LOCUS BBa_K1895995 4232 bp DNA linear 12-May-2024 DEFINITION Time delay device ACCESSION BBa_K1895995 VERSION BBa_K1895995.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 CDS 227..955 /label=BBa_C0171 misc_feature 968..971 /label=conserved RBS 964..975 /label=BBa_B0034 CDS 982..1650 /label=cI 434 misc_feature 1612..1650 /label=LVA misc_feature 982..1650 /label=BBa_C0052 misc_feature 1651..1675 /label=Help:Barcodes CDS 982..1650 /label=BBa_C0052 misc_feature 1688..1691 /label=conserved RBS 1684..1695 /label=BBa_B0034 CDS 1702..2370 /label=cI 434 misc_feature 2332..2370 /label=LVA misc_feature 1702..2370 /label=BBa_C0052 misc_feature 2371..2395 /label=Help:Barcodes CDS 1702..2370 /label=BBa_C0052 misc_feature 2404..2442 /label=B1006 stem_loop 2413..2434 /label=modified thr terminator polyA_site 2435..2442 /label=PolyA polyA_site 2404..2412 /label=PolyA terminator 2404..2442 /label=BBa_B1006 misc_feature 2451..2470 /label=RhlR misc_feature 2451..2501 /label=modified P(Rhl) promoter 2471..2500 /label=-35 promoter 2451..2501 /label=BBa_I14017 misc_feature 2514..2517 /label=conserved RBS 2510..2521 /label=BBa_B0034 CDS 2531..3238 /label=cI lambda misc_feature 3239..3271 /label=LVA misc_feature 3278..3302 /label=Help:Barcodes CDS 2528..3302 /label=BBa_C0051 misc_feature 3311..3349 /label=B1006 stem_loop 3320..3341 /label=modified thr terminator polyA_site 3342..3349 /label=PolyA polyA_site 3311..3319 /label=PolyA terminator 3311..3349 /label=BBa_B1006 misc_feature 3413..3426 /label=OR1 434 misc_feature 3366..3382 /label=OR1 lambda misc_feature 3390..3406 /label=OR2 lambda promoter 3428..3433 /label=-10 promoter 3405..3410 /label=-35 promoter 3358..3439 /label=BBa_I12006 misc_feature 3452..3455 /label=conserved RBS 3448..3459 /label=BBa_B0034 misc_feature 3466..3468 /label=start CDS 3466..4185 /label=superfolder GFP coding region misc_feature 4180..4185 /label=stop CDS 3466..4185 /label=BBa_I746916 misc_feature 4194..4232 /label=B1006 stem_loop 4203..4224 /label=modified thr terminator polyA_site 4225..4232 /label=PolyA polyA_site 4194..4202 /label=PolyA terminator 4194..4232 /label=BBa_B1006 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga ggaatgacgg 241 aggctttttg ctgtggtggg acggtttgcg tagcgagatg cagccgatcc acgacagcca 301 gggcgtgttc gccgtcctgg aaaaggaagt gcggcgcctg ggcttcgatt actacgccta 361 tggcgtgcgc cacacgattc ccttcacccg gccgaagacc gaggtccatg gcacctatcc 421 caaggcctgg ctggagcgat accagatgca gaactacggg gccgtggatc cggcgatcct 481 caacggcctg cgctcctcgg aaatggtggt ctggagcgac agcctgttcg accagagccg 541 gatgctctgg aacgaggctc gcgattgggg cctctgtgtc ggcgcgacct tgccgatccg 601 cgcgccgaac aatttgctca gcgtgctttc cgtggcgcgc gaccagcaga acatctccag 661 cttcgagcgc gaggaaatcc gcctgcggct gcgttgcatg atcgagttgc tgacccagaa 721 gctgaccgac ctggagcatc cgatgctgat gtccaacccg gtctgcctga gccatcgcga 781 acgcgagatc ctgcaatgga ccgccgacgg caagagttcc ggggaaatcg ccatcatcct 841 gagcatctcc gagagcacgg tgaacttcca ccacaagaac atccagaaga agttcgacgc 901 gccgaacaag acgctggctg ccgcctacgc cgcggcgctg ggtctcatct aataatacta 961 gagaaagagg agaaatacta gatgagtatt tcttccaggg taaaaagcaa aagaatccag 1021 cttggactta accaggctga acttgctcaa aaggtgggga ctacccagca gtctatagag 1081 cagctcgaaa acggtaaaac taagcgacca cgctttttac cagaacttgc gtcagctctt 1141 ggcgtaagtg ttgactggct gctcaatggc acctctgatt cgaatgttag atttgttggg 1201 cacgttgagc ccaaagggaa atatccattg attagcatgg ttagagctgg ttcgtggtgt 1261 gaagcttgtg aaccctacga tatcaaggac attgatgaat ggtatgacag tgacgttaac 1321 ttattaggca atggattctg gctgaaggtt gaaggtgatt ccatgacctc acctgtaggt 1381 caaagcatcc ctgaaggtca tatggtgtta gtagatactg gacgggagcc agtgaatgga 1441 agccttgttg tagccaaact gactgacgcg aacgaagcaa cattcaagaa actggtcata 1501 gatggcggtc agaagtacct gaaaggcctg aatccttcat ggcctatgac tcctatcaac 1561 ggaaactgca agattatcgg tgttgtcgtg gaagcgaggg taaaattcgt agctgcaaac 1621 gacgaaaact acgctttagt agcttaataa ctctgatagt gctagtgtag atctctacta 1681 gagaaagagg agaaatacta gatgagtatt tcttccaggg taaaaagcaa aagaatccag 1741 cttggactta accaggctga acttgctcaa aaggtgggga ctacccagca gtctatagag 1801 cagctcgaaa acggtaaaac taagcgacca cgctttttac cagaacttgc gtcagctctt 1861 ggcgtaagtg ttgactggct gctcaatggc acctctgatt cgaatgttag atttgttggg 1921 cacgttgagc ccaaagggaa atatccattg attagcatgg ttagagctgg ttcgtggtgt 1981 gaagcttgtg aaccctacga tatcaaggac attgatgaat ggtatgacag tgacgttaac 2041 ttattaggca atggattctg gctgaaggtt gaaggtgatt ccatgacctc acctgtaggt 2101 caaagcatcc ctgaaggtca tatggtgtta gtagatactg gacgggagcc agtgaatgga 2161 agccttgttg tagccaaact gactgacgcg aacgaagcaa cattcaagaa actggtcata 2221 gatggcggtc agaagtacct gaaaggcctg aatccttcat ggcctatgac tcctatcaac 2281 ggaaactgca agattatcgg tgttgtcgtg gaagcgaggg taaaattcgt agctgcaaac 2341 gacgaaaact acgctttagt agcttaataa ctctgatagt gctagtgtag atctctacta 2401 gagaaaaaaa aaccccgccc ctgacagggc ggggtttttt tttactagag tcctgcatga 2461 attggtaggc ttacgcaaga aaatggtttg ttatagtcga atactagaga aagaggagaa 2521 atactagatg agcacaaaaa agaaaccatt aacacaagag cagcttgagg acgcacgtcg 2581 ccttaaagca atttatgaaa aaaagaaaaa tgaacttggc ttatcccagg aatctgtcgc 2641 agacaagatg gggatggggc agtcaggcgt tggtgcttta tttaatggca tcaatgcatt 2701 aaatgcttat aacgccgcat tgcttgcaaa aattctcaaa gttagcgttg aagaatttag 2761 cccttcaatc gccagagaaa tctacgagat gtatgaagcg gttagtatgc agccgtcact 2821 tagaagtgag tatgagtacc ctgttttttc tcatgttcag gcagggatgt tctcacctga 2881 gcttagaacc tttaccaaag gtgatgcgga gagatgggta agcacaacca aaaaagccag 2941 tgattctgca ttctggcttg aggttgaagg taattccatg accgcaccaa caggctccaa 3001 gccaagcttt cctgacggaa tgttaattct cgttgaccct gagcaggctg ttgagccagg 3061 tgatttctgc atagccagac ttgggggtga tgagtttacc ttcaagaaac tgatcaggga 3121 tagcggtcag gtgtttttac aaccactaaa cccacagtac ccaatgatcc catgcaatga 3181 gagttgttcc gttgtgggga aagttatcgc tagtcagtgg cctgaagaga cgtttggcgc 3241 tgcaaacgac gaaaactacg ctttagtagc ttaataacgc tgatagtgct agtgtagatc 3301 gctactagag aaaaaaaaac cccgcccctg acagggcggg gttttttttt actagaggca 3361 accattatca ccgccagagg taaaatagtc aacacgcacg gtgttagata ttacaaactt 3421 tcttgtatag atttaacgtt actagagaaa gaggagaaat actagatgcg taaaggcgaa 3481 gagctgttca ctggtgtcgt ccctattctg gtggaactgg atggtgatgt caacggtcat 3541 aagttttccg tgcgtggcga gggtgaaggt gacgcaacta atggtaaact gacgctgaag 3601 ttcatctgta ctactggtaa actgccggta ccttggccga ctctggtaac gacgctgact 3661 tatggtgttc agtgctttgc tcgttatccg gaccatatga agcagcatga cttcttcaag 3721 tccgccatgc cggaaggcta tgtgcaggaa cgcacgattt cctttaagga tgacggcacg 3781 tacaaaacgc gtgcggaagt gaaatttgaa ggcgataccc tggtaaaccg cattgagctg 3841 aaaggcattg actttaaaga agacggcaat atcctgggcc ataagctgga atacaatttt 3901 aacagccaca atgtttacat caccgccgat aaacaaaaaa atggcattaa agcgaatttt 3961 aaaattcgcc acaacgtgga ggatggcagc gtgcagctgg ctgatcacta ccagcaaaac 4021 actccaatcg gtgatggtcc tgttctgctg ccagacaatc actatctgag cacgcaaagc 4081 gttctgtcta aagatccgaa cgagaaacgc gatcatatgg ttctgctgga gttcgtaacc 4141 gcagcgggca tcacgcatgg tatggatgaa ctgtacaaat gatgatacta gagaaaaaaa 4201 aaccccgccc ctgacagggc ggggtttttt tt //