LOCUS       BBa_K1885000            1155 bp    DNA     linear       13-Jun-2024
DEFINITION  PETase >PSB1C3
ACCESSION   BBa_K1885000
VERSION     BBa_K1885000.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     CDS             44..131
                     /label=end of LacI coding region (inactive)
     misc_feature    44..243
                     /label=BBa_R0010
     misc_feature    132..169
                     /label=CAP binding site
     promoter        180..185
                     /label=-35
     promoter        204..209
                     /label=-10
     misc_feature    209..243
                     /label=LacI binding site
     misc_feature    216..216
                     /label=start
     promoter        44..243
                     /label=BBa_R0010
     misc_feature    256..259
                     /label=conserved
     RBS             252..263
                     /label=BBa_B0034
     CDS             270..944
                     /label=mrfp1
     misc_feature    951..975
                     /label=Help:Barcodes
     CDS             270..950
                     /label=BBa_E1010
     stem_loop       995..1038
                     /label=stem_loop
     misc_feature    984..1063
                     /label=BBa_B0010
     terminator      984..1063
                     /label=BBa_B0010
     stem_loop       1079..1098
                     /label=T7 TE
     misc_feature    1105..1105
                     /label=stop
     polyA_site      1099..1112
                     /label=polya
     misc_feature    1072..1112
                     /label=BBa_B0012
     terminator      1072..1112
                     /label=BBa_B0012
     misc_feature    44..1112
                     /label=BBa_J04450
     promoter        1121..1155
                     /label=BBa_J23100
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagcaatacg caaaccgcct
       61 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
      121 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
      181 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
      241 acatactaga gaaagaggag aaatactaga tggcttcctc cgaagacgtt atcaaagagt
      301 tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg tcacgagttc gaaatcgaag
      361 gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc taaactgaaa gttaccaaag
      421 gtggtccgct gccgttcgct tgggacatcc tgtccccgca gttccagtac ggttccaaag
      481 cttacgttaa acacccggct gacatcccgg actacctgaa actgtccttc ccggaaggtt
      541 tcaaatggga acgtgttatg aacttcgaag acggtggtgt tgttaccgtt acccaggact
      601 cctccctgca agacggtgag ttcatctaca aagttaaact gcgtggtacc aacttcccgt
      661 ccgacggtcc ggttatgcag aaaaaaacca tgggttggga agcttccacc gaacgtatgt
      721 acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg tctgaaactg aaagacggtg
      781 gtcactacga cgctgaagtt aaaaccacct acatggctaa aaaaccggtt cagctgccgg
      841 gtgcttacaa aaccgacatc aaactggaca tcacctccca caacgaagac tacaccatcg
      901 ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg tgcttaataa cgctgatagt
      961 gctagtgtag atcgctacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     1021 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
     1081 ctcaccttcg ggtgggcctt tctgcgttta tatactagag ttgacggcta gctcagtcct
     1141 aggtacagtg ctagc
//