LOCUS       BBa_K1861070            2657 bp    DNA     linear       30-Apr-2024
DEFINITION  Fusion protein of TALE3 and a SpyTag in an expression cassette
ACCESSION   BBa_K1861070
VERSION     BBa_K1861070.1  
FEATURES             Location/Qualifiers
     misc_feature    133..133
                     /label=T->C mutation to avoid SpeI restriction site
     promoter        1..238
                     /label=rpd3 promoter
     promoter        1..238
                     /label=BBa_K1861101
     misc_feature    239..241
                     /label=ATG start codon
     misc_feature    242..259
                     /label=6x His-Tag
     misc_feature    260..265
                     /label=BamHI restriction site
     misc_feature    239..265
                     /label=BBa_K1861102
     misc_feature    266..268
                     /label=start codon ATG
     CDS             266..724
                     /label=Front constant domain
     CDS             725..1960
                     /label=Variable domain
     CDS             1961..2476
                     /label=Back constant domain
     CDS             266..2480
                     /label=BBa_K1861701
     misc_feature    2481..2519
                     /label=SpyTag
     misc_feature    2499..2501
                     /label=Reactive Asp for isopeptide forming reaction
     CDS             2481..2519
                     /label=BBa_K1159201
     misc_feature    2520..2525
                     /label=SacI restriction site
     misc_feature    2526..2528
                     /label=TGA stop codon
     misc_feature    2520..2528
                     /label=BBa_K1861104
     terminator      2529..2657
                     /label=BBa_K392003
ORIGIN
        1 gtaatatcaa ctcagagcgt ataggtaaat ttgtaaatat atgtcccata ttttgccttg
       61 aaatttatct ttttatttac ttaccctccc cggctcatag tatctatcga aagggaaaaa
      121 cagaaaagat accagtagtt gattatacat aataaaaatt tgaagtaata accataaagg
      181 ttcataaaac aattgcgcca tacaaaacat tcgtggctac aactcgatat ccgtgcagat
      241 gcatcaccac catcatcacg gatccatggc ccccaagaag aagaggaagg tgggcattca
      301 ccgcggggta cctatggtgg acttgaggac actcggttat tcgcaacagc aacaggagaa
      361 aatcaagcct aaggtcagga gcaccgtcgc gcaacaccac gaggcgcttg tggggcatgg
      421 cttcactcat gcgcatattg tcgcgctttc acagcaccct gcggcgcttg ggacggtggc
      481 tgtcaaatac caagatatga ttgcggccct gcccgaagcc acgcacgagg caattgtagg
      541 ggtcggtaaa cagtggtcgg gagcgcgagc acttgaggcg ctgctgactg tggcgggtga
      601 gcttaggggg cctccgctcc agctcgacac cgggcagctg ctgaagatcg cgaagagagg
      661 gggagtaaca gcggtagagg cagtgcacgc ctggcgcaat gcgctcaccg gtgcccccct
      721 ccctaaattt gacgccggac caggtcgtag cgatagcttc ccatgacggg ggaaaacaag
      781 cgctggaaac agtacaaaga ttgctgccgg tcctttgtca agaccacggc cttactccgg
      841 atcaagttgt cgcaatcgca tctcacgatg ggggtaaaca ggcattagag acagttcaaa
      901 gactactgcc cgtcttgtgt caggatcacg gtctaactcc cgatcaagta gttgccatag
      961 cgtctaacca cggaggcaaa caggcgcttg agactgttca gcgtcttcta ccagtgcttt
     1021 gccaggacca tgggctgaca cccgaccagg ttgtggccat tgctagtaat ataggcggga
     1081 agcaggcgtt ggagactgtt caacgtttat tacccgtact atgccaagat catggcttga
     1141 ctcctgacca agtggtcgcc atcgcttctc atgatggagg aaagcaagca ttggaaactg
     1201 tccaaaggct tcttccggtc ttatgtcagg atcacggatt gacaccagat caggtggttg
     1261 ctattgcctc taacggcggt ggtaagcaag ccctagaaac cgtccagaga ttgttgccgg
     1321 tattcaagac cacgggttga ctcctgatca agtagtcgcc atagctagcc atgacggggg
     1381 caagcaagct ttagaaacag ttcagaggtt gctgccagtc ttatgtcagg atcacggttt
     1441 gaccccggac caggtggtcg ctattgcatc taatcacggg ggtaaacaag cactagaaac
     1501 tgtacaaagg ttgttgccgg tattatgcca ggaccatgga cttactcccg atcaagttgt
     1561 ggccatcgca tctcatgatg gtgggaaaca ggctttggaa accgttcaac gtcttttacc
     1621 agtgttgtgc caagatcatg ggctaacacc cgaccaagtg gtagcgattg cctctaatgg
     1681 tggcggcaaa caggccctgg aaacggttca gcgtttgtta cccgtactgt gtcaggatca
     1741 cggattaacg ccagaccagg ttgtagctat cgcttctaac catggcggta agcaggcgct
     1801 agagacagtc caaagactgc tgccggttct ttgtcaagac cacggtctta ccccagatca
     1861 ggtcgttgcc attgcgtcta acggaggtgg aaagcaagca ttggagtcta tcgttgctca
     1921 attatccaga ccagatccag cacttgctgc tttgactaac gaacgaccat ctggtggcgt
     1981 tggcatgtct tggtggacga cccgcgctcg atgcagtcaa aaagggtctg cctcatgctc
     2041 ccgcattgat caaaagaacc aaccggcgga ttcccgagag aacttcccat cgagtcgcgg
     2101 atcacgcgca agtggtccgc gtgctcggat tcttccagtg tcactcccac cccgcacaag
     2161 cgttcgatga cgccatgact caatttggta tgtcgagaca cggactgcta cagctctttc
     2221 gtagagtcgg tgtcacagaa ctcgaggccc gctcgggcac actgcctccc gcctcccagc
     2281 ggtgggacag gattctccaa gcgagcggta tgaaacgcgc gaagccttca cctacgtcaa
     2341 ctcagacacc tgaccaggcg agccttcatg cgttcgcaga ctcgctggag agggatttgg
     2401 acgcgccctc gcccatgcat gaaggggacc aaactcgcgc gtcagctagc cccaagaaga
     2461 agagaaaggt gggcagtggt gctcatattg tcatggttga tgcttacaag ccaactaagg
     2521 agctctgagc atgtgctctg tatgtatata aaactcttgt tttcttcttt tctctaaata
     2581 ttctttcctt atacattagg acctttgcag cataaattac tatacttcta ttactagagc
     2641 ccgccgccac catggag
//