LOCUS       BBa_K185003             2827 bp    DNA     linear       03-May-2024
DEFINITION  RelE toxin+Lon protease
ACCESSION   BBa_K185003
VERSION     BBa_K185003.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=start
     CDS             4..285
                     /label=cds
     misc_feature    306..306
                     /label=stop
     misc_feature    305..306
                     /label=mutation
     misc_feature    286..303
                     /label=His tag
     CDS             1..306
                     /label=BBa_K185047
     RBS             315..329
                     /label=RBS-1\Strong
     RBS             322..326
                     /label=RBS
     misc_feature    315..329
                     /label=BBa_B0030
     RBS             315..329
                     /label=BBa_B0030
     misc_feature    336..338
                     /label=start
     misc_feature    2688..2690
                     /label=2353
     CDS             336..2690
                     /label=cds
     CDS             336..2690
                     /label=BBa_K185049
     stem_loop       2710..2753
                     /label=stem_loop
     misc_feature    2699..2778
                     /label=BBa_B0010
     terminator      2699..2778
                     /label=BBa_B0010
     stem_loop       2794..2813
                     /label=T7 TE
     misc_feature    2820..2820
                     /label=stop
     polyA_site      2814..2827
                     /label=polya
     misc_feature    2787..2827
                     /label=BBa_B0012
     terminator      2787..2827
                     /label=BBa_B0012
ORIGIN
        1 atggcgtatt ttctggattt tgacgagcgg gcactaaagg aatggcgaaa gctgggctcg
       61 acggtacgtg aacagttgaa aaagaagctg gttgaagtac ttgagtcacc ccggattgaa
      121 gcaaacaagc tccgtggtat gcctgattgt tacaagatta agctccggtc ttcaggctat
      181 cgccttgtat accaggttat agacgagaaa gttgtcgttt tcgtgatttc tgttgggaaa
      241 agagaacgct cggaagtata tagcgaggcg gtcaaacgca ttctccatca tcatcatcat
      301 cactaatact agagattaaa gaggagaaat actagatgaa tcctgagcgt tctgaacgca
      361 ttgaaatccc cgtattgccg ctgcgcgatg tggtggttta tccgcacatg gtcatcccct
      421 tatttgtcgg gcgggaaaaa tctatccgtt gtctggaagc ggcgatggac catgataaaa
      481 aaattatgct ggtcgcgcag aaagaagctt caacggatga gccgggtgta aacgatcttt
      541 tcaccgtcgg gaccgtggcc tctatattgc agatgctgaa actgcctgac ggcaccgtca
      601 aagtgctggt cgaggggtta cagcgcgcgc gtatttctgc gctctctgac aatggcgaac
      661 acttttctgc gaaggcggag tatctggagt cgccgaccat tgatgagcgg gaacaggaag
      721 tgctggtgcg tactgcaatc agccagttcg aaggctacat caagctgaac aaaaaaatcc
      781 caccagaagt gctgacgtcg ctgaatagca tcgacgatcc ggcgcgtctg gcggatacca
      841 ttgctgcaca tatgccgctg aaactggctg acaaacagtc tgttctggag atgtccgacg
      901 ttaacgaacg tctggaatat ctgatggcaa tgatggaatc ggaaatcgat ctgctgcagg
      961 ttgagaaacg cattcgcaac cgcgttaaaa agcagatgga gaaatcccag cgtgagtact
     1021 atctgaacga gcaaatgaaa gctattcaga aagaactcgg tgaaatggac gacgcgccgg
     1081 acgaaaacga agccctgaag cgcaaaatcg acgcggcgaa gatgccgaaa gaggcaaaag
     1141 agaaagcgga agcagagttg cagaagctga aaatgatgtc tccgatgtcg gcagaagcga
     1201 ccgtagtgcg tggttatatc gactggatgg tacaggtgcc gtggaatgcg cgtagcaagg
     1261 tcaaaaaaga cctgcgtcag gcgcaggaaa tccttgatac cgaccattat ggtctggagc
     1321 gcgtgaaaga tcgaatcctt gagtatcttg cggttcaaag ccgtgtcaac aaaatcaagg
     1381 gaccgatcct ctgcctggta gggccgccgg gggtaggtaa aacctctctt ggtcagtcca
     1441 ttgccaaagc caccgggcgt aaatatgtcc gtatggcgct gggcggcgtg cgtgatgaag
     1501 cggaaatccg tggtcaccgc cgtacttaca tcggttctat gccgggtaaa ctgatccaga
     1561 aaatggcgaa agtgggcgtg aaaaacccgc tgttcctgct cgatgagatc gacaaaatgt
     1621 cttctgacat gcgtggcgat ccggcctctg cactgcttga agtgctggat ccagagcaga
     1681 acgtagcgtt cagcgaccac tacctggaag tggattacga tctcagcgac gtgatgtttg
     1741 tcgcgacgtc gaactccatg aacattccgg caccgctgct ggatcgtatg gaagtgattc
     1801 gcctctccgg ttataccgaa gatgaaaaac tgaacatcgc caaacgtcac ctgctgccga
     1861 agcagattga acgtaatgca ctgaaaaaag gtgagctgac cgtcgacgat agcgccatta
     1921 tcggcattat tcgttactac acccgtgagg cgggcgtgcg tggtctggag cgtgaaatct
     1981 ccaaactgtg tcgcaaagcg gttaagcagt tactgctcga taagtcatta aaacatatcg
     2041 aaattaacgg cgataacctg catgactatc tcggtgttca gcgtttcgac tatggtcgcg
     2101 cggataacga aaaccgtgtc ggtcaggtaa ccggtctggc gtggacggaa gtgggcggtg
     2161 acttgctgac cattgaaacc gcatgtgttc cgggtaaagg caaactgacc tataccggtt
     2221 cgctcggcga agtgatgcag gagtccattc aggcggcgtt aacggtggtt cgtgcgcgtg
     2281 cggaaaaact ggggatcaac cctgattttt acgaaaaacg tgacatccac gtccacgtac
     2341 cggaaggtgc gacgccgaaa gatggtccga gtgccggtat tgctatgtgc accgcgctgg
     2401 tttcttgcct gaccggtaac ccggttcgtg ccgatgtggc aatgaccggt gagatcactc
     2461 tgcgtggtca ggtactgccg atcggtggtt tgaaagaaaa actcctggca gcgcatcgcg
     2521 gcgggattaa aacagtgcta attccgttcg aaaataaacg cgatctggaa gagattcctg
     2581 acaacgtaat tgccgatctg gacattcatc ctgtgaagcg cattgaggaa gttctgactc
     2641 tggcgctgca aaatgaaccg tctggtatgc aggttgtgac tgcaaaatag tactagagcc
     2701 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     2761 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
     2821 gtttata
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