LOCUS BBa_K1911004 4960 bp DNA linear 13-May-2024 DEFINITION pLamdaR-LacI-GFP-LAA-pLac-ClpXP-CI ACCESSION BBa_K1911004 VERSION BBa_K1911004.1 FEATURES Location/Qualifiers promoter 38..43 /label=-10 promoter 15..20 /label=-35 misc_feature 25..41 /label=OR1 misc_feature 1..17 /label=OR2 misc_feature 1..49 /label=BBa_R0051 promoter 1..49 /label=BBa_R0051 RBS 58..68 /label=RBS-4\Weaker RBS 64..67 /label=RBS misc_feature 58..68 /label=BBa_B0033 RBS 58..68 /label=BBa_B0033 misc_feature 1164..1202 /label=LVA CDS 75..1202 /label=lacI-LVA misc_feature 75..1202 /label=BBa_C0012 misc_feature 1203..1227 /label=Help:Barcodes CDS 75..1202 /label=BBa_C0012 misc_feature 1240..1243 /label=conserved RBS 1236..1247 /label=BBa_B0034 CDS 1254..1970 /label=BBa_K1911005 misc_feature 1979..2011 /label=WT SsrA tag AANDENYALAA misc_feature 1979..2011 /label=BBa_M0050 stem_loop 2031..2074 /label=stem_loop misc_feature 2020..2099 /label=BBa_B0010 terminator 2020..2099 /label=BBa_B0010 stem_loop 2115..2134 /label=T7 TE misc_feature 2141..2141 /label=stop polyA_site 2135..2148 /label=polya misc_feature 2108..2148 /label=BBa_B0012 terminator 2108..2148 /label=BBa_B0012 terminator 2020..2148 /label=BBa_B0015 misc_feature 2159..2175 /label=lac O1 promoter 2176..2181 /label=-35 misc_feature 2182..2198 /label=lac O1 promoter 2199..2204 /label=-10 misc_feature 2157..2210 /label=BBa_R0011 promoter 2157..2210 /label=BBa_R0011 RBS 2220..2230 /label=RBS-4\Weaker RBS 2226..2229 /label=RBS misc_feature 2220..2230 /label=BBa_B0033 RBS 2220..2230 /label=BBa_B0033 CDS 2237..3511 /label=BBa_K1911003 RBS 3520..3530 /label=RBS-4\Weaker RBS 3526..3529 /label=RBS misc_feature 3520..3530 /label=BBa_B0033 RBS 3520..3530 /label=BBa_B0033 CDS 3537..4160 /label=BBa_K1911002 RBS 4169..4179 /label=RBS-4\Weaker RBS 4175..4178 /label=RBS misc_feature 4169..4179 /label=BBa_B0033 RBS 4169..4179 /label=BBa_B0033 CDS 4189..4896 /label=cI lambda misc_feature 4897..4929 /label=LVA misc_feature 4936..4960 /label=Help:Barcodes CDS 4186..4960 /label=BBa_C0051 ORIGIN 1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagtca 61 cacaggacta ctagatggtg aatgtgaaac cagtaacgtt atacgatgtc gcagagtatg 121 ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca ggccagccac gtttctgcga 181 aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa ttacattccc aaccgcgtgg 241 cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc agtctggccc 301 tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa ctgggtgcca 361 gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg gcggtgcaca 421 atcttctcgc gcaacgcgtc agtgggctga tcattaacta tccgctggat gaccaggatg 481 ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt atttcttgat gtctctgacc 541 agacacccat caacagtatt attttctccc atgaagacgg tacgcgactg ggcgtggagc 601 atctggtcgc attgggtcac cagcaaatcg cgctgttagc gggcccatta agttctgtct 661 cggcgcgtct gcgtctggct ggctggcata aatatctcac tcgcaatcaa attcagccga 721 tagcggaacg ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc atgcaaatgc 781 tgaatgaggg catcgttccc actgcgatgc tggttgccaa cgatcagatg gcgctgggcg 841 caatgcgcgc cattaccgag tccgggctgc gcgttggtgc ggatatctcg gtagtgggat 901 acgacgatac cgaagacagc tcatgttata tcccgccgtt aaccaccatc aaacaggatt 961 ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca actctctcag ggccaggcgg 1021 tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc ctggcgccca 1081 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg 1141 tttcccgact ggaaagcggg caggctgcaa acgacgaaaa ctacgcttta gtagcttaat 1201 aactctgata gtgctagtgt agatctctac tagagaaaga ggagaaatac tagatggcta 1261 gcaaaggaga agaactcttc actggagttg tcccaattct tgttgaatta gatggtgatg 1321 ttaacggcca caagttctct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac 1381 ttaccctgaa gttcatctgc actactggca aactgcctgt tccatggcca accctggtca 1441 ctactctgtg ctatggtgtt caatgctttt caagataccc ggatcatatg aaacggcatg 1501 actttttcaa gagtgccatg cccgaaggtt atgtacagga aaggaccatc ttcttcaaag 1561 atgacggcaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata 1621 gaatcgagtt aaaaggtatt gacttcaagg aagatggcaa cattctggga cacaaattgg 1681 aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca 1741 aagtgaactt caagacccgc cacaacattg aagatggaag cgttcaacta gcagaccatt 1801 atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt 1861 ccacacaatc tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg 1921 agtttgtaac agctgctggg attacacatg gcatggatga actgtacaat tactagaggc 1981 tgctaacgac gaaaactacg ctctggctgc ttactagagc caggcatcaa ataaaacgaa 2041 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 2101 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagaatt 2161 gtgagcggat aacaattgac attgtgagcg gataacaaga tactgagcac atactagagt 2221 cacacaggac tactagatga cagataaacg caaagatggc tcaggcaaat tgctgtattg 2281 ctctttttgc ggcaaaagcc agcatgaagt gcgcaagctg attgccggtc catccgtgta 2341 tatctgcgac gaatgtgttg atttatgtaa cgacatcatt cgcgaagaga ttaaagaagt 2401 tgcaccgcat cgtgaacgca gtgcgctacc gacgccgcat gaaattcgca accacctgga 2461 cgattacgtt atcggccagg aacaggcgaa aaaagtgctg gcggtcgcgg tatacaacca 2521 ttacaaacgt ctgcgcaacg gcgataccag caatggcgtc gagttgggca aaagtaacat 2581 tctgctgatc ggtccgaccg gttccggtaa aacgctgctg gctgaaacgc tggcgcgcct 2641 gctggatgtt ccgttcacca tggccgacgc gactacactg accgaagccg gttatgtggg 2701 tgaagacgtt gaaaacatca ttcagaagct gttgcagaaa tgcgactacg atgtccagaa 2761 agcacagcgt ggtattgtct acatcgatga aatcgacaag atttctcgta agtcagacaa 2821 cccgtccatt acccgagacg tttccggtga aggcgtacag caggcactgt tgaaactgat 2881 cgaaggtacg gtagctgctg ttccaccgca aggtgggcgt aaacatccgc agcaggagtt 2941 cttgcaggtt gatacctcta agatcctgtt tatttgtggc ggtgcgtttg ccggtctgga 3001 taaagtgatt tcccaccgtg tagaaaccgg ctccggcatt ggttttggcg cgacggtaaa 3061 agcgaagtcc gacaaagcaa gcgaaggcga gctgctggcg caggttgaac cggaagatct 3121 gatcaagttt ggtcttatcc ctgagtttat tggtcgtctg ccggttgtcg caacgttgaa 3181 tgaactgagc gaagaagctc tgattcagat cctcaaagag ccgaaaaacg ccctgaccaa 3241 gcagtatcag gcgctgttta atctggaagg cgtggatctg gagttccgtg acgaggcgct 3301 ggatgctatc gctaagaaag cgatggcgcg taaaaccggt gcccgtggcc tgcgttccat 3361 cgtagaagcc gcactgctcg ataccatgta cgatctgccg tccatggaag acgtcgaaaa 3421 agtggttatc gacgagtcgg taattgatgg tcaaagcaaa ccgttgctga tttatggcaa 3481 gccggaagcg caacaggcat ctggtgaata atactagagt cacacaggac tactagatgt 3541 catacagcgg cgaacgagat aactttgcac cccatatggc gctggtgccg atggtcattg 3601 aacagacctc acgcggtgag cgctcttttg atatctattc tcgtctactt aaggaacgcg 3661 tcatttttct gactggccag gttgaagacc acatggctaa cctgattgtg gcgcagatgc 3721 tgttcctgga agcggaaaac ccagaaaaag atatctatct gtacattaac tccccaggcg 3781 gggtgatcac tgccgggatg tctatctatg acaccatgca gtttatcaag cctgatgtca 3841 gcaccatctg tatgggccag gcggcctcga tgggcgcttt cttgctgacc gcaggggcaa 3901 aaggtaaacg tttttgcctg ccaaattcgc gcgtgatgat tcaccaaccg ttgggcggct 3961 accagggcca ggcgaccgat atcgaaattc atgcccgtga aattctgaaa gttaaagggc 4021 gcatgaatga acttatggcg cttcatacgg gtcaatcatt agaacagatt gaacgtgata 4081 ccgagcgcga tcgcttcctt tccgcccctg aagcggtgga atacggtctg gtcgattcga 4141 ttctgaccca tcgtaattga tactagagtc acacaggact actagatgag cacaaaaaag 4201 aaaccattaa cacaagagca gcttgaggac gcacgtcgcc ttaaagcaat ttatgaaaaa 4261 aagaaaaatg aacttggctt atcccaggaa tctgtcgcag acaagatggg gatggggcag 4321 tcaggcgttg gtgctttatt taatggcatc aatgcattaa atgcttataa cgccgcattg 4381 cttgcaaaaa ttctcaaagt tagcgttgaa gaatttagcc cttcaatcgc cagagaaatc 4441 tacgagatgt atgaagcggt tagtatgcag ccgtcactta gaagtgagta tgagtaccct 4501 gttttttctc atgttcaggc agggatgttc tcacctgagc ttagaacctt taccaaaggt 4561 gatgcggaga gatgggtaag cacaaccaaa aaagccagtg attctgcatt ctggcttgag 4621 gttgaaggta attccatgac cgcaccaaca ggctccaagc caagctttcc tgacggaatg 4681 ttaattctcg ttgaccctga gcaggctgtt gagccaggtg atttctgcat agccagactt 4741 gggggtgatg agtttacctt caagaaactg atcagggata gcggtcaggt gtttttacaa 4801 ccactaaacc cacagtaccc aatgatccca tgcaatgaga gttgttccgt tgtggggaaa 4861 gttatcgcta gtcagtggcc tgaagagacg tttggcgctg caaacgacga aaactacgct 4921 ttagtagctt aataacgctg atagtgctag tgtagatcgc //