BBa_K1891002 1 BBa_K1891002 Luciferase 2016-10-09T11:00:00Z 2016-10-10T01:25:38Z We get this gene sequence from NCBI (Genebank:M65067.1), and then we get the DNA fragment from a synthesis company. It encodes whole luciferase under the control of pBAD promoter. And we add arabinose until its final concentration is 1mmol/L, incubate for 6h(37??C, 250rpm). We can detect its activity by add its substrate fluorescein. false false _2356_ 27040 27040 9 false The DNA sequence of luciferase gene is from NCBI (Genebank:M65067.1). A pBAD promoter(BBa_K206000) and a RBS(BBa_B0034) are added upstream the functional gene to produce this enzyme. Lociferase-pSB1C3 plasmid is transformed into E. coli strain transB (DE3) to express the protein. It can show fluorescence when we add its substrate fluorescein. false Zhao Jingyu annotation2489637 1 luciferase range2489637 1 143 2282 annotation2489635 1 BBa_K206000 range2489635 1 1 130 annotation2489638 1 BBa_B0015 range2489638 1 2283 2411 annotation2489636 1 BBa_B0034 range2489636 1 131 142 BBa_K1891002_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagcaaagaggagaaataattacaactgatacataaaccaacaaaaggaatattatgaaatttggtaatatctttagctatcagccgccgggcgaaagccacaaagaagtgatggaccgcttcgttcgcctgggcgtggcaagcgaggaactgaatttcgacacctactggctggagcaccactttaccgaatttggtctgaccggcaacctgttcgtggcatgcgccaacctgctgggccgtaccaccaagctgaatgtgggcaccatgatcgtgctgccgaccgcacatccggcacgccagatggaagatctgctgctgctggatcagatgagcaagggccgcttcaacttcggtgttgttcgcggctatcacaaggattttcgcgtgttcggcgtgaccatggaagatagccgtgccatcaccgaagactttcataccatgattatggatggcaccaagaccggtctgcataccgacggcaagaacatcgagtttccggacgtgaacgtgtatcctgaggcctacctggagaagatcccgacctgtatgaccgccgaaagcgccaccaccacctggttagccgagcgtggcctgcctatggtgctgagctggatcatcaccaccagcgagaaaaaggcccagatggagctgtacaatgccgtgcgcgacagcggttacagtgaagaatacatcaaaaacgtggaccacagcatgaccctgatttgttctgtggatgaggacggtaagaaagccgaagacgttcgcgagttcctgggcaattggtacgacagctatgtgaacgccaccaacatcttcagcgagagcaaccagacccgcggttacgactaccacaaaggccaaaaggacttcgtgttacaaggccataccaacaccaagcgtcgcgtggattacagcaatgacctgaatccggtgggcacccctgagaagtgcatcgagattcagcgcgacatcgacgccaccggcattaccaacatcaccctgggtttcgaagccaatggcagcgaggaagagattatcgccagcatgaagcgcttcatgcaggttgccccgtttctgaaggatccgaaataaataaatcactcagattaactttaataaataatataaggaatataacatgaattttggcctgttttttctgaatttcccggagaacaccagcagcgaaaccgtgctggacaacatgatcaacaccgtgagcctggtggataaagactataaaaattttaccaccgcattagtgaatcatcactttagcaaaaatggtatcgtgggcgccccgatgaccgcagcaagctttctgctgggcctgaccgaacgcctgcatatcggtagcctgaaccagatcaccacccatcatccggtgcgcattgccgaggaagccagcctgctggatcagatgagcgacagccgcttcattctgggcttaagcgattgcgtgaattttgagatggacttttttaagcgccagcgcgatagccagcagttacaatttgaggcctgctacgacatcatcaacgaagccatcaccaccaactactgcgccaataatgatttttataacttcccgcgtattagcattaacccgcactgcctgagcaaggagaacatgaagcagtacatcctggccagcagcgttagcgtggaatgggccgccaaaaaagccctgccgctgacctaccgttggagtgataccctggaggacaaggagatcctgtacaaacgctacctggaggtggccaaacacaacatcgatgtgagcaacgttgaacatcagttccctctgctggtgaacctgaatcatgatcgcgacgttgcacaccaagaagctaccgcctacgtgagctacattgccgaagtttacccgcacctgaaccagcagcaaaagatcgccgagctgattagccaacacgccattggcaccgataacgattactattctaccctgaatgcactggagcgcaccggcagcaaaaacgtgctgctgagcttcgagagcatgaagaaccatgatgatgtggtgaaggtgattaatatgaatgaaaaaattcagaaaaatctgccgagtagctaagtgtaaaggaagcggtgttattatgaataaatggaattacggagtcttcttcgttaacccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z