
1
1
585
annotation2439263
1
stem_loop
range2439263
1
605
648
BBa_K1846007
1
TetR+tfa+
Bacteriophage lambda tail fibre assembly (tfa) under TetR regulation
2015-09-16T11:00:00Z
2015-09-20T01:47:20Z
...
A circuit controlling the production of the tail fibre assembly (tfa) protein of bacteriophage lambda. The tfa gene operates under a TetR repressible promoter, while a second circuit produces TetR (tetracycline repressor) under control of a P(Cat) promoter.
false
false
_2272_
24377
27305
9
true
...
false
Luba Prout
component2465868
1
BBa_K1846001
component2465848
1
BBa_P0140
component2465835
1
BBa_I14033
annotation2465835
1
BBa_I14033
range2465835
1
1
38
annotation2465868
1
BBa_K1846001
range2465868
1
897
1696
annotation2465848
1
BBa_P0140
range2465848
1
47
888
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K1846001_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcacgatgtcaaagaatacaagcattgactaaagaggagaaaatacaaatggcgttccgtatgagtgaacaaccacgtaccattaaaatttataatctgctggccggtactaatgaatttattggtgaaggtgacgcatatattccgcctcataccggcctgcctgcaaacagtaccgatattgcaccgccagatattccggctggctttgtggctgttttcaacagtgatgaggcatcgtggcatctcgttgaagaccatcgtggtaaaaccgtctatgacgtggcttccggcgacgcgttatttatttctgaactcggtccgttaccggaaaattttacctggttatcgccgggtggggaatatcagaagtggaacggcacagcctgggtgaaggatacggaagcagaaaaactgttccgtatccgtgaggcggaagaaacaaaaaaaagcctgatgcaagtagccagtgagcatattgcgccgcttcaggatgcggcggatctggaaattgcaacgaaggaagaaacctcgttgctggaagcctggaagaagtatcgtgtgttgctgaaccgtgttgatacatcaactgcacctgatattgagtggcctgctgtccctgttatggagtaataacgctgatactgatagtgctagtagtgtagatcgcccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_P0140_sequence
1
tcacacaggaaacctactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_I14033_sequence
1
ggcacgtaagaggttccaactttcaccataatgaaaca
BBa_K1846007_sequence
1
ggcacgtaagaggttccaactttcaccataatgaaacatactagagtcacacaggaaacctactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcacgatgtcaaagaatacaagcattgactaaagaggagaaaatacaaatggcgttccgtatgagtgaacaaccacgtaccattaaaatttataatctgctggccggtactaatgaatttattggtgaaggtgacgcatatattccgcctcataccggcctgcctgcaaacagtaccgatattgcaccgccagatattccggctggctttgtggctgttttcaacagtgatgaggcatcgtggcatctcgttgaagaccatcgtggtaaaaccgtctatgacgtggcttccggcgacgcgttatttatttctgaactcggtccgttaccggaaaattttacctggttatcgccgggtggggaatatcagaagtggaacggcacagcctgggtgaaggatacggaagcagaaaaactgttccgtatccgtgaggcggaagaaacaaaaaaaagcctgatgcaagtagccagtgagcatattgcgccgcttcaggatgcggcggatctggaaattgcaacgaaggaagaaacctcgttgctggaagcctggaagaagtatcgtgtgttgctgaaccgtgttgatacatcaactgcacctgatattgagtggcctgctgtccctgttatggagtaataacgctgatactgatagtgctagtagtgtagatcgcccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_S05276_sequence
1
gatgtcaaagaatacaagcattgact
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_S05278_sequence
1
atacaa
BBa_R0040_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
BBa_S05279_sequence
1
taacgctgatactgatagtgctagtagtgtagatcgc
BBa_C0040_sequence
1
atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
BBa_B0031_sequence
1
tcacacaggaaacc
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K1846002_sequence
1
atggcgttccgtatgagtgaacaaccacgtaccattaaaatttataatctgctggccggtactaatgaatttattggtgaaggtgacgcatatattccgcctcataccggcctgcctgcaaacagtaccgatattgcaccgccagatattccggctggctttgtggctgttttcaacagtgatgaggcatcgtggcatctcgttgaagaccatcgtggtaaaaccgtctatgacgtggcttccggcgacgcgttatttatttctgaactcggtccgttaccggaaaattttacctggttatcgccgggtggggaatatcagaagtggaacggcacagcctgggtgaaggatacggaagcagaaaaactgttccgtatccgtgaggcggaagaaacaaaaaaaagcctgatgcaagtagccagtgagcatattgcgccgcttcaggatgcggcggatctggaaattgcaacgaaggaagaaacctcgttgctggaagcctggaagaagtatcgtgtgttgctgaaccgtgttgatacatcaactgcacctgatattgagtggcctgctgtccctgttatggagtaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z