| Types | DnaRegion
|
| Roles | CDS
Coding
|
| Sequences | BBa_K187015_sequence (Version 1)
|
Description
choramphenicol resistance can be used as a selectable marker on choramphenicol containing plates. This chloramphenicol resistance gene is present in plasmid pAB (part BBa_K187000), which is one of two universal plasmids for the BioBytes assembly method. For details of the BioBytes method, see its BBF RFC.
Notes
Note that the chlor resistance gene in this plasmid starts at the start codon and ends at the stop codon. A promoter and terminator are not included. pAB does include an RBS consensus sequence positioned 8 bp upstream of the ATG. We recommend that to express chlor resistance, you use the Biobytes assembly method together with the promoter and terminator parts we have submited in pAB and pBA to assemble promoters and terminators onto the chlor resistance gene. As there is a range of promoters to chose from, this allows rapid manipulation of gene expression level. Using the Biobytes method, several DNA segments can be combined in just 20min per segment.
Source
The choramphenicol resistance gene was cloned out of the plasmid pKD3. Biobytes plasmid pAB is entered as BBa_K187000.
