LOCUS       BBa_K1859026            1464 bp    DNA     linear       17-May-2024
DEFINITION  mRNA circularize efficiently generator (outside complementarity)
ACCESSION   BBa_K1859026
VERSION     BBa_K1859026.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    209..414
                     /label=BBa_K1859015
     misc_feature    427..430
                     /label=conserved
     RBS             423..434
                     /label=BBa_B0034
     CDS             443..1145
                     /label=BBa_K1332002
     misc_feature    1154..1327
                     /label=BBa_K1859016
     stem_loop       1347..1390
                     /label=stem_loop
     misc_feature    1336..1415
                     /label=BBa_B0010
     terminator      1336..1415
                     /label=BBa_B0010
     stem_loop       1431..1450
                     /label=T7 TE
     misc_feature    1457..1457
                     /label=stop
     polyA_site      1451..1464
                     /label=polya
     misc_feature    1424..1464
                     /label=BBa_B0012
     terminator      1424..1464
                     /label=BBa_B0012
     terminator      1336..1464
                     /label=BBa_B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa tctgataaat ggaattggtt ctacataaat
      241 gcctaacgac tatccctttg gggagtaggg tcaagtgact cgaaacgata gacaacttgc
      301 tttaacaagt tggagatata gtctgctctg catggtgaca tgcagctgga tataattccg
      361 gggtaagatt aacgacctta tctgaacata atgctaccgt ttaatattgc gtcatactag
      421 agaaagagga gaaatactag agagatgcat catcatcatc atcatcatca tatggcttcc
      481 tccgaagacg ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg ttccgttaac
      541 ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg tacccagacc
      601 gctaaactga aagttaccaa aggtggtccg ctgccgttcg cttgggacat cctgtccccg
      661 cagttccagt acggttccaa agcttacgtt aaacacccgg ctgacatccc ggactacctg
      721 aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga agacggtggt
      781 gttgttaccg ttacccagga ctcctccctg caagacggtg agttcatcta caaagttaaa
      841 ctgcgtggta ccaacttccc gtccgacggt ccggttatgc agaaaaaaac catgggttgg
      901 gaagcttcca ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga aatcaaaatg
      961 cgtctgaaac tgaaagacgg tggtcactac gacgctgaag ttaaaaccac ctacatggct
     1021 aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga catcacctcc
     1081 cacaacgaag actacaccat cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc
     1141 ggtgctacta gagcagagat gttttcttgg gttaattgag gcctgagtat aaggtgactt
     1201 atacttgtaa tctatctaaa cggggaacct ctctagtaga caatcccgtg ctaaattgta
     1261 ggactgccct ttaataaata cttctatatt taaagaggta tttatgaaaa gcggaattta
     1321 tcagatttac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     1381 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
     1441 cgggtgggcc tttctgcgtt tata
//