LOCUS       BBa_K1903013            1700 bp    DNA     linear       01-Jun-2024
DEFINITION  Plac-RBS-Split TEV-IntN DnaE + PA1-RBS + Split TEV-IntC DnaE
ACCESSION   BBa_K1903013
VERSION     BBa_K1903013.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             229..582
                     /label=N-terminal half of TEV Protease
     CDS             229..582
                     /label=BBa_K1159101
     misc_feature    591..892
                     /label=BBa_K1362400
     stem_loop       912..955
                     /label=stem_loop
     misc_feature    901..980
                     /label=BBa_B0010
     terminator      901..980
                     /label=BBa_B0010
     stem_loop       996..1015
                     /label=T7 TE
     misc_feature    1022..1022
                     /label=stop
     polyA_site      1016..1029
                     /label=polya
     misc_feature    989..1029
                     /label=BBa_B0012
     terminator      989..1029
                     /label=BBa_B0012
     terminator      901..1029
                     /label=BBa_B0015
     CDS             1..1029
                     /label=BBa_K1903006
     promoter        1038..1072
                     /label=BBa_J23101
     misc_feature    1085..1088
                     /label=conserved
     RBS             1081..1092
                     /label=BBa_B0034
     misc_feature    1101..1201
                     /label=BBa_K1362401
     CDS             1210..1563
                     /label=C-terminal half of TEV Protease
     misc_feature    1510..1512
                     /label=Ser --> Val for autolysis resistance
     CDS             1210..1563
                     /label=BBa_K1159102
     stem_loop       1583..1626
                     /label=stem_loop
     misc_feature    1572..1651
                     /label=BBa_B0010
     terminator      1572..1651
                     /label=BBa_B0010
     stem_loop       1667..1686
                     /label=T7 TE
     misc_feature    1693..1693
                     /label=stop
     polyA_site      1687..1700
                     /label=polya
     misc_feature    1660..1700
                     /label=BBa_B0012
     terminator      1660..1700
                     /label=BBa_B0012
     terminator      1572..1700
                     /label=BBa_B0015
     CDS             1038..1700
                     /label=BBa_K1903008
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagaggg agaaagcttg
      241 tttaaggggc cgcgtgatta caacccgata tcgagcacca tttgtcattt gacgaatgaa
      301 tctgatgggc acacaacatc gttgtatggt attggatttg gtcccttcat cattacaaac
      361 aagcacttgt ttagaagaaa taatggaaca ctgttggtcc aatcactaca tggtgtattc
      421 aaggtcaaga acaccacgac tttgcaacaa cacctcattg atgggaggga catgataatt
      481 attcgcatgc ctaaggattt cccaccattt cctcaaaagc tgaaatttag agagccacaa
      541 agggaagagc gcatatgtct tgtgacaacc aacttccaaa cttactagag taagctatga
      601 aacggaaata ttgacagtag aatatggatt attaccgatt ggtaaaattg tagaaaagcg
      661 catcgaatgt actgtttata gcgttgataa taatggaaat atttatacac aacctgtagc
      721 acaatggcac gatcgcggag aacaagaggt gtttgagtat tgtttggaag atggttcatt
      781 gattcgggca acaaaagacc ataagtttat gactgttgat ggtcaaatgt tgccaattga
      841 tgaaatattt gaacgtgaat tggatttgat gcgggttgat aatttgccga attactagag
      901 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
      961 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
     1021 gcgtttatat actagagttt acagctagct cagtcctagg tattatgcta gctactagag
     1081 aaagaggaga aatactagag atcaaaatag ccacacgtaa atatttaggc aaacaaaatg
     1141 tctatgacat tggagttgag cgcgaccata attttgcact caaaaatggc ttcatagctt
     1201 ctactagaga agagcatgtc tagcatggtg tcagacacta gctgcacatt cccttcatct
     1261 gatggcatat tctggaagca ttggattcaa accaaggatg ggcagtgtgg cagtccatta
     1321 gtatcaacta gagatgggtt cattgttggt atacactcag catcgaattt caccaacaca
     1381 aacaattatt tcacaagcgt gccgaaaaac ttcatggaat tgttgacaaa tcaggaggcg
     1441 cagcagtggg ttagtggttg gcgattaaat gctgactcag tattgtgggg gggccataaa
     1501 gttttcatgg tgaaacctga agagcctttt cagccagtta aggaagcgac tcaactcatg
     1561 aattactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     1621 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
     1681 tgggcctttc tgcgtttata
//