LOCUS       BBa_K1871002            1820 bp    DNA     linear       14-May-2024
DEFINITION  Alpha amylase with mating factor alpha secretion
ACCESSION   BBa_K1871002
VERSION     BBa_K1871002.1  
FEATURES             Location/Qualifiers
     promoter        1..104
                     /label=Minimal cyc Promoter
     RBS             105..125
                     /label=Kozak Sequence
     misc_feature    126..179
                     /label=Native Mating Factor Alpha Secretion Sequence
     CDS             180..1631
                     /label=Alpha Amylase Coding Sequence
     misc_feature    1632..1819
                     /label=ADH1 Terminator
ORIGIN
        1 ggcatgtgct ctgtatgtat ataaaactct tgttttcttc ttttctctaa atattctttc
       61 cttatacatt aggacctttg cagcataaat tactatactt ctatggatcc acgattaaaa
      121 gaatgagatt cccatccatt ttcaccgctg ttttgttcgc cgcttcttct gctttggctg
      181 taaatggcac gctgatgcag tattttgaat ggtatacgcc gaacgacggc cagcattgga
      241 aacgattgca gaatgatgcg gaacatttat cggatatcgg aatcactgcc gtctggattc
      301 ctcccgcata caaaggattg agccaatccg ataacggata cggaccttat gatttgtatg
      361 atttaggaga gttccagcaa aaagggacgg tcagaacgaa atacggcaca aaatcagagc
      421 ttcaagatgc gatcggctca ctgcattccc ggaacgtcca agtatacgga gatgtggttt
      481 tgaatcataa ggctggtgct gatgcaacag aagatgtaac tgccgtcgaa gtcaatccgg
      541 ccaatagaaa tcaggaaact tcggaggaat atcaaatcaa agcgtggacg gattttcgtt
      601 ttccgggccg tggaaacacg tacagtgatt ttaaatggca ttggtatcat ttcgacggag
      661 cggactggga tgaatcccgg aagatcagcc gcatctttaa gtttcgtggg gaaggaaaag
      721 cgtgggattg ggaagtatca agtgaaaacg gcaactatga ctatttaatg tatgctgatg
      781 ttgactacga ccaccctgat gtcgtggcag agacaaaaaa atggggtatc tggtatgcga
      841 atgaactgtc attagacggc ttccgtattg atgccgccaa acatattaaa ttttcatttc
      901 tgcgtgattg ggttcaggcg gtcagacagg cgacgggaaa agaaatgttt acggttgcgg
      961 agtattggca gaataatgcc gggaaactcg aaaactactt gaataaaaca agctttaatc
     1021 aatccgtgtt tgatgttccg cttcatttca atttacaggc ggcttcctca caaggaggcg
     1081 gatatgatat gaggcgtttg ctggacggta ccgttgtgtc caggcatccg gaaaaggcgg
     1141 ttacatttgt tgaaaatcat gacacacagc cgggacagtc attggaatcg acagtccaaa
     1201 cttggtttaa accgcttgca tacgccttta ttttgacaag agaatccggt tatcctcagg
     1261 tgttctatgg ggatatgtac gggacaaaag ggacatcgcc aaaggaaatt ccctcactga
     1321 aagataatat agagccgatt ttaaaagcgc gtaaggagta cgcatacggg ccccagcacg
     1381 attatattga ccacccggat gtgatcggat ggacgaggga aggtgacagc tccgccgcca
     1441 aatcaggttt ggccgcttta atcacggacg gacccggcgg atcaaagcgg atgtatgccg
     1501 gcctgaaaaa tgccggcgag acatggtatg acataacggg caaccgttca gatactgtaa
     1561 aaatcggatc tgacggctgg ggagagtttc atgtaaacga tgggtccgtc tccatttatg
     1621 ttcagaaata agcgaatttc ttatgattta tgatttttat tattaaataa gttataaaaa
     1681 aaataagtgt atacaaattt taaagtgact cttaggtttt aaaacgaaaa ttcttattct
     1741 tgagtaactc tttcctgtag gtcaggttgc tttctcaggt atagtatgag gtcgctctta
     1801 ttgaccacac ctctaccggt
//