LOCUS       BBa_K188281             2392 bp    DNA     linear       28-May-2024
DEFINITION  P lacI  with  CI434  tetR and  GFP
ACCESSION   BBa_K188281
VERSION     BBa_K188281.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             82..750
                     /label=cI 434
     misc_feature    712..750
                     /label=LVA
     misc_feature    82..750
                     /label=BBa_C0052
     misc_feature    751..775
                     /label=Help:Barcodes
     CDS             82..750
                     /label=BBa_C0052
     misc_feature    788..791
                     /label=conserved
     RBS             784..795
                     /label=BBa_B0034
     CDS             805..1421
                     /label=tetR
     misc_feature    1422..1455
                     /label=SsrA
     misc_feature    1462..1486
                     /label=Help:Barcodes
     CDS             802..1486
                     /label=BBa_C0040
     misc_feature    1499..1502
                     /label=conserved
     RBS             1495..1506
                     /label=BBa_B0034
     misc_feature    1513..1515
                     /label=start
     misc_feature    2266..2268
                     /label=stop
     misc_feature    2269..2271
                     /label=stop
     misc_feature    2229..2265
                     /label=LVA tag
     CDS             1516..2228
                     /label=cds
     misc_feature    1513..2271
                     /label=BBa_K145015
     terminator      2280..2392
                     /label=BBa_J61048
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgagtatt tcttccaggg taaaaagcaa aagaatccag
      121 cttggactta accaggctga acttgctcaa aaggtgggga ctacccagca gtctatagag
      181 cagctcgaaa acggtaaaac taagcgacca cgctttttac cagaacttgc gtcagctctt
      241 ggcgtaagtg ttgactggct gctcaatggc acctctgatt cgaatgttag atttgttggg
      301 cacgttgagc ccaaagggaa atatccattg attagcatgg ttagagctgg ttcgtggtgt
      361 gaagcttgtg aaccctacga tatcaaggac attgatgaat ggtatgacag tgacgttaac
      421 ttattaggca atggattctg gctgaaggtt gaaggtgatt ccatgacctc acctgtaggt
      481 caaagcatcc ctgaaggtca tatggtgtta gtagatactg gacgggagcc agtgaatgga
      541 agccttgttg tagccaaact gactgacgcg aacgaagcaa cattcaagaa actggtcata
      601 gatggcggtc agaagtacct gaaaggcctg aatccttcat ggcctatgac tcctatcaac
      661 ggaaactgca agattatcgg tgttgtcgtg gaagcgaggg taaaattcgt agctgcaaac
      721 gacgaaaact acgctttagt agcttaataa ctctgatagt gctagtgtag atctctacta
      781 gagaaagagg agaaatacta gatgtccaga ttagataaaa gtaaagtgat taacagcgca
      841 ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag
      901 ctaggtgtag agcagcctac attgtattgg catgtaaaaa ataagcgggc tttgctcgac
      961 gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt agaaggggaa
     1021 agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt actaagtcat
     1081 cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta tgaaactctc
     1141 gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc attatatgca
     1201 ctcagcgctg tggggcattt tactttaggt tgcgtattgg aagatcaaga gcatcaagtc
     1261 gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt acgacaagct
     1321 atcgaattat ttgatcacca aggtgcagag ccagccttct tattcggcct tgaattgatc
     1381 atatgcggat tagaaaaaca acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac
     1441 tacgctttag tagcttaata acactgatag tgctagtgta gatcactact agagaaagag
     1501 gagaaatact agatgcgtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt
     1561 gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat
     1621 gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca
     1681 tggccaacac ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat
     1741 catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga
     1801 actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt
     1861 gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt
     1921 cttggacaca aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa
     1981 caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt
     2041 caactagcag accattatca acaaaatact ccaattggcg atggccctgt ccttttacca
     2101 gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac
     2161 cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta
     2221 tacaaaaggc ctgctgcaaa cgacgaaaac tacgctttag tagcttaata atactagagc
     2281 cggcttatcg gtcagtttca cctgatttac gtaaaaaccc gcttcggcgg gtttttgctt
     2341 ttggaggggc agaaagatga atgactgtcc acgacgctat acccaaaaga aa
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