LOCUS       BBa_K185030              357 bp    DNA     linear       27-May-2024
DEFINITION  dter-plac-rbs34 intermediate
ACCESSION   BBa_K185030
VERSION     BBa_K185030.1  
FEATURES             Location/Qualifiers
     stem_loop       12..55
                     /label=stem_loop
     misc_feature    1..80
                     /label=BBa_B0010
     terminator      1..80
                     /label=BBa_B0010
     stem_loop       96..115
                     /label=T7 TE
     misc_feature    122..122
                     /label=stop
     polyA_site      116..129
                     /label=polya
     misc_feature    89..129
                     /label=BBa_B0012
     terminator      89..129
                     /label=BBa_B0012
     CDS             138..225
                     /label=end of LacI coding region (inactive)
     misc_feature    138..337
                     /label=BBa_R0010
     misc_feature    226..263
                     /label=CAP binding site
     promoter        274..279
                     /label=-35
     promoter        298..303
                     /label=-10
     misc_feature    303..337
                     /label=LacI binding site
     misc_feature    310..310
                     /label=start
     promoter        138..337
                     /label=BBa_R0010
     misc_feature    350..353
                     /label=conserved
     RBS             346..357
                     /label=BBa_B0034
ORIGIN
        1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
       61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      121 gcgtttatat actagagcaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
      181 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
      241 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
      301 gttgtgtgga attgtgagcg gataacaatt tcacacatac tagagaaaga ggagaaa
//