LOCUS       BBa_K1861030            1349 bp    DNA     linear       27-May-2024
DEFINITION  eYFP gene for expression in yeast
ACCESSION   BBa_K1861030
VERSION     BBa_K1861030.1  
FEATURES             Location/Qualifiers
     misc_feature    1..51
                     /label=Homologous recombination site
     misc_feature    52..64
                     /label=TALE3 binding site
     misc_feature    65..70
                     /label=SacI restriction site
     misc_feature    1..71
                     /label=BBa_K1861301
     promoter        72..275
                     /label=CMV promoter
     misc_feature    137..149
                     /label=TALE1 binding site
     misc_feature    201..213
                     /label=TALE2 binding site
     promoter        72..275
                     /label=BBa_K1861303
     misc_feature    276..281
                     /label=BamHI restriction site
     RBS             282..296
                     /label=rbs
     misc_feature    294..296
                     /label=start
     RBS             276..296
                     /label=BBa_K792001
     misc_feature    297..299
                     /label=ATG start codon
     misc_feature    300..305
                     /label=XhoI restriction site
     misc_feature    1056..1061
                     /label=SalI restriction site
     misc_feature    1053..1055
                     /label=TAA stop codon
     misc_feature    297..1061
                     /label=BBa_K1861302
     misc_feature    1062..1286
                     /label=ADH1 terminator
     terminator      1062..1286
                     /label=BBa_J63002
     misc_feature    1287..1292
                     /label=BclI restriction site
     misc_feature    1287..1335
                     /label=Homologous recombination site
     misc_feature    1337..1349
                     /label=TALE3 binding site
     misc_feature    1287..1349
                     /label=BBa_K1861304
ORIGIN
        1 gttggtgagc gctaggagtc actgccaggt atcgtttgaa cacggcatta gttccgactc
       61 gctgtgagct cgtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      121 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      181 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      241 ggcgtgtacg gtgggaggtc tatataagca gagctggatc cacgattaaa agaatgatgc
      301 tcgagatggc aactagcggc atggttagta aaggagaaga acttttcact ggagttgtcc
      361 caattttagt tgaactagat ggcgacgtga acggtcataa gttcagtgtc tccggcgaag
      421 gtgagggtga tgcaacgtac ggtaagttaa ctttgaagtt aatatgtaca accggcaagc
      481 tgcctgttcc ctggcctacc ctggtgacaa cgttaggtta tgggttgatg tgctttgcta
      541 gatacccaga tcacatgaaa aggcatgact tctttaaatc tgcaatgcca gaaggttacg
      601 tccaagaacg tactattttc tttaaagatg acggtaatta taaaactagg gctgaagtta
      661 aattcgaagg tgacacactt gtaaatcgaa tagagttaaa ggggattgat ttcaaagagg
      721 atggtaatat tctaggccat aaacttgaat ataactataa ttcacacaac gtttacatta
      781 ccgccgacaa gcagaagaat ggaatcaaag ccaattttaa gattagacac aatattgagg
      841 atggtggagt acagcttgct gatcattacc aacaaaatac cccgatcggt gatggaccag
      901 ttttgctacc cgataaccat tatctgtcct atcaaagcaa attgtcaaaa gatcctaacg
      961 aaaaaagaga ccacatggta ctcttggaat ttgtaacagc tgctgggatt acacatggca
     1021 tggatgaact atacaaaggt tctggtaccg cataagtcga ctaataagcg aatttcttat
     1081 gatttatgat ttttattatt aaataagtta taaaaaaaat aagtgtatac aaattttaaa
     1141 gtgactctta ggttttaaaa cgaaaattct tattcttgag taactctttc ctgtaggtca
     1201 ggttgctttc tcaggtatag catgaggtcg ctcttattga ccacacctct accggcatgc
     1261 cgagcaaatg cctgcaaatc gctccctgat catcaccgta gtgagagtgc gttcaaggct
     1321 cttgcggttg ccatattccg actcgctgt
//