Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Xinyi Liu
Date created: 2016-10-08 11:00:00
Date modified: 2016-10-19 01:22:24
The SNAP UNIT: SNAP-tag flanked by loxP
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1926011_sequence (Version 1) |
Description
This part is the SNAP UNIT of cyclebow system including an sv40 nuclear location sequence (NLS), a covalent labeling of fusion proteins named SNAP-tag?? and an sv40 terminator flanked by homodromous recognition site of recombinase CRE named loxP. The whole part can be cut off by CRE.You may use this part to:
1) Localize proteins in vivo with BLOCK and dye from NEB company;
2) Dye after blocking, you are able to see whether an event have an influence on the localization and expression of the interested protein;
3) Fast cut off this part by transient transfecting recombinase gene into nucleus.