LOCUS       BBa_K188161             2142 bp    DNA     linear       19-May-2024
DEFINITION  constitutive promoter +RBS+LacI+RBS+luxR+Terminator
ACCESSION   BBa_K188161
VERSION     BBa_K188161.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23106
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    1151..1189
                     /label=LVA
     CDS             62..1189
                     /label=lacI-LVA
     misc_feature    62..1189
                     /label=BBa_C0012
     misc_feature    1190..1214
                     /label=Help:Barcodes
     CDS             62..1189
                     /label=BBa_C0012
     misc_feature    1227..1230
                     /label=conserved
     RBS             1223..1234
                     /label=BBa_B0034
     misc_feature    1241..1242
                     /label=prefix
     misc_feature    1414..1414
                     /label=T
     misc_feature    1732..1732
                     /label=A
     CDS             1241..1990
                     /label=luxR
     misc_feature    1241..1996
                     /label=BBa_C0062
     misc_feature    1997..2021
                     /label=Help:Barcodes
     CDS             1241..1996
                     /label=BBa_C0062
     terminator      2030..2142
                     /label=BBa_J61048
ORIGIN
        1 tttacggcta gctcagtcct aggtatagtg ctagctacta gagaaagagg agaaatacta
       61 gatggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta
      121 tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa
      181 agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc
      241 gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc
      301 gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc
      361 gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca
      421 acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga
      481 agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa
      541 cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt
      601 gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg
      661 tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga
      721 aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat
      781 cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat
      841 taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga
      901 agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg
      961 gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca
     1021 gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc
     1081 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     1141 aagcgggcag gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg
     1201 ctagtgtaga tctctactag agaaagagga gaaatactag atgaaaaaca taaatgccga
     1261 cgacacatac agaataatta ataaaattaa agcttgtaga agcaataatg atattaatca
     1321 atgcttatct gatatgacta aaatggtaca ttgtgaatat tatttactcg cgatcattta
     1381 tcctcattct atggttaaat ctgatatttc aatcctagat aattacccta aaaaatggag
     1441 gcaatattat gatgacgcta atttaataaa atatgatcct atagtagatt attctaactc
     1501 caatcattca ccaattaatt ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc
     1561 aaatgtaatt aaagaagcga aaacatcagg tcttatcact gggtttagtt tccctattca
     1621 tacggctaac aatggcttcg gaatgcttag ttttgcacat tcagaaaaag acaactatat
     1681 agatagttta tttttacatg cgtgtatgaa cataccatta attgttcctt ctctagttga
     1741 taattatcga aaaataaata tagcaaataa taaatcaaac aacgatttaa ccaaaagaga
     1801 aaaagaatgt ttagcgtggg catgcgaagg aaaaagctct tgggatattt caaaaatatt
     1861 aggttgcagt gagcgtactg tcactttcca tttaaccaat gcgcaaatga aactcaatac
     1921 aacaaaccgc tgccaaagta tttctaaagc aattttaaca ggagcaattg attgcccata
     1981 ctttaaaaat taataacact gatagtgcta gtgtagatca ctactagagc cggcttatcg
     2041 gtcagtttca cctgatttac gtaaaaaccc gcttcggcgg gtttttgctt ttggaggggc
     2101 agaaagatga atgactgtcc acgacgctat acccaaaaga aa
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