LOCUS       BBa_K1859029            1420 bp    DNA     linear       17-May-2024
DEFINITION  Histidine tag and RFP linker [HHHHHH] semi-permanent generator
ACCESSION   BBa_K1859029
VERSION     BBa_K1859029.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    209..411
                     /label=BBa_K1859003
     misc_feature    424..427
                     /label=conserved
     RBS             420..431
                     /label=BBa_B0034
     CDS             440..1142
                     /label=BBa_K1332002
     misc_feature    1151..1283
                     /label=BBa_K1859006
     stem_loop       1303..1346
                     /label=stem_loop
     misc_feature    1292..1371
                     /label=BBa_B0010
     terminator      1292..1371
                     /label=BBa_B0010
     stem_loop       1387..1406
                     /label=T7 TE
     misc_feature    1413..1413
                     /label=stop
     polyA_site      1407..1420
                     /label=polya
     misc_feature    1380..1420
                     /label=BBa_B0012
     terminator      1380..1420
                     /label=BBa_B0012
     terminator      1292..1420
                     /label=BBa_B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagaggg ttctacataa atgcctaacg actatccctt
      241 tggggagtag ggtcaagtga ctcgaaacga tagacaactt gctttaacaa gttggagata
      301 tagtctgctc tgcatggtga catgcagctg gatataattc cggggtaaga ttaacgacct
      361 tatctgaaca taatgctacc gtttaatatt gcgtcaccac caccaccacc atactagaga
      421 aagaggagaa atactagaga gatgcatcat catcatcatc atcatcatat ggcttcctcc
      481 gaagacgtta tcaaagagtt catgcgtttc aaagttcgta tggaaggttc cgttaacggt
      541 cacgagttcg aaatcgaagg tgaaggtgaa ggtcgtccgt acgaaggtac ccagaccgct
      601 aaactgaaag ttaccaaagg tggtccgctg ccgttcgctt gggacatcct gtccccgcag
      661 ttccagtacg gttccaaagc ttacgttaaa cacccggctg acatcccgga ctacctgaaa
      721 ctgtccttcc cggaaggttt caaatgggaa cgtgttatga acttcgaaga cggtggtgtt
      781 gttaccgtta cccaggactc ctccctgcaa gacggtgagt tcatctacaa agttaaactg
      841 cgtggtacca acttcccgtc cgacggtccg gttatgcaga aaaaaaccat gggttgggaa
      901 gcttccaccg aacgtatgta cccggaagac ggtgctctga aaggtgaaat caaaatgcgt
      961 ctgaaactga aagacggtgg tcactacgac gctgaagtta aaaccaccta catggctaaa
     1021 aaaccggttc agctgccggg tgcttacaaa accgacatca aactggacat cacctcccac
     1081 aacgaagact acaccatcgt tgaacagtac gaacgtgctg aaggtcgtca ctccaccggt
     1141 gctactagag gccaccacca ccaccaccac acagagatgt tttcttgggt taattgaggc
     1201 ctgagtataa ggtgacttat acttgtaatc tatctaaacg gggaacctct ctagtagaca
     1261 atcccgtgct aaattgtagg acttactaga gccaggcatc aaataaaacg aaaggctcag
     1321 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
     1381 cacactggct caccttcggg tgggcctttc tgcgtttata
//