LOCUS       BBa_K1899006            2220 bp    DNA     linear       17-May-2024
DEFINITION  J23101-B0032-lacl-B1006- <i>phlFp</i>-GFP
ACCESSION   BBa_K1899006
VERSION     BBa_K1899006.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     RBS             44..56
                     /label=RBS-3\Weak
     RBS             50..53
                     /label=RBS
     misc_feature    44..56
                     /label=BBa_B0032
     RBS             44..56
                     /label=BBa_B0032
     misc_feature    1152..1190
                     /label=LVA
     CDS             63..1190
                     /label=lacI-LVA
     misc_feature    63..1190
                     /label=BBa_C0012
     misc_feature    1191..1215
                     /label=Help:Barcodes
     CDS             63..1190
                     /label=BBa_C0012
     misc_feature    1224..1262
                     /label=B1006
     stem_loop       1233..1254
                     /label=modified thr terminator
     polyA_site      1255..1262
                     /label=PolyA
     polyA_site      1224..1232
                     /label=PolyA
     terminator      1224..1262
                     /label=BBa_B1006
     promoter        1271..1336
                     /label=BBa_K1899004
     RBS             1345..1357
                     /label=RBS-3\Weak
     RBS             1351..1354
                     /label=RBS
     misc_feature    1345..1357
                     /label=BBa_B0032
     RBS             1345..1357
                     /label=BBa_B0032
     CDS             1364..2083
                     /label=GFP protein
     CDS             1364..2083
                     /label=BBa_E0040
     stem_loop       2103..2146
                     /label=stem_loop
     misc_feature    2092..2171
                     /label=BBa_B0010
     terminator      2092..2171
                     /label=BBa_B0010
     stem_loop       2187..2206
                     /label=T7 TE
     misc_feature    2213..2213
                     /label=stop
     polyA_site      2207..2220
                     /label=polya
     misc_feature    2180..2220
                     /label=BBa_B0012
     terminator      2180..2220
                     /label=BBa_B0012
     misc_feature    1345..2220
                     /label=BBa_E0240
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagtcacaca ggaaagtact
       61 agatggtgaa tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt
      121 atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa
      181 aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg
      241 cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt
      301 cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt
      361 cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc
      421 aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg
      481 aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca
      541 acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat ctggtcgcat
      601 tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc
      661 gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg
      721 aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca
      781 tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca
      841 ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac gacgataccg
      901 aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg
      961 ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc
     1021 agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg
     1081 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     1141 aaagcgggca ggctgcaaac gacgaaaact acgctttagt agcttaataa ctctgatagt
     1201 gctagtgtag atctctacta gagaaaaaaa aaccccgccc ctgacagggc ggggtttttt
     1261 tttactagag cgacgtacgg tggaatctga ttcgttacca attgacatga tacgaaacgt
     1321 accgtatcgt taaggttact agagtcacac aggaaagtac tagatgcgta aaggagaaga
     1381 acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa
     1441 attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt
     1501 tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta ctttcggtta
     1561 tggtgttcaa tgctttgcga gatacccaga tcatatgaaa cagcatgact ttttcaagag
     1621 tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg acgggaacta
     1681 caagacacgt gctgaagtca agtttgaagg tgataccctt gttaatagaa tcgagttaaa
     1741 aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat acaactataa
     1801 ctcacacaat gtatacatca tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa
     1861 aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc aacaaaatac
     1921 tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca cacaatctgc
     1981 cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt ttgtaacagc
     2041 tgctgggatt acacatggca tggatgaact atacaaataa taatactaga gccaggcatc
     2101 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     2161 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//