LOCUS       BBa_K185001             1791 bp    DNA     linear       23-Apr-2024
DEFINITION  Arabinose-induced RelE Generator
ACCESSION   BBa_K185001
VERSION     BBa_K185001.1  
FEATURES             Location/Qualifiers
     promoter        1..1342
                     /label=BBa_K185052
     misc_feature    1349..1351
                     /label=start
     CDS             1352..1633
                     /label=cds
     misc_feature    1654..1654
                     /label=stop
     misc_feature    1653..1654
                     /label=mutation
     misc_feature    1634..1651
                     /label=His tag
     CDS             1349..1654
                     /label=BBa_K185047
     stem_loop       1674..1717
                     /label=stem_loop
     misc_feature    1663..1742
                     /label=BBa_B0010
     terminator      1663..1742
                     /label=BBa_B0010
     stem_loop       1758..1777
                     /label=T7 TE
     misc_feature    1784..1784
                     /label=stop
     polyA_site      1778..1791
                     /label=polya
     misc_feature    1751..1791
                     /label=BBa_B0012
     terminator      1751..1791
                     /label=BBa_B0012
ORIGIN
        1 cgatgcataa tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc tatgctactc
       61 cgtcaagccg tcaattgtct gattcgttac caattatgac aacttgacgg ctacatcatt
      121 cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc attttttaaa
      181 tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg tggcgatagg
      241 catccgggtg gtgctcaaaa gcagcttcgc ctggctgata cgttggtcct cgcgccagct
      301 taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg gcgacaagca
      361 aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat cgctgatgta
      421 ctgacaagcc tcgcgtaccc gattatccat cggtggatgg agcgactcgt taatcgcttc
      481 catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg aatagcgccc
      541 ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg gctggtgcgc
      601 ttcatccggg cgaaagaacc ccgtattggc aaatattgac ggccagttaa gccattcatg
      661 ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag cctccggatg
      721 acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg gtcggcaaac
      781 aaattctcgt ccctgatttt tcaccacccc ctgaccgcga atggtgagat tgagaatata
      841 acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg cctcaatcgg
      901 cgttaaaccc gccaccagat gggcattaaa cgagtatccc ggcagcaggg gatcattttg
      961 cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt gtccatattg
     1021 catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaacca aaccggtaac
     1081 cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa aaacgcgtaa
     1141 caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg gcgtcacact
     1201 ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac gctttttatc
     1261 gcaactctct actgtttctc catacccgtt tttttgggct agaaataatt ttgtttaact
     1321 ttaagaagga gatatacata tgtactagat ggcgtatttt ctggattttg acgagcgggc
     1381 actaaaggaa tggcgaaagc tgggctcgac ggtacgtgaa cagttgaaaa agaagctggt
     1441 tgaagtactt gagtcacccc ggattgaagc aaacaagctc cgtggtatgc ctgattgtta
     1501 caagattaag ctccggtctt caggctatcg ccttgtatac caggttatag acgagaaagt
     1561 tgtcgttttc gtgatttctg ttgggaaaag agaacgctcg gaagtatata gcgaggcggt
     1621 caaacgcatt ctccatcatc atcatcatca ctaatactag agccaggcat caaataaaac
     1681 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     1741 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
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