LOCUS       BBa_K1890031             519 bp    DNA     linear       02-Jun-2024
DEFINITION  BolA gene with strong constitutive promoter, RBS and terminator
ACCESSION   BBa_K1890031
VERSION     BBa_K1890031.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    1..55
                     /label=BBa_S03952
     CDS             62..382
                     /label=BBa_K1890056
     stem_loop       402..445
                     /label=stem_loop
     misc_feature    391..470
                     /label=BBa_B0010
     terminator      391..470
                     /label=BBa_B0010
     stem_loop       486..505
                     /label=T7 TE
     misc_feature    512..512
                     /label=stop
     polyA_site      506..519
                     /label=polya
     misc_feature    479..519
                     /label=BBa_B0012
     terminator      479..519
                     /label=BBa_B0012
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatgatgata cgtgagcgga tagaagaaaa attaagggcg gcgttccaac ccgtattcct
      121 cgaagtagtg gatgaaagct atcgtcacaa tgtcccagcc ggctctgaaa gccattttaa
      181 agttgtgctg gtcagcgatc gttttacggg tgaacgtttt ctgaatcgtc atcgaatgat
      241 ttacagtact ttagcggagg aactctctac taccgttcat gcgctggctc tgcatactta
      301 cactattaag gagtgggaag ggttgcagga caccgtcttt gcctctcctc cctgtcgtgg
      361 agcaggaagc atcgcgtaat aatactagag ccaggcatca aataaaacga aaggctcagt
      421 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
      481 acactggctc accttcgggt gggcctttct gcgtttata
//