LOCUS       BBa_K185018             1454 bp    DNA     linear       23-May-2024
DEFINITION  Promoter118+QPI+RBS30+RelB+Double Terminator
ACCESSION   BBa_K185018
VERSION     BBa_K185018.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23118
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             65..772
                     /label=cI lambda
     misc_feature    773..805
                     /label=LVA
     misc_feature    812..836
                     /label=Help:Barcodes
     CDS             62..811
                     /label=BBa_C0051
     stem_loop       856..899
                     /label=stem_loop
     misc_feature    845..924
                     /label=BBa_B0010
     terminator      845..924
                     /label=BBa_B0010
     stem_loop       940..959
                     /label=T7 TE
     misc_feature    966..966
                     /label=stop
     polyA_site      960..973
                     /label=polya
     misc_feature    933..973
                     /label=BBa_B0012
     terminator      933..973
                     /label=BBa_B0012
     promoter        1019..1024
                     /label=-10
     promoter        996..1001
                     /label=-35
     misc_feature    1006..1022
                     /label=OR1
     misc_feature    982..998
                     /label=OR2
     misc_feature    982..1030
                     /label=BBa_R0051
     promoter        982..1030
                     /label=BBa_R0051
     misc_feature    44..1030
                     /label=BBa_Q04510
     RBS             1039..1053
                     /label=RBS-1\Strong
     RBS             1046..1050
                     /label=RBS
     misc_feature    1039..1053
                     /label=BBa_B0030
     RBS             1039..1053
                     /label=BBa_B0030
     misc_feature    1060..1062
                     /label=start
     CDS             1060..1299
                     /label=cds
     misc_feature    1315..1317
                     /label=stop
     misc_feature    1300..1314
                     /label=His tag
     CDS             1060..1317
                     /label=BBa_K185048
     stem_loop       1337..1380
                     /label=stem_loop
     misc_feature    1326..1405
                     /label=BBa_B0010
     terminator      1326..1405
                     /label=BBa_B0010
     stem_loop       1421..1440
                     /label=T7 TE
     misc_feature    1447..1447
                     /label=stop
     polyA_site      1441..1454
                     /label=polya
     misc_feature    1414..1454
                     /label=BBa_B0012
     terminator      1414..1454
                     /label=BBa_B0012
     terminator      1326..1454
                     /label=BBa_B0015
ORIGIN
        1 ttgacggcta gctcagtcct aggtattgtg ctagctacta gagaaagagg agaaatacta
       61 gatgagcaca aaaaagaaac cattaacaca agagcagctt gaggacgcac gtcgccttaa
      121 agcaatttat gaaaaaaaga aaaatgaact tggcttatcc caggaatctg tcgcagacaa
      181 gatggggatg gggcagtcag gcgttggtgc tttatttaat ggcatcaatg cattaaatgc
      241 ttataacgcc gcattgcttg caaaaattct caaagttagc gttgaagaat ttagcccttc
      301 aatcgccaga gaaatctacg agatgtatga agcggttagt atgcagccgt cacttagaag
      361 tgagtatgag taccctgttt tttctcatgt tcaggcaggg atgttctcac ctgagcttag
      421 aacctttacc aaaggtgatg cggagagatg ggtaagcaca accaaaaaag ccagtgattc
      481 tgcattctgg cttgaggttg aaggtaattc catgaccgca ccaacaggct ccaagccaag
      541 ctttcctgac ggaatgttaa ttctcgttga ccctgagcag gctgttgagc caggtgattt
      601 ctgcatagcc agacttgggg gtgatgagtt taccttcaag aaactgatca gggatagcgg
      661 tcaggtgttt ttacaaccac taaacccaca gtacccaatg atcccatgca atgagagttg
      721 ttccgttgtg gggaaagtta tcgctagtca gtggcctgaa gagacgtttg gcgctgcaaa
      781 cgacgaaaac tacgctttag tagcttaata acgctgatag tgctagtgta gatcgctact
      841 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
      901 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
      961 ttctgcgttt atatactaga gtaacaccgt gcgtgttgac tattttacct ctggcggtga
     1021 taatggttgc tactagagat taaagaggag aaatactaga tgggtagcat taacctgcgt
     1081 attgacgatg aacttaaagc gcgttcttac gccgcgcttg aaaaaatggg tgtaactcct
     1141 tctgaagcgc ttcgtctcat gctcgagtat atcgctgaca atgaacgctt gccgttcaaa
     1201 cagacactcc tgagtgatga agatgctgaa cttgtggaga tagtgaaaga acggcttcgt
     1261 aatcctaagc cagtacgtgt gacgctggat gaactccatc atcatcatca tcactgatac
     1321 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
     1381 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
     1441 tttctgcgtt tata
//