LOCUS       BBa_K1899005            1752 bp    DNA     linear       17-May-2024
DEFINITION  J23101-B0032-TetR-B1006- <i>phlFp</i> -GFP
ACCESSION   BBa_K1899005
VERSION     BBa_K1899005.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     RBS             44..56
                     /label=RBS-3\Weak
     RBS             50..53
                     /label=RBS
     misc_feature    44..56
                     /label=BBa_B0032
     RBS             44..56
                     /label=BBa_B0032
     CDS             66..682
                     /label=tetR
     misc_feature    683..716
                     /label=SsrA
     misc_feature    723..747
                     /label=Help:Barcodes
     CDS             63..747
                     /label=BBa_C0040
     misc_feature    756..794
                     /label=B1006
     stem_loop       765..786
                     /label=modified thr terminator
     polyA_site      787..794
                     /label=PolyA
     polyA_site      756..764
                     /label=PolyA
     terminator      756..794
                     /label=BBa_B1006
     promoter        803..868
                     /label=BBa_K1899004
     RBS             877..889
                     /label=RBS-3\Weak
     RBS             883..886
                     /label=RBS
     misc_feature    877..889
                     /label=BBa_B0032
     RBS             877..889
                     /label=BBa_B0032
     CDS             896..1615
                     /label=GFP protein
     CDS             896..1615
                     /label=BBa_E0040
     stem_loop       1635..1678
                     /label=stem_loop
     misc_feature    1624..1703
                     /label=BBa_B0010
     terminator      1624..1703
                     /label=BBa_B0010
     stem_loop       1719..1738
                     /label=T7 TE
     misc_feature    1745..1745
                     /label=stop
     polyA_site      1739..1752
                     /label=polya
     misc_feature    1712..1752
                     /label=BBa_B0012
     terminator      1712..1752
                     /label=BBa_B0012
     misc_feature    877..1752
                     /label=BBa_E0240
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagtcacaca ggaaagtact
       61 agatgtccag attagataaa agtaaagtga ttaacagcgc attagagctg cttaatgagg
      121 tcggaatcga aggtttaaca acccgtaaac tcgcccagaa gctaggtgta gagcagccta
      181 cattgtattg gcatgtaaaa aataagcggg ctttgctcga cgccttagcc attgagatgt
      241 tagataggca ccatactcac ttttgccctt tagaagggga aagctggcaa gattttttac
      301 gtaataacgc taaaagtttt agatgtgctt tactaagtca tcgcgatgga gcaaaagtac
      361 atttaggtac acggcctaca gaaaaacagt atgaaactct cgaaaatcaa ttagcctttt
      421 tatgccaaca aggtttttca ctagagaatg cattatatgc actcagcgct gtggggcatt
      481 ttactttagg ttgcgtattg gaagatcaag agcatcaagt cgctaaagaa gaaagggaaa
      541 cacctactac tgatagtatg ccgccattat tacgacaagc tatcgaatta tttgatcacc
      601 aaggtgcaga gccagccttc ttattcggcc ttgaattgat catatgcgga ttagaaaaac
      661 aacttaaatg tgaaagtggg tccgctgcaa acgacgaaaa ctacgcttta gtagcttaat
      721 aacactgata gtgctagtgt agatcactac tagagaaaaa aaaaccccgc ccctgacagg
      781 gcggggtttt tttttactag agcgacgtac ggtggaatct gattcgttac caattgacat
      841 gatacgaaac gtaccgtatc gttaaggtta ctagagtcac acaggaaagt actagatgcg
      901 taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt
      961 taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact
     1021 tacccttaaa tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac
     1081 tactttcggt tatggtgttc aatgctttgc gagataccca gatcatatga aacagcatga
     1141 ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga
     1201 tgacgggaac tacaagacac gtgctgaagt caagtttgaa ggtgataccc ttgttaatag
     1261 aatcgagtta aaaggtattg attttaaaga agatggaaac attcttggac acaaattgga
     1321 atacaactat aactcacaca atgtatacat catggcagac aaacaaaaga atggaatcaa
     1381 agttaacttc aaaattagac acaacattga agatggaagc gttcaactag cagaccatta
     1441 tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc
     1501 cacacaatct gccctttcga aagatcccaa cgaaaagaga gaccacatgg tccttcttga
     1561 gtttgtaaca gctgctggga ttacacatgg catggatgaa ctatacaaat aataatacta
     1621 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     1681 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
     1741 tctgcgttta ta
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