LOCUS       BBa_K1847015            2810 bp    DNA     linear       09-May-2024
DEFINITION  promoter-RBS-INP-annexin V-RFP
ACCESSION   BBa_K1847015
VERSION     BBa_K1847015.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=J23114
     RBS             44..55
                     /label=B0034
     CDS             62..994
                     /label=INP
     misc_feature    995..1045
                     /label=linker sequence
     CDS             1046..2008
                     /label=Annexin V
     misc_feature    2020..2060
                     /label=B0012
     promoter        2069..2103
                     /label=J23114
     RBS             2112..2123
                     /label=B0034
     CDS             2130..2807
                     /label=RFP
ORIGIN
        1 tttatggcta gctcagtcct aggtacaatg ctagctacta gagaaagagg agaaatacta
       61 gatgggatct atgaccctgg ataaagcgct ggtgctgcgc acttgtgcca ataatatggc
      121 cgaccattgt ggcctgatct ggcctgcttc tggtacagtt gaaagccgct attggcagtc
      181 tacacgtcgt cacgaaaacg ggctggttgg gctgctgtgg ggtgccggaa catccgcctt
      241 tctgagcgtt catgccgatg ctcgttggat tgtttgtgag gttgctgttg ccgacatcat
      301 tagcctggaa gaaccgggta tggtgaaatt tcctcgtgct gaagtggtcc atgttggtga
      361 tcgtattagc gcctcccatt tcatttcagc tcgtcaggct gatccagcaa gcacatcaac
      421 aagcacctct accagtacac tgacaccaat gccaaccgct atccctactc ctatgccggc
      481 tgtagcctct gttactctgc ctgtggccga acaagctcgc cacgaagtct ttgacgttgc
      541 ctctgtgtct gctgctgctg ctcctgttaa cactctgcct gttaccacgc ctcaaaatct
      601 gcaaacggcg acctatggct caacactgag tggtgataac cattctcgtc tgattgccgg
      661 gtatggctct aacgaaaccg ccggaaacca ttctgatctg atcggtggtc acgattgtac
      721 gctgatggca ggtgatcaat ctcgtctgac cgctggcaaa aattccgttc tgaccgctgg
      781 ggcacgttct aaactgatcg ggagcgaagg ctctactctg tccgccggag aagattctac
      841 cctgatcttt cgtctgtggg atggtaaacg ttatcgtcag ctggtcgctc gtacaggtga
      901 aaatggtgtg gaagccgaca ttccgtatta tgtgaatgag gacgatgata ttgtggacaa
      961 accggacgaa gatgatgatt ggatcgaagt gaaaggtact agcagcagca ttgcgagcag
     1021 cagcccgagc agcgtggcgg gatctatggc acaggttctg cgtggcaccg ttaccgattt
     1081 tccgggtttt gatgaacgtg cagatgcaga aaccctgcgt aaagcaatga aaggtctggg
     1141 caccgatgaa gaaagcattc tgaccctgct gaccagccgt agcaatgcac agcgtcaaga
     1201 aattagcgca gcatttaaaa ccctgtttgg tcgtgatctg ctggatgatc tgaaaagcga
     1261 actgaccggc aaatttgaaa aactgattgt tgcactgatg aaaccgagcc gtctgtatga
     1321 tgcatatgaa ctgaaacatg cactgaaagg tgcaggcacc aatgaaaaag tgctgaccga
     1381 aattattgca agccgtacac cggaagaact gcgtgcaatt aaacaggttt atgaagagga
     1441 atatggtagc agcctggaag atgatgttgt tggtgatacc agcggttatt atcagcgtat
     1501 gctggttgtt ctgctgcaag caaatcgtga tccggatgca ggtattgatg aagcacaggt
     1561 tgaacaggat gcacaggcac tgtttcaggc aggcgaactg aaatggggta cagatgaaga
     1621 gaaattcatt accatttttg gcacccgtag cgttagccat ctgcgcaaag tttttgataa
     1681 atacatgacc attagcggct tccagattga agaaaccatt gatcgtgaaa ccagtggtaa
     1741 tctggaacaa ctgctgctgg cagttgttaa aagcattcgt agcattccgg catatctggc
     1801 cgaaaccctg tattatgcca tgaaaggcgc aggtacggat gatcacaccc tgattcgtgt
     1861 tatggttagc cgtagcgaaa ttgacctgtt caatatccgt aaagagttcc gcaaaaattt
     1921 cgcaaccagc ctgtatagca tgattaaagg tgatacctca ggcgactaca aaaaagccct
     1981 gctgctgctg tgcggtgaag atgattaata atactagagt cacactggct caccttcggg
     2041 tgggcctttc tgcgtttata tactagagtt tatggctagc tcagtcctag gtacaatgct
     2101 agctactaga gaaagaggag aaatactaga tggcttcctc cgaagacgtt atcaaagagt
     2161 tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg tcacgagttc gaaatcgaag
     2221 gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc taaactgaaa gttaccaaag
     2281 gtggtccgct gccgttcgct tgggacatcc tgtccccgca gttccagtac ggttccaaag
     2341 cttacgttaa acacccggct gacatcccgg actacctgaa actgtccttc ccggaaggtt
     2401 tcaaatggga acgtgttatg aacttcgaag acggtggtgt tgttaccgtt acccaggact
     2461 cctccctgca agacggtgag ttcatctaca aagttaaact gcgtggtacc aacttcccgt
     2521 ccgacggtcc ggttatgcag aaaaaaacca tgggttggga agcttccacc gaacgtatgt
     2581 acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg tctgaaactg aaagacggtg
     2641 gtcactacga cgctgaagtt aaaaccacct acatggctaa aaaaccggtt cagctgccgg
     2701 gtgcttacaa aaccgacatc aaactggaca tcacctccca caacgaagac tacaccatcg
     2761 ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg tgcttaataa
//