BBa_K1926011 1 BBa_K1926011 The SNAP UNIT: SNAP-tag flanked by loxP 2016-10-08T11:00:00Z 2016-10-19T01:22:24Z The SNAP-tag?? was brought from NEB company. NLS and sv40 terminator were got from commercial plasmid: pentry nls GFP/pcdna3.1. This part is the SNAP UNIT of cyclebow system including an sv40 nuclear location sequence (NLS), a covalent labeling of fusion proteins named SNAP-tag?? and an sv40 terminator flanked by homodromous recognition site of recombinase CRE named loxP. The whole part can be cut off by CRE. You may use this part to: 1) Localize proteins in vivo with BLOCK and dye from NEB company; 2) Dye after blocking, you are able to see whether an event have an influence on the localization and expression of the interested protein; 3) Fast cut off this part by transient transfecting recombinase gene into nucleus. false false _2393_ 28998 28998 9 false None false Xinyi Liu annotation2491776 1 loxP range2491776 1 755 788 annotation2491779 1 sv40 terminator range2491779 1 605 754 annotation2491738 1 SNAP-tag range2491738 1 59 604 annotation2491851 1 TAA range2491851 1 626 628 annotation2491774 1 loxP range2491774 1 1 34 annotation2491778 1 NLS range2491778 1 38 58 BBa_K1926021 1 BBa_K1926021 The SNAP-VIKA UNIT 2016-10-09T11:00:00Z 2016-10-11T06:21:46Z The SNAP-tag?? was brought from NEB company. NLS and sv40 terminator were got from commercial plasmid: pentry nls GFP/pcdna3.1. The recombinase gene VIKA and its RTS Vox were got through denovo synthesis. This part is the SNAP-VIKA UNIT of cyclebow including a SNAP UNIT and a VIKA UNIT. The SNAP UNIT, which can be cut off by CRE, includes an sv40 nuclear location sequence (NLS), a covalent labeling of fusion proteins named SNAP-tag?? and an sv40 terminator flanked by homodromous recognition site of recombinase CRE named loxP. The VIKA UNIT, which can be cut off by VIKA, includes an sv40 nuclear location sequence (NLS), a recombinase gene named VIKA and an sv40 terminator flanked by homodromous recognition site of recombinase VIKA named Vox. You may use this part to: 1) Test the cut-off efficiency of recombinase CRE and VIKA by qRT-PCR; 2) Estimate the time needed for genome repair because VIKA can only be expressed after the cut off of SNAP UNIT and genome repair; 3) Fast cut off this part by transient transfecting recombinase gene into nucleus; 4) When there is no CRE, you can use this part as the SNAP UNIT, which can help localize proteins in vivo with BLOCK and dye from NEB company. false false _2393_ 28998 28998 9 false None false Xinyi Liu component2491913 1 BBa_K1926011 component2491919 1 BBa_K1926012 annotation2491913 1 BBa_K1926011 range2491913 1 1 788 annotation2491919 1 BBa_K1926012 range2491919 1 789 2082 BBa_K1926012 1 BBa_K1926012 The VIKA UNIT: recombinase VIKA flanked by Vox 2016-10-08T11:00:00Z 2016-10-11T03:47:23Z The recombinase gene VIKA and its RTS Vox were got through denovo synthesis. NLS and sv40 terminator were got from commercial plasmid: pentry nls GFP/pcdn3.1. This part is the VIKA UNIT of cyclebow system including an sv40 nuclear location sequence (NLS), a recombinase gene named VIKA and an sv40 terminator flanked by homodromous recognition site of recombinase VIKA named Vox. The whole part can be cut off by VIKA. This part is the improvement of the BBa_K1641003，which is a plain VIKA. You may use this part to: 1) Test the cut-off efficiency of recombinase VIKA by qRT-PCR; 2) Fast cut off this part by transient transfecting recombinase gene into nucleus; 3) Do lineage tracking. false false _2393_ 28998 28998 9 false None false Xinyi Liu annotation2491854 1 Vox range2491854 1 1 34 annotation2491856 1 Vox range2491856 1 1261 1294 annotation2491858 1 TAA range2491858 1 1139 1141 annotation2491857 1 sv40 terminator range2491857 1 1118 1260 annotation2491855 1 VIKA range2491855 1 35 1117 BBa_K1926021_sequence 1 