BBa_K1879001 1 BBa_K1879001 promoter + CBZ cleavage enzyme + terminator 2016-10-11T11:00:00Z 2016-10-12T09:46:00Z The coding sequence of this CBZ cleavage enzyme came from a study by Patel et al. See Part:BBa_K1879000, which is just the enzyme coding sequence. The promoter (BBa_J23118) and terminator (BBa_J61048) came from the iGEM kit. This part contains the CBZ-cleavage enzyme with a promoter (BBa_J23118) and RBS upstream of the coding sequence, and terminator (BBa_J61048) downstream of the sequence to make a fully functioning BioBrick. This BioBrick is used in our biocontainment system to cleave the CBZ protecting group from leucine prior to incorporation of leucine into proteins. This part can be used by other iGEM teams to selectively cleave the CBZ protecting group from amino or hydroxyl groups in organic reactions. false false _2344_ 29574 29574 9 false The promoter and terminator were cloned into the vector containing the cleavage enzyme. false Madeleine Stone component2495838 1 BBa_J61048 component2495836 1 BBa_J23118 component2495837 1 BBa_K1879000 annotation2495837 1 BBa_K1879000 range2495837 1 44 1394 annotation2495838 1 BBa_J61048 range2495838 1 1403 1515 annotation2495836 1 BBa_J23118 range2495836 1 1 35 BBa_J61048 1 BBa_J61048 [rnpB-T1] Terminator 2007-02-20T12:00:00Z 2015-08-31T02:03:00Z bob bob false false _95_ 0 483 95 In stock false bob true John Anderson BBa_K1879000 1 BBa_K1879000 CDS of N-CBZ cleavage enzyme 2016-10-11T11:00:00Z 2016-10-12T09:45:51Z The protein sequence was reported in a study conducted by Patel et al (1). 1. Nanduri, V. B., Goldberg, S., Johnston, R. & Patel, R. N. Cloning and expression of a novel enantioselective N-carbobenzyloxy-cleaving enzyme. Enzyme and Microbial Technology 34, 304???312 (2004). This part contains just the coding sequence for the N-carbobenzyloxy (CBZ)-cleavage enzyme necessary to enantioselectively remove the CBZ protecting group from amino acids. In our biocontainment system, the enzyme serves to cleave CBZ from leucyl-tRNA charged with CBZ-protected leucine. This part can be used by other iGEM teams to selectively cleave the CBZ protecting group from amino or hydroxyl groups in organic reactions. false false _2344_ 29574 29574 9 false The DNA sequence was found in a study by Patel et al. In order to remove forbidden restriction sites from the coding sequence, silent mutations were made to remove the restriction site while preserving the amino acid sequence. The piece of DNA was synthesized commercially. false Madeleine Stone BBa_J23118 1 BBa_J23118 constitutive promoter family member 2006-08-16T11:00:00Z 2015-08-31T04:08:40Z Later Released HQ 2013 Later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_J23118_sequence 1 ttgacggctagctcagtcctaggtattgtgctagc BBa_J61048_sequence 1 ccggcttatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtccacgacgctatacccaaaagaaa BBa_K1879001_sequence 1 ttgacggctagctcagtcctaggtattgtgctagctactagagctagagagaaaaggtctcaatgcggtagataaaggaccactggctgcatggtgcaaccaacgccaacacctcaatccgaacttccgggcctgattgcgcgtgatatggaaggtttaatgactttatatcgtgacttgcatgctaatccagaattgagcttacaagaagttaacacagcggccaagctggcaaaacgtctgaaagcaatgaaatttgacgtcaccgagaaagtgggtggcacaggtgtggtcgctgttatgaagaacggctcaggcccagttttgttgattcgtgcagatatggacggtttaccggtagtagaacagacaggcttagacttcgcgtccaaggtccgtactaaaactccggagggggtggagacgggcgtcatgcatgcctgcgggcacgatactcatatgactgcttttattgaaaccgccaagctgttgtcttcccaaaaagacaagtggaagggaacactggttatgatcttacaacccgccgaggaagtaggcaaaggcgctcgcgatatgttagaggacgggttatacacacgttttcctcgtccgacccacgcgattgcatttcacgacgctgccaacctacaggctggggtcgtcgggtataccccagggtatgctctggcgaacgttgacagtgttgacatcgtagtgaaagggttgggtggacacggagcgtatcctcaaacaacgcgtgatcccattgtattaggtagccgcattgtgacgtcgttacaaacccttgtcagccgcgagcaagacccgcaagaccctgctgtagtgactgtcggtagtttccaagcaggggcaaagcataacatcatcccagaccaggccttgcttttattgactgtccgttcgtactccgacgaaacacgtgcgaaattgatcaaaggaatcgaacgtatcgcgcgtggggaggcaatcgcagcaggtgtgcctgatgacaagatgccagtggttagcgtcaaagatgagttcacaccatcaacatacaatccacctgaatttgcagagcaaatgggagcattactgaaggggcattttgcagagggacgcgttgttaaaacacccgcagtgatggggggcgaagacttcggacgcttttaccgcgccgataagtcaatcaactcgttcattttctgggtcggcggagtgcctgcggataagatggccgctgctcaagcaggacagatcacgctgcctagcttacattcaccattttgggctcccgaggcggataaagttattgctactgcctcggaagcgatgacggtgttagccatggacatcctgaaaaaagattgaatgcagagaccttggatactagatactagagccggcttatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtccacgacgctatacccaaaagaaa BBa_K1879000_sequence 1 ctagagagaaaaggtctcaatgcggtagataaaggaccactggctgcatggtgcaaccaacgccaacacctcaatccgaacttccgggcctgattgcgcgtgatatggaaggtttaatgactttatatcgtgacttgcatgctaatccagaattgagcttacaagaagttaacacagcggccaagctggcaaaacgtctgaaagcaatgaaatttgacgtcaccgagaaagtgggtggcacaggtgtggtcgctgttatgaagaacggctcaggcccagttttgttgattcgtgcagatatggacggtttaccggtagtagaacagacaggcttagacttcgcgtccaaggtccgtactaaaactccggagggggtggagacgggcgtcatgcatgcctgcgggcacgatactcatatgactgcttttattgaaaccgccaagctgttgtcttcccaaaaagacaagtggaagggaacactggttatgatcttacaacccgccgaggaagtaggcaaaggcgctcgcgatatgttagaggacgggttatacacacgttttcctcgtccgacccacgcgattgcatttcacgacgctgccaacctacaggctggggtcgtcgggtataccccagggtatgctctggcgaacgttgacagtgttgacatcgtagtgaaagggttgggtggacacggagcgtatcctcaaacaacgcgtgatcccattgtattaggtagccgcattgtgacgtcgttacaaacccttgtcagccgcgagcaagacccgcaagaccctgctgtagtgactgtcggtagtttccaagcaggggcaaagcataacatcatcccagaccaggccttgcttttattgactgtccgttcgtactccgacgaaacacgtgcgaaattgatcaaaggaatcgaacgtatcgcgcgtggggaggcaatcgcagcaggtgtgcctgatgacaagatgccagtggttagcgtcaaagatgagttcacaccatcaacatacaatccacctgaatttgcagagcaaatgggagcattactgaaggggcattttgcagagggacgcgttgttaaaacacccgcagtgatggggggcgaagacttcggacgcttttaccgcgccgataagtcaatcaactcgttcattttctgggtcggcggagtgcctgcggataagatggccgctgctcaagcaggacagatcacgctgcctagcttacattcaccattttgggctcccgaggcggataaagttattgctactgcctcggaagcgatgacggtgttagccatggacatcctgaaaaaagattgaatgcagagaccttggatactaga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z