LOCUS       BBa_K188343              942 bp    DNA     linear       14-Jun-2024
DEFINITION  Ptet+RBS+GFP(LVA)+terminator
ACCESSION   BBa_K188343
VERSION     BBa_K188343.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..83
                     /label=start
     misc_feature    834..836
                     /label=stop
     misc_feature    837..839
                     /label=stop
     misc_feature    797..833
                     /label=LVA tag
     CDS             84..796
                     /label=cds
     misc_feature    81..839
                     /label=BBa_K145015
     stem_loop       855..874
                     /label=T7 TE
     misc_feature    881..881
                     /label=stop
     polyA_site      875..888
                     /label=polya
     misc_feature    848..888
                     /label=BBa_B0012
     terminator      848..888
                     /label=BBa_B0012
     stem_loop       909..931
                     /label=stem_loop
     misc_feature    897..942
                     /label=BBa_B0011
     terminator      897..942
                     /label=BBa_B0011
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgcgtaaag gagaagaact tttcactgga gttgtcccaa
      121 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg
      181 aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac
      241 ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat
      301 acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac
      361 aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt
      421 ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg
      481 gaaacattct tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg
      541 cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg
      601 gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc
      661 ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa
      721 agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg
      781 atgaactata caaaaggcct gctgcaaacg acgaaaacta cgctttagta gcttaataat
      841 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagagag
      901 aatataaaaa gccagattat taatccggct tttttattat tt
//