LOCUS       BBa_K1893001            1932 bp    DNA     linear       18-May-2024
DEFINITION  Las receiver with GFP reporter (LasR+pLas+GFP)
ACCESSION   BBa_K1893001
VERSION     BBa_K1893001.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..784
                     /label=BBa_C0179
     stem_loop       804..847
                     /label=stem_loop
     misc_feature    793..872
                     /label=BBa_B0010
     terminator      793..872
                     /label=BBa_B0010
     stem_loop       888..907
                     /label=T7 TE
     misc_feature    914..914
                     /label=stop
     polyA_site      908..921
                     /label=polya
     misc_feature    881..921
                     /label=BBa_B0012
     terminator      881..921
                     /label=BBa_B0012
     terminator      793..921
                     /label=BBa_B0015
     promoter        930..1049
                     /label=pLas
     promoter        930..1049
                     /label=BBa_K1893030
     misc_feature    1..1049
                     /label=BBa_K1893000
     misc_feature    1062..1065
                     /label=conserved
     RBS             1058..1069
                     /label=BBa_B0034
     CDS             1076..1795
                     /label=GFP protein
     CDS             1076..1795
                     /label=BBa_E0040
     misc_feature    1058..1795
                     /label=BBa_I13500
     stem_loop       1815..1858
                     /label=stem_loop
     misc_feature    1804..1883
                     /label=BBa_B0010
     terminator      1804..1883
                     /label=BBa_B0010
     stem_loop       1899..1918
                     /label=T7 TE
     misc_feature    1925..1925
                     /label=stop
     polyA_site      1919..1932
                     /label=polya
     misc_feature    1892..1932
                     /label=BBa_B0012
     terminator      1892..1932
                     /label=BBa_B0012
     terminator      1804..1932
                     /label=BBa_B0015
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagaaagagg agaaatacta
       61 gatggccttg gttgacggtt ttcttgagct ggaacgctca agtggaaaat tggagtggag
      121 cgccatcctc cagaagatgg cgagcgacct tggattctcg aagatcctgt tcggcctgtt
      181 gcctaaggac agccaggact acgagaacgc cttcatcgtc ggcaactacc cggccgcctg
      241 gcgcgagcat tacgaccggg ctggctacgc gcgggtcgac ccgacggtca gtcactgtac
      301 ccagagcgta ctgccgattt tctgggaacc gtccatctac cagacgcgaa agcagcacga
      361 gttcttcgag gaagcctcgg ccgccggcct ggtgtatggg ctgaccatgc cgctgcatgg
      421 tgctcgcggc gaactcggcg cgctgagcct cagcgtggaa gcggaaaacc gggccgaggc
      481 caaccgtttc atagagtcgg tcctgccgac cctgtggatg ctcaaggact acgcactgca
      541 aagcggtgcc ggactggcct tcgaacatcc ggtcagcaaa ccggtggttc tgaccagccg
      601 ggagaaggaa gtgttgcagt ggtgcgccat cggcaagacc agttgggaga tatcggttat
      661 ctgcaactgc tcggaagcca atgtgaactt ccatatggga aatattcggc ggaagttcgg
      721 tgtgacctcc cgccgcgtag cggccattat ggccgttaat ttgggtctta ttactctcta
      781 ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
      841 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
      901 gtgggccttt ctgcgtttat atactagagc ctttccgaaa cgaaacaagt tggattttgc
      961 acctaccaga actggtagtt ctgacctgtg gctatcttcg aaggcatcga tattatgcac
     1021 attggaactc ttcatgacat aacgccgagt actagagaaa gaggagaaat actagatgcg
     1081 taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt
     1141 taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact
     1201 tacccttaaa tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac
     1261 tactttcggt tatggtgttc aatgctttgc gagataccca gatcatatga aacagcatga
     1321 ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga
     1381 tgacgggaac tacaagacac gtgctgaagt caagtttgaa ggtgataccc ttgttaatag
     1441 aatcgagtta aaaggtattg attttaaaga agatggaaac attcttggac acaaattgga
     1501 atacaactat aactcacaca atgtatacat catggcagac aaacaaaaga atggaatcaa
     1561 agttaacttc aaaattagac acaacattga agatggaagc gttcaactag cagaccatta
     1621 tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc
     1681 cacacaatct gccctttcga aagatcccaa cgaaaagaga gaccacatgg tccttcttga
     1741 gtttgtaaca gctgctggga ttacacatgg catggatgaa ctatacaaat aataatacta
     1801 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     1861 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
     1921 tctgcgttta ta
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