BBa_K1891005 1 BBa_K1891005 alpha-tubulin+N-Luciferase 2016-10-09T11:00:00Z 2016-10-10T08:58:12Z Alpha-tubulin comes from human breast cancer cell line Mcf-7 cDNA using PCR. N-Luciferase comes from pCambia1300-N-Luciferase plasmid. This part expresses alpha-tubulin- N-Luciferase fusion protein . The C-terminal of alpha-tubulin links to the N-terminal of N-Luciferase. We connected this two gene fragment using fusion PCR. And then we cloned it into pET30a(+) and add IPTG until its final concentration is 1mmol/L, incubate for 4-6h(37??C, 250rpm) to induce the expression of this fusion protein. false false _2356_ 27040 27040 9 false We connected this two gene fragments using fusion PCR. First we acquired alpha-tubulin gene fragment and N-Luciferase gene fragment. And then we redesigned a antisense primer of alpha-tubulin and a sense primer of N-Luciferase with a linker about 42bp. And then doing fusion PCR. false Zhao Jingyu annotation2492727 1 alpha-tubulin range2492727 1 1 1356 annotation2492750 1 linker range2492750 1 1357 1398 annotation2492728 1 N-luciferase range2492728 1 1399 2646 BBa_K1891005_sequence 1 atgcgtgaatgcattagcattcacgtgggccaggccggtgtgcaaatcggtaatgcctgctgggagctgtattgtctggagcatggcatccagccggatggccagatgcctagcgacaaaaccattggcggcggcgatgatagtttcaataccttttttagcgagacaggtgccggcaagcatgtgccgcgtgccgtttttgtggatctggaaccgaccgtgatcgatgaagttcgcacaggtacctaccgtcagctgttccatccggagcagctgattaccggcaaagaggacgccgccaataactatgcacgcggccactacaccattggcaaagaaattattgatttagtgttagatcgtattcgcaagctggccgatcagtgtaccggcttacaaggctttctggtgtttcatagctttggcggcggtacaggtagcggctttaccagcctgttaatggaacgcctgagcgtggactacggcaagaaaagcaaactggaatttagtatttatccggccccgcaggtgagcaccgcagtggtggaaccttataacagcatcctgaccacccataccaccctggagcatagcgattgcgcatttatggtggacaacgaggcaatctatgacatctgccgccgcaatctggatattgagcgcccgacctacaccaacctgaaccgcctgattggccagatcgtgagcagcatcaccgccagtctgcgcttcgacggtgccctgaatgtggacctgaccgagtttcagaccaacctggttccgtatccgcgcattcatttccctctggccacctacgccccggtgattagtgccgaaaaagcctaccacgagcagctgagcgtggcagaaattaccaacgcctgcttcgagcctgcaaatcagatggtgaagtgcgatccgcgtcacggcaaatatatggcatgctgtctgctgtatcgcggcgatgttgtgccgaaagatgtgaatgccgccattgccaccatcaagaccaagcgtaccatccagtttgtggattggtgcccgaccggcttcaaagtgggcattaattaccagccgccgaccgttgttccgggcggtgatctggcaaaagttcagcgtgccgtgtgcatgctgagcaatacaaccgcaatcgccgaagcctgggcacgcctggaccacaagttcgacctgatgtatgccaagcgtgccttcgtgcattggtacgttggcgaaggtatggaggaaggcgagtttagcgaagcccgcgaagatatggccgcactggaaaaggactacgaggaagtgggcgtggatagcgttgaaggtgaaggcgaggaagaaggcgaagagtattaagctaccaccaccgctaccaccaccaccgctaccaccaccaccgaagacgccaaaaacataaagaaaggcccggcgccattctatccgctggaagatggaaccgctggagagcaactgcataaggctatgaagagatacgccctggttcctggaacaattgcttttacagatgcacatatcgaggtggacatcacttacgctgagtacttcgaaatgtccgttcggttggcagaagctatgaaacgatatgggctgaatacaaatcacagaatcgtcgtatgcagtgaaaactctcttcaattctttatgccggtgttgggcgcgttatttatcggagttgcagttgcgcccgcgaacgacatttataatgaacgtgaattgctcaacagtatgggcatttcgcagcctaccgtggtgttcgtttccaaaaaggggttgcaaaaaattttgaacgtgcaaaaaaagctcccaatcatccaaaaaattattatcatggattctaaaacggattaccagggatttcagtcgatgtacacgttcgtcacatctcatctacctcccggttttaatgaatacgattttgtgccagagtccttcgatagggacaagacaattgcactgatcatgaactcctctggatctactggtctgcctaaaggtgtcgctctgcctcatagaactgcctgcgtgagattctcgcatgccagagatcctatttttggcaatcaaatcattccggatactgcgattttaagtgttgttccattccatcacggttttggaatgtttactacactcggatatttgatatgtggatttcgagtcgtcttaatgtatagatttgaagaagagctgtttctgaggagccttcaggattacaagattcaaagtgcgctgctggtgccaaccctattctccttcttcgccaaaagcactctgattgacaaatacgatttatctaatttacacgaaattgcttctggtggcgctcccctctctaaggaagtcggggaagcggttgccaagaggttccatctgccaggtatcaggcaaggatatgggctcactgagactacatcagctattctgattacacccgagggggatgataaaccgggcgcggtcggtaaagttgttccattttttgaagcgaaggttgtggatctggataccgggaaaacgctgggcgttaatcaaagaggcgaactgtgtgtgagaggtcctatgattatgtccggttatgtaaacaatccggaagcgaccaacgccttgattgacaaggatggatga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z