BBa_K1855004 1 BBa_K1855004 INP-Zif268 2015-09-16T11:00:00Z 2015-09-17T03:12:50Z Lpp-OmpA is originally from Pseudomonas syringae; Zif268 is from mice. A fusion protein of a transmembrane domain (INP) and a zinc-finger domain (Zif268 1AAY fragment),under pL_lac promotion and containing a C-terminal FLAG tag. It is used to exhibit a zinc-finger dsDNA binding domain complimentary to CCGCCCNCGC on the cell surface of E.coli. false false _2282_ 27125 27125 9 false We had to optimise the codon for E.coli K12, and then alter the codon slightly to remove unwanted cut sites. The INP domain is preceeded by an NdeI cut site, and followed by an AgeI cut site. The Zif268 fragment domain is preceeded by an NheI cut site and followed by a ClaI cut site. false Iain Galpin annotation2466886 1 INP range2466886 1 84 1007 annotation2466890 1 3x FLAG range2466890 1 1353 1424 annotation2466889 1 Linker range2466889 1 1326 1349 annotation2466888 1 Zif268 range2466888 1 1053 1319 annotation2466885 1 B0034 Strong RBS range2466885 1 60 80 annotation2466887 1 Linker range2466887 1 1011 1046 annotation2466884 1 pL_lac range2466884 1 1 56 annotation2466891 1 BBa_K864600 T0 Terminator range2466891 1 1431 1482 BBa_K1855004_sequence 1 ataaatgtgagcggataacattgacattgtgagcggataacaagatactgagcacattaattaaagaggagaaaggtacccatatgaccctggataaagcactggtgctgcgcacctgcgctaacaacatggcggatcactgcggcctgatctggccagccagcggcaccgtagaatctcgttattggcaatccactcgtcgccacgagaacggcctggttggtctgctgtggggtgcgggtacgtctgcattcctgagcgttcatgctgacgcacgttggatcgtgtgcgaagtagctgttgcggacatcatctccctggaggaaccaggtatggttaaattcccgcgtgcggaagttgtgcacgtcggtgatcgcattagcgcgagccacttcatttctgcgcgccaggctgacccggcatctacctctacctctacctccacttctaccctgaccccgatgccgactgctatcccgacgccgatgccagcagtggcatctgtaaccctgccggttgccgaacaggctcgccatgaagtcttcgacgttgcctctgtgagcgctgcggcggcgccggttaacaccctgcctgttaccactccgcagaacctgcaaactgcgacgtacggttccaccctgtccggcgataaccattcccgtctgattgctggttacggttctaacgaaacggccggtaaccacagcgatctgatcggtggtcacgactgtaccctgatggctggtgatcagtctcgtctgaccgctggtaaaaattccgtactgaccgcaggtgcgcgctctaaactgattggtagcgaaggttctaccctgtccgcaggcgaagatagcaccctgatttttcgcctgtgggatggtaaacgttaccgtcagctggttgcgcgtaccggcgaaaacggtgttgaagccgacatcccgtactacgtcaacgaagacgacgacatcgtcgacaagccggatgaagacgacgattggatcgaggttaagaccggtggctctggcggtggctccggtggcggctccggcgctagcgaacgtccttatgcttgtccagtggagtcttgcgatcgccgtttctcccgtagcgacgaactgacccgtcacatccgtatccacaccggccagaagccgtttcaatgccgtatctgtatgcgtaacttttcccgcagcgatcacctgactacccacatccgtacccatactggtgaaaaaccgttcgcgtgcgatatctgtggtcgcaaattcgcacgcagcgacgaacgcaaacgtcatactaaaattcacctgcgtcagaaagatatcgatggtggcggttccggcggcggttcccatgattataaagatgacgatgacaaagactacaaggacgatgacgacaaggactataaggacgacgacgataagtgataattgttcagaacgctcggtcttgcacaccgggcgttttttctttgtgagtcca igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z