LOCUS       BBa_K1859027            1420 bp    DNA     linear       19-May-2024
DEFINITION  Histidine tag and RFP linker [GGSGGS] semi-permanent generator
ACCESSION   BBa_K1859027
VERSION     BBa_K1859027.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    209..414
                     /label=BBa_K1859001
     misc_feature    427..430
                     /label=conserved
     RBS             423..434
                     /label=BBa_B0034
     CDS             443..1145
                     /label=BBa_K1332002
     misc_feature    1154..1283
                     /label=BBa_K1859004
     stem_loop       1303..1346
                     /label=stem_loop
     misc_feature    1292..1371
                     /label=BBa_B0010
     terminator      1292..1371
                     /label=BBa_B0010
     stem_loop       1387..1406
                     /label=T7 TE
     misc_feature    1413..1413
                     /label=stop
     polyA_site      1407..1420
                     /label=polya
     misc_feature    1380..1420
                     /label=BBa_B0012
     terminator      1380..1420
                     /label=BBa_B0012
     terminator      1292..1420
                     /label=BBa_B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagaggg ttctacataa atgcctaacg actatccctt
      241 tggggagtag ggtcaagtga ctcgaaacga tagacaactt gctttaacaa gttggagata
      301 tagtctgctc tgcatggtga catgcagctg gatataattc cggggtaaga ttaacgacct
      361 tatctgaaca taatgctacc gtttaatatt gcgtcagggt ggtagtggtg gtagtactag
      421 agaaagagga gaaatactag agagatgcat catcatcatc atcatcatca tatggcttcc
      481 tccgaagacg ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg ttccgttaac
      541 ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg tacccagacc
      601 gctaaactga aagttaccaa aggtggtccg ctgccgttcg cttgggacat cctgtccccg
      661 cagttccagt acggttccaa agcttacgtt aaacacccgg ctgacatccc ggactacctg
      721 aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga agacggtggt
      781 gttgttaccg ttacccagga ctcctccctg caagacggtg agttcatcta caaagttaaa
      841 ctgcgtggta ccaacttccc gtccgacggt ccggttatgc agaaaaaaac catgggttgg
      901 gaagcttcca ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga aatcaaaatg
      961 cgtctgaaac tgaaagacgg tggtcactac gacgctgaag ttaaaaccac ctacatggct
     1021 aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga catcacctcc
     1081 cacaacgaag actacaccat cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc
     1141 ggtgctacta gaggtggtag tggtggtagt tcagagatgt tttcttgggt taattgaggc
     1201 ctgagtataa ggtgacttat acttgtaatc tatctaaacg gggaacctct ctagtagaca
     1261 atcccgtgct aaattgtagg acttactaga gccaggcatc aaataaaacg aaaggctcag
     1321 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
     1381 cacactggct caccttcggg tgggcctttc tgcgtttata
//