LOCUS       BBa_K1861060            2652 bp    DNA     linear       02-May-2024
DEFINITION  Fusion protein of TALE2 and a SpyTag in an expression cassette
ACCESSION   BBa_K1861060
VERSION     BBa_K1861060.1  
FEATURES             Location/Qualifiers
     misc_feature    133..133
                     /label=T->C mutation to avoid SpeI restriction site
     promoter        1..238
                     /label=rpd3 promoter
     promoter        1..238
                     /label=BBa_K1861101
     misc_feature    239..241
                     /label=ATG start codon
     misc_feature    242..259
                     /label=6x His-Tag
     misc_feature    260..265
                     /label=BamHI restriction site
     misc_feature    239..265
                     /label=BBa_K1861102
     misc_feature    266..268
                     /label=start codon ATG
     CDS             266..724
                     /label=Front constant domain
     CDS             725..1960
                     /label=Variable domain
     CDS             1961..2476
                     /label=Back constant domain
     CDS             266..2475
                     /label=BBa_K1861601
     misc_feature    2476..2514
                     /label=SpyTag
     misc_feature    2494..2496
                     /label=Reactive Asp for isopeptide forming reaction
     CDS             2476..2514
                     /label=BBa_K1159201
     misc_feature    2515..2520
                     /label=SacI restriction site
     misc_feature    2521..2523
                     /label=TGA stop codon
     misc_feature    2515..2523
                     /label=BBa_K1861104
     terminator      2524..2652
                     /label=BBa_K392003
ORIGIN
        1 gtaatatcaa ctcagagcgt ataggtaaat ttgtaaatat atgtcccata ttttgccttg
       61 aaatttatct ttttatttac ttaccctccc cggctcatag tatctatcga aagggaaaaa
      121 cagaaaagat accagtagtt gattatacat aataaaaatt tgaagtaata accataaagg
      181 ttcataaaac aattgcgcca tacaaaacat tcgtggctac aactcgatat ccgtgcagat
      241 gcatcaccac catcatcacg gatccatggc ccccaagaag aagaggaagg tgggcattca
      301 ccgcggggta cctatggtgg acttgaggac actcggttat tcgcaacagc aacaggagaa
      361 aatcaagcct aaggtcagga gcaccgtcgc gcaacaccac gaggcgcttg tggggcatgg
      421 cttcactcat gcgcatattg tcgcgctttc acagcaccct gcggcgcttg ggacggtggc
      481 tgtcaaatac caagatatga ttgcggccct gcccgaagcc acgcacgagg caattgtagg
      541 ggtcggtaaa cagtggtcgg gagcgcgagc acttgaggcg ctgctgactg tggcgggtga
      601 gcttaggggg cctccgctcc agctcgacac cgggcagctg ctgaagatcg cgaagagagg
      661 gggagtaaca gcggtagagg cagtgcacgc ctggcgcaat gcgctcaccg gtgcccccct
      721 gaatctaacc cctgaccagg tagttgccat cgcttccaac cacggcggaa aacaggccct
      781 agaaaccgtt cagcgtttgt tgccagtctt atgccaagac catggattga ctccagacca
      841 agtcgtcgct atcgcctcaa acggtggagg gaagcaagca ttggaaacag tccaaagact
      901 gctacctgtc ctgtgtcagg accacgggtt aactccggac caggttgtgg ccatagcctc
      961 tcatgatggt ggtaagcagg cacttgaaac ggtacagaga ctactacccg tgctttgtca
     1021 ggaccacgga cttacccccg atcaagtagt agcgattgcg agtaaccatg gcggtaaaca
     1081 agcccttgaa actgtgcaaa gattgctgcc tgtattgtgc caggatcacg ggcttacacc
     1141 ggatcaggtc gtagcaatag catctaatgg aggtggaaag caagctttgg agactgttca
     1201 acgtttattg cctgtcttgt gtcaagatca tgggctgaca cccgaccaag tggtagccat
     1261 tgcctctaac attgggggca aacaggcttt agagacagtg cagcgtcttt tgcccgtttt
     1321 gtgcaggatc acggcttgac tccggatcag gtcgtggcga tagcgagcaa tataggcggg
     1381 aaacaggcgc tagaaactgt ccaaagatta ttaccggttt tatgtcagga ccatgggcta
     1441 acccctgatc aagtcgtcgc catagcgtcc cacgacggag gtaaacaagc gttggagact
     1501 gtgcagcgtc tattgcccgt gctttgtcaa gaccacggac ttacgccaga ccaggttgtg
     1561 gctattgcaa gtaacatagg tggcaaacag gctttggaaa cggtacaaag actactgcca
     1621 gtcttatgcc aagatcatgg attgacacct gaccaagtgg tggccattgc atctaatatc
     1681 ggggggaagc aagctcttga gacagttcaa cgtttgttac cagttctatg tcaagaccac
     1741 ggtcttacac ccgaccaggt agttgccatc gcatctcatg atggcggtaa gcaagcccta
     1801 gaaacggttc agagattgct gccggtactt tgccaggatc acgggttaac accggaccaa
     1861 gttgtcgcga ttgcctctaa tggtggagga aagcaggccc tggaatctat tgttgcacag
     1921 ctttcaagac cagatccggc tctggctgca ttgactaacg accatctggt ggcgttggca
     1981 tgtcttggtg gacgacccgc gctcgatgca gtcaaaaagg gtctgcctca tgctcccgca
     2041 ttgatcaaaa gaaccaaccg gcggattccc gagagaactt cccatcgagt cgcggatcac
     2101 gcgcaagtgg tccgcgtgct cggattcttc cagtgtcact cccaccccgc acaagcgttc
     2161 gatgacgcca tgactcaatt tggtatgtcg agacacggac tgctacagct ctttcgtaga
     2221 gtcggtgtca cagaactcga ggcccgctcg ggcacactgc ctcccgcctc ccagcggtgg
     2281 gacaggattc tccaagcgag cggtatgaaa cgcgcgaagc cttcacctac gtcaactcag
     2341 acacctgacc aggcgagcct tcatgcgttc gcagactcgc tggagaggga tttggacgcg
     2401 ccctcgccca tgcatgaagg ggaccaaact cgcgcgtcag ctagccccaa gaagaagaga
     2461 aaggtgggca gtggtgctca tattgtcatg gttgatgctt acaagccaac taaggagctc
     2521 tgagcatgtg ctctgtatgt atataaaact cttgttttct tcttttctct aaatattctt
     2581 tccttataca ttaggacctt tgcagcataa attactatac ttctattact agagcccgcc
     2641 gccaccatgg ag
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