LOCUS BBa_K1886015 3562 bp DNA linear 01-Jun-2024 DEFINITION AND GATE-input_green light+IPTG ACCESSION BBa_K1886015 VERSION BBa_K1886015.1 FEATURES Location/Qualifiers promoter 1..238 /label=PcpcG2 promoter promoter 1..238 /label=BBa_K592003 misc_feature 247..382 /label=BBa_K228001 stem_loop 402..445 /label=stem_loop misc_feature 391..470 /label=BBa_B0010 terminator 391..470 /label=BBa_B0010 stem_loop 486..505 /label=T7 TE misc_feature 512..512 /label=stop polyA_site 506..519 /label=polya misc_feature 479..519 /label=BBa_B0012 terminator 479..519 /label=BBa_B0012 terminator 391..519 /label=BBa_B0015 CDS 528..615 /label=end of LacI coding region (inactive) misc_feature 528..727 /label=BBa_R0010 misc_feature 616..653 /label=CAP binding site promoter 664..669 /label=-35 promoter 688..693 /label=-10 misc_feature 693..727 /label=LacI binding site misc_feature 700..700 /label=start promoter 528..727 /label=BBa_R0010 RBS 736..746 /label=RBS-4\Weaker RBS 742..745 /label=RBS misc_feature 736..746 /label=BBa_B0033 RBS 736..746 /label=BBa_B0033 CDS 753..3425 /label=cds CDS 753..3425 /label=BBa_K228000 stem_loop 3445..3488 /label=stem_loop misc_feature 3434..3513 /label=BBa_B0010 terminator 3434..3513 /label=BBa_B0010 stem_loop 3529..3548 /label=T7 TE misc_feature 3555..3555 /label=stop polyA_site 3549..3562 /label=polya misc_feature 3522..3562 /label=BBa_B0012 terminator 3522..3562 /label=BBa_B0012 terminator 3434..3562 /label=BBa_B0015 ORIGIN 1 agcccattgt gcttttctct atcaacctca gcttacctga aggggtgaac aggtctgggt 61 taattcatgt tgcgaaatgt aacagtttta gtcgcatcag ctaactttcc gatttcttta 121 cgattttctc ccccttttct tcaattttac tttgttagga tcgcattttt aatgccaaca 181 cataccagtt attggctgga cattaaacaa cttttaagtt taattactaa ctttatctta 241 ctagagcaat tcggagagat gccggagcgg ctgaacggac cggtctctaa aaccggagta 301 ggggcaactc taccgggggt tcaaatcccc ctctctccgc cactacagat ccttagcgaa 361 agctaaggat tttttttaag cttactagag ccaggcatca aataaaacga aaggctcagt 421 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 481 acactggctc accttcgggt gggcctttct gcgtttatat actagagcaa tacgcaaacc 541 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg 601 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca 661 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt 721 tcacacatac tagagtcaca caggactact agatgaccat gattaccgtg cactagaata 781 ccattaacat tgctaagaac gacttctctg acatcgaact ggctgctatc ccgttcaaca 841 ctctggctga ccattacggt gagcgtttag ctcgcgaaca gttggccctt gagcatgagt 901 cttacgagat gggtgaagca cgcttccgca agatgtttga gcgtcaactt aaagctggtg 961 aggttgcgga taacgctgcc gccaagcctc tcatcactac cctactccct aagatgattg 1021 cacgcatcaa cgactggttt gaggaagtga aagctaagcg cggcaagcgc ccgacagcct 1081 tccagttcct gtaggaaatc aagccggaag ccgtagcgta catcaccatt aagaccactc 1141 tggcttgcct aaccagtgct gacaatacaa ccgttcaggc tgtagcaagc gcaatcggtc 1201 gggccattga ggacgaggct cgcttcggtc gtatccgtga ccttgaagct aagcacttca 1261 agaaaaacgt tgaggaacaa ctcaacaagc gcgtagggca cgtctacaag aaagcattta 1321 tgcaagttgt cgaggctgac atgctctcta agggtctact cggtggcgag gcgtggtctt 1381 cgtggcataa ggaagactct attcatgtag gagtacgctg catcgagatg ctcattgagt 1441 caaccggaat ggttagctta caccgccaaa atgctggcgt agtaggtcaa gactctgaga 1501 ctatcgaact cgcacctgaa tacgctgagg ctatcgcaac ccgtgcaggt gcgctggctg 1561 gcatctctcc gatgttccaa ccttgcgtag ttcctcctaa gccgtggact ggcattactg 1621 gtggtggcta ttgggctaac ggtcgtcgtc ctctggcgct ggtgcgtact cacagtaaga 1681 aagcactgat gcgctacgaa gacgtttaca tgcctgaggt gtacaaagcg attaacattg 1741 cgcaaaacac cgcatggaaa atcaacaaga aagtcctagc ggtcgccaac gtaatcacca 1801 agtggaagca ttgtccggtc gaggacatcc ctgcgattga gcgtgaagaa ctcccgatga 1861 aaccggaaga catcgacatg aatcctgagg ctctcaccgc gtggaaacgt gctgccgctg 1921 ctgtgtaccg caaggacaag gctcgcaagt ctcgccgtat cagccttgag ttcatgcttg 1981 agcaagccaa taagtttgct aaccataagg ccatctggtt cccttacaac atggactggc 2041 gcggtcgtgt ttacgctgtg tcaatgttca acccgcaagg taacgatatg accaaaggac 2101 tgcttacgct ggcgaaaggt aaaccaatcg gtaaggaagg ttactactgg ctgaaaatcc 2161 acggtgcaaa ctgtgcgggt gtcgataagg ttccgttccc tgagcgcatc aagttcattg 2221 aggaaaacca cgagaacatc atggcttgcg ctaagtctcc actggagaac acttggtggg 2281 ctgagcaaga ttctccgttc tgcttccttg cgttctgctt tgagtacgct ggggtacagc 2341 accacggcct gagctataac tgctcccttc cgctggcgtt tgacgggtct tgctctggca 2401 tccagcactt ctccgcgatg ctccgagatg aggtaggtgg tcgcgcggtt aacttgcttc 2461 ctagtgaaac cgttcaggac atctacggga ttgttgctaa gaaagtcaac gagattctac 2521 aagcagacgc aatcaatggg accgataacg aagtagttac cgtgaccgat gagaacactg 2581 gtgaaatctc tgagaaagtc aagctgggca ctaaggcact ggctggtcaa tggctggctt 2641 acggtgttac tcgcagtgtg actaagcgtt cagtcatgac gctggcttac gggtccaaag 2701 agttcggctt ccgtcaacaa gtgctggaag ataccattca gccagctatt gattccggca 2761 agggtctgat gttcactcag ccgaatcagg ctgctggata catggctaag ctgatttggg 2821 aatctgtgag cgtgacggtg gtagctgcgg ttgaagcaat gaactggctt aagtctgctg 2881 ctaagctgct ggctgctgag gtcaaagata agaagactgg agagattctt cgcaagcgtt 2941 gcgctgtgca ttgggtaact cctgatggtt tccctgtgtg gcaggaatac aagaagccta 3001 ttcagacgcg cttgaacctg atgttcctcg gtcagttccg cttacagcct accattaaca 3061 ccaacaaaga tagcgagatt gatgcacaca aacaggagtc tggtatcgct cctaactttg 3121 tacacagcca agacggtagc caccttcgta agactgtagt gtgggcacac gagaagtacg 3181 gaatcgaatc ttttgcactg attcacgact ccttcggtac cattccggct gacgctgcga 3241 acctgttcaa agcagtgcgc gaaactatgg ttgacacata tgagtcttgt gatgtactgg 3301 ctgatttcta cgaccagttc gctgaccagt tgcacgagtc tcaattggac aaaatgccag 3361 cacttccggc taaaggtaac ttgaacctcc gtgacatctt agagtcggac ttcgcgttcg 3421 cataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt 3481 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg 3541 ggtgggcctt tctgcgttta ta //