ataacttcgtatagcatacattatacgaagttatatgcctaagaagaagaggaaggtcatggacaaagactgcgaaatgaagcgcaccaccctggatagccctctgggcaagctggaactgtctgggtgcgaacagggcctgcaccgtatcatcttcctgggcaaaggaacatctgccgccgacgccgtggaagtgcctgccccagccgccgtgctgggcggaccagagccactgatgcaggccaccgcctggctcaacgcctactttcaccagcctgaggccatcgaggagttccctgtgccagccctgcaccacccagtgttccagcaggagagctttacccgccaggtgctgtggaaactgctgaaagtggtgaagttcggagaggtcatcagctacagccacctggccgccctggccggcaatcccgccgccaccgccgccgtgaaaaccgccctgagcggaaatcccgtgcccattctgatcccctgccaccgggtggtgcagggcgacctggacgtggggggctacgagggcgggctcgccgtgaaagagtggctgctggcccacgagggccacagactgggcaagcctgggctgggtcaacatgttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtataccgtcgaggtaataacttcgtatagcatacattatacgaagttataataggtccgagaatgacagttctcagaaattgaatgaccgatctgactccgtttccaccgttggaacatctggaacctgatgaatttgccgacttagtcagaaaagcgattaagcgcgatccacaagccggtgcgcaccctgcaattcaaagcgcgatcagtcattttcaggacgaatttgtacgtagacaaggcgaatggcaaccagctacattacagcgtttgagaaatgcatggaacgtttttgtgagatggtgtacgcaccaaggtattccggctctgcctgcacgtcatcaggatgttgaacgttatctgatcgaacgccgtaatgaattacaccgcaacaccctgaaagttcatttatgggccattggcaagactcacgtgatcagtggcctgcctaatccatgcgcgcatcgttacgtcaaagcccaaatggcgcagattactcatcaaaaagtacgcgaacgtgaaagaatcgaacaggctcctgcattccgcgaatccgatctggaccgtttaaccgaattgtggtccgctacaagatcggtcacgcaacagcgcgatttgatgattgtttctctggcttatgaaactctgttgcgtaagaacaacctggaacaaatgaaagtgggtgatattgaattttgtcaggacggcagcgccctgattacaatccctttctctaaaacgaaccactcaggtcgcgatgacgtccgttggattagtccacaagtcgctaatcaggtacatgcatacctgcaattacctaacatcgatgccgacccacaatgctttttgctgcaacgtgttaaacgttccggcaaggcgttgaatccagaatcgcataataccctgaacggccatcacccggttagcgaaaaattaattagtagagtgttcgaaagagcttggcgcgcattgaaccacgaaactggtccacgttatactggccattctgcaagagtgggtgctgcacaagatttattgcaggaaggctactcaacactgcaagttatgcaggctggtggctggtcttcagaaaaaatggtgttacgttatggcagacatttgcacgcacatacgtctgctatggctcaaaagagacgccagcgccaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtataccgtcgaaataggtccgagaatgacagttctcagaaattga BBa_K1926011_sequence 1 ataacttcgtatagcatacattatacgaagttatatgcctaagaagaagaggaaggtcatggacaaagactgcgaaatgaagcgcaccaccctggatagccctctgggcaagctggaactgtctgggtgcgaacagggcctgcaccgtatcatcttcctgggcaaaggaacatctgccgccgacgccgtggaagtgcctgccccagccgccgtgctgggcggaccagagccactgatgcaggccaccgcctggctcaacgcctactttcaccagcctgaggccatcgaggagttccctgtgccagccctgcaccacccagtgttccagcaggagagctttacccgccaggtgctgtggaaactgctgaaagtggtgaagttcggagaggtcatcagctacagccacctggccgccctggccggcaatcccgccgccaccgccgccgtgaaaaccgccctgagcggaaatcccgtgcccattctgatcccctgccaccgggtggtgcagggcgacctggacgtggggggctacgagggcgggctcgccgtgaaagagtggctgctggcccacgagggccacagactgggcaagcctgggctgggtcaacatgttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtataccgtcgaggtaataacttcgtatagcatacattatacgaagttat BBa_K1926012_sequence 1 aataggtccgagaatgacagttctcagaaattgaatgaccgatctgactccgtttccaccgttggaacatctggaacctgatgaatttgccgacttagtcagaaaagcgattaagcgcgatccacaagccggtgcgcaccctgcaattcaaagcgcgatcagtcattttcaggacgaatttgtacgtagacaaggcgaatggcaaccagctacattacagcgtttgagaaatgcatggaacgtttttgtgagatggtgtacgcaccaaggtattccggctctgcctgcacgtcatcaggatgttgaacgttatctgatcgaacgccgtaatgaattacaccgcaacaccctgaaagttcatttatgggccattggcaagactcacgtgatcagtggcctgcctaatccatgcgcgcatcgttacgtcaaagcccaaatggcgcagattactcatcaaaaagtacgcgaacgtgaaagaatcgaacaggctcctgcattccgcgaatccgatctggaccgtttaaccgaattgtggtccgctacaagatcggtcacgcaacagcgcgatttgatgattgtttctctggcttatgaaactctgttgcgtaagaacaacctggaacaaatgaaagtgggtgatattgaattttgtcaggacggcagcgccctgattacaatccctttctctaaaacgaaccactcaggtcgcgatgacgtccgttggattagtccacaagtcgctaatcaggtacatgcatacctgcaattacctaacatcgatgccgacccacaatgctttttgctgcaacgtgttaaacgttccggcaaggcgttgaatccagaatcgcataataccctgaacggccatcacccggttagcgaaaaattaattagtagagtgttcgaaagagcttggcgcgcattgaaccacgaaactggtccacgttatactggccattctgcaagagtgggtgctgcacaagatttattgcaggaaggctactcaacactgcaagttatgcaggctggtggctggtcttcagaaaaaatggtgttacgttatggcagacatttgcacgcacatacgtctgctatggctcaaaagagacgccagcgccaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtataccgtcgaaataggtccgagaatgacagttctcagaaattga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